Differential expression of glycogenes in tonsillar B lymphocytes in association with proteinuria and renal dysfunction in IgA nephropathy

Tatsuyuki Inoue, Hitoshi Sugiyama, Yoshiyuki Hiki, Keiichi Takiue, Hiroshi Morinaga, Masashi Kitagawa, Yohei Maeshima, Kunihiro Fukushima, Kazunori Nishizaki, Hirofumi Akagi, Yoshiki Narimatsu, Hisashi Narimatsu, Hirofumi Makino

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Aberrant O-glycosylation of serum and tonsillar IgA1 is one of the main pathogeneses of IgA nephropathy (IgAN). However, the synthesis of underglycosylated IgA1 in tonsils has not yet been characterized. This study examined tonsillar B lymphocytes of IgAN (n= 34) using tonsils derived from patients with chronic tonsillitis (n= 24) and sleep apnea syndrome (n= 14) as a control. Gene expression of β1,3-galactosyltransferase (β3GalT), and the core 1 β3GalT-specific molecular chaperone, Cosmc, UDP-N-acetyl-α-D-galactosamine: polypeptide N-acetylgalactosaminyl-transferase 2, were significantly decreased in tonsillar CD19-positive B lymphocytes from IgAN patients compared to control tonsillar tissues as determined by real-time RT-PCR. Tonsillar B cell β3GalT gene expression significantly correlated with estimated GFR and negatively correlated with proteinuria and histological injury score. Western blotting showed the protein expression of β3GalT in the tonsils to significantly decrease in IgAN in comparison to the controls. These data suggest the downregulation of β3GalT in tonsillar B lymphocytes to be closely associated with the clinical characteristics of IgAN.

Original languageEnglish
Pages (from-to)447-455
Number of pages9
JournalClinical Immunology
Volume136
Issue number3
DOIs
Publication statusPublished - Sep 2010

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Proteinuria
Galactosyltransferases
Immunoglobulin A
B-Lymphocytes
Kidney
Palatine Tonsil
Gene Expression
Acetylgalactosamine
Tonsillitis
Molecular Chaperones
Uridine Diphosphate
Sleep Apnea Syndromes
Transferases
Glycosylation
Real-Time Polymerase Chain Reaction
Down-Regulation
Western Blotting
Peptides
Wounds and Injuries
Serum

Keywords

  • β1,3-Galactosyltransferase;
  • β3GalT;
  • Chronic tonsillitis;
  • Cosmc;
  • Estimated GFR;
  • IgA nephropathy;
  • Pp-GalNAc-T2;
  • Proteinuria;
  • Sleep apnea syndrome
  • Tonsil;

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Differential expression of glycogenes in tonsillar B lymphocytes in association with proteinuria and renal dysfunction in IgA nephropathy. / Inoue, Tatsuyuki; Sugiyama, Hitoshi; Hiki, Yoshiyuki; Takiue, Keiichi; Morinaga, Hiroshi; Kitagawa, Masashi; Maeshima, Yohei; Fukushima, Kunihiro; Nishizaki, Kazunori; Akagi, Hirofumi; Narimatsu, Yoshiki; Narimatsu, Hisashi; Makino, Hirofumi.

In: Clinical Immunology, Vol. 136, No. 3, 09.2010, p. 447-455.

Research output: Contribution to journalArticle

Inoue, Tatsuyuki ; Sugiyama, Hitoshi ; Hiki, Yoshiyuki ; Takiue, Keiichi ; Morinaga, Hiroshi ; Kitagawa, Masashi ; Maeshima, Yohei ; Fukushima, Kunihiro ; Nishizaki, Kazunori ; Akagi, Hirofumi ; Narimatsu, Yoshiki ; Narimatsu, Hisashi ; Makino, Hirofumi. / Differential expression of glycogenes in tonsillar B lymphocytes in association with proteinuria and renal dysfunction in IgA nephropathy. In: Clinical Immunology. 2010 ; Vol. 136, No. 3. pp. 447-455.
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