Differential expression of α1(IV), α2(IV), α5(IV) and α6(IV) collagen chains in the basement membrane of basal cell carcinoma

Keiko Tanaka, Ken Ichi Iyama, Kitaoka Mitsuhiko, Yoshifumi Ninomiya, Toshitaka Oohashi, Yoshikazu Sado, Tomomichi Ono

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Type IV collagen, the major component of basement membrane, consists primarily of α1(IV) and α2(IV) chains. Recently, other types of collagen IV chains, i.e. α3(IV), α4(IV), α5(IV) and α6(IV) chains, have been identified by protein chemistry and molecular cloning. We have examined the diversity of the assembly of α(IV) chains of the basement membrane surrounding turnout nests of basal cell carcinomas, in tissues from 11 patients, by immunohistochemical analysis using specific monoclonal antibodies to six α(IV) chain. The immunostaining profile of each chain differed with respect to the histological subtypes of basal cell carcinoma. In the morphea-like subtype, which was more invasive, α1(IV) and α2(IV) chains were discontinuously stained, and α5(IV) and α6(IV) chains were entirely absent. However, in the superficial subtype, which was non- aggressive, α1(IV), α2(IV), α5(IV) and α6(IV) chains were well stained compared with the other subtypes of basal cell carcinoma. In addition, in the solid subtype, which showed slow growth and ulceration, α1(IV) and α2(IV) chains were continuously stained, and α5(IV) and α6(IV) chains were discontinuous or absent. The assembly of α5(IV) and α6(IV) chains into the basement membrane was inhibited in the solid and morphea subtypes of BCC. This differential expression of type IV collagen chains seems to be associated with the invasive potential of basal cell carcinoma.

Original languageEnglish
Pages (from-to)563-570
Number of pages8
JournalHistochemical Journal
Volume29
Issue number7
DOIs
Publication statusPublished - 1997

Fingerprint

Basal Cell Carcinoma
Basement Membrane
Collagen
Collagen Type I
Localized Scleroderma
Molecular Cloning
Monoclonal Antibodies
Growth
Proteins

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Differential expression of α1(IV), α2(IV), α5(IV) and α6(IV) collagen chains in the basement membrane of basal cell carcinoma. / Tanaka, Keiko; Iyama, Ken Ichi; Mitsuhiko, Kitaoka; Ninomiya, Yoshifumi; Oohashi, Toshitaka; Sado, Yoshikazu; Ono, Tomomichi.

In: Histochemical Journal, Vol. 29, No. 7, 1997, p. 563-570.

Research output: Contribution to journalArticle

Tanaka, Keiko ; Iyama, Ken Ichi ; Mitsuhiko, Kitaoka ; Ninomiya, Yoshifumi ; Oohashi, Toshitaka ; Sado, Yoshikazu ; Ono, Tomomichi. / Differential expression of α1(IV), α2(IV), α5(IV) and α6(IV) collagen chains in the basement membrane of basal cell carcinoma. In: Histochemical Journal. 1997 ; Vol. 29, No. 7. pp. 563-570.
@article{f16962ad1c7a4cdf955c44aa47a150e2,
title = "Differential expression of α1(IV), α2(IV), α5(IV) and α6(IV) collagen chains in the basement membrane of basal cell carcinoma",
abstract = "Type IV collagen, the major component of basement membrane, consists primarily of α1(IV) and α2(IV) chains. Recently, other types of collagen IV chains, i.e. α3(IV), α4(IV), α5(IV) and α6(IV) chains, have been identified by protein chemistry and molecular cloning. We have examined the diversity of the assembly of α(IV) chains of the basement membrane surrounding turnout nests of basal cell carcinomas, in tissues from 11 patients, by immunohistochemical analysis using specific monoclonal antibodies to six α(IV) chain. The immunostaining profile of each chain differed with respect to the histological subtypes of basal cell carcinoma. In the morphea-like subtype, which was more invasive, α1(IV) and α2(IV) chains were discontinuously stained, and α5(IV) and α6(IV) chains were entirely absent. However, in the superficial subtype, which was non- aggressive, α1(IV), α2(IV), α5(IV) and α6(IV) chains were well stained compared with the other subtypes of basal cell carcinoma. In addition, in the solid subtype, which showed slow growth and ulceration, α1(IV) and α2(IV) chains were continuously stained, and α5(IV) and α6(IV) chains were discontinuous or absent. The assembly of α5(IV) and α6(IV) chains into the basement membrane was inhibited in the solid and morphea subtypes of BCC. This differential expression of type IV collagen chains seems to be associated with the invasive potential of basal cell carcinoma.",
author = "Keiko Tanaka and Iyama, {Ken Ichi} and Kitaoka Mitsuhiko and Yoshifumi Ninomiya and Toshitaka Oohashi and Yoshikazu Sado and Tomomichi Ono",
year = "1997",
doi = "10.1023/A:1026428010104",
language = "English",
volume = "29",
pages = "563--570",
journal = "Journal of Molecular Histology",
issn = "1567-2379",
publisher = "Springer Netherlands",
number = "7",

}

TY - JOUR

T1 - Differential expression of α1(IV), α2(IV), α5(IV) and α6(IV) collagen chains in the basement membrane of basal cell carcinoma

AU - Tanaka, Keiko

AU - Iyama, Ken Ichi

AU - Mitsuhiko, Kitaoka

AU - Ninomiya, Yoshifumi

AU - Oohashi, Toshitaka

AU - Sado, Yoshikazu

AU - Ono, Tomomichi

PY - 1997

Y1 - 1997

N2 - Type IV collagen, the major component of basement membrane, consists primarily of α1(IV) and α2(IV) chains. Recently, other types of collagen IV chains, i.e. α3(IV), α4(IV), α5(IV) and α6(IV) chains, have been identified by protein chemistry and molecular cloning. We have examined the diversity of the assembly of α(IV) chains of the basement membrane surrounding turnout nests of basal cell carcinomas, in tissues from 11 patients, by immunohistochemical analysis using specific monoclonal antibodies to six α(IV) chain. The immunostaining profile of each chain differed with respect to the histological subtypes of basal cell carcinoma. In the morphea-like subtype, which was more invasive, α1(IV) and α2(IV) chains were discontinuously stained, and α5(IV) and α6(IV) chains were entirely absent. However, in the superficial subtype, which was non- aggressive, α1(IV), α2(IV), α5(IV) and α6(IV) chains were well stained compared with the other subtypes of basal cell carcinoma. In addition, in the solid subtype, which showed slow growth and ulceration, α1(IV) and α2(IV) chains were continuously stained, and α5(IV) and α6(IV) chains were discontinuous or absent. The assembly of α5(IV) and α6(IV) chains into the basement membrane was inhibited in the solid and morphea subtypes of BCC. This differential expression of type IV collagen chains seems to be associated with the invasive potential of basal cell carcinoma.

AB - Type IV collagen, the major component of basement membrane, consists primarily of α1(IV) and α2(IV) chains. Recently, other types of collagen IV chains, i.e. α3(IV), α4(IV), α5(IV) and α6(IV) chains, have been identified by protein chemistry and molecular cloning. We have examined the diversity of the assembly of α(IV) chains of the basement membrane surrounding turnout nests of basal cell carcinomas, in tissues from 11 patients, by immunohistochemical analysis using specific monoclonal antibodies to six α(IV) chain. The immunostaining profile of each chain differed with respect to the histological subtypes of basal cell carcinoma. In the morphea-like subtype, which was more invasive, α1(IV) and α2(IV) chains were discontinuously stained, and α5(IV) and α6(IV) chains were entirely absent. However, in the superficial subtype, which was non- aggressive, α1(IV), α2(IV), α5(IV) and α6(IV) chains were well stained compared with the other subtypes of basal cell carcinoma. In addition, in the solid subtype, which showed slow growth and ulceration, α1(IV) and α2(IV) chains were continuously stained, and α5(IV) and α6(IV) chains were discontinuous or absent. The assembly of α5(IV) and α6(IV) chains into the basement membrane was inhibited in the solid and morphea subtypes of BCC. This differential expression of type IV collagen chains seems to be associated with the invasive potential of basal cell carcinoma.

UR - http://www.scopus.com/inward/record.url?scp=0030763737&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030763737&partnerID=8YFLogxK

U2 - 10.1023/A:1026428010104

DO - 10.1023/A:1026428010104

M3 - Article

VL - 29

SP - 563

EP - 570

JO - Journal of Molecular Histology

JF - Journal of Molecular Histology

SN - 1567-2379

IS - 7

ER -