Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, l-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening

Akira Nakatsuka, Shiho Murachi, Hironori Okunishi, Shinjiro Shiomi, Ryohei Nakano, Yasutaka Kubo, Akitsugu Inaba

Research output: Contribution to journalArticle

320 Citations (Scopus)

Abstract

We investigated the feedback regulation of ethylene biosynthesis in tomato (Lycopersicon esculentum) fruit with respect to the transition from system 1 to system 2 ethylene production. The abundance of LE-ACS2, LE-ACS4, and NR mRNAs increased in the ripening fruit concomitant with a burst in ethylene production. These increases in mRNAs with ripening were prevented to a large extent by treatment with 1-methylcyclopropene (MCP), an ethylene action inhibitor. Transcripts for the LE-ACS6 gene, which accumulated in preclimacteric fruit but not in untreated ripening fruit, did accumulate in ripening fruit treated with MCP. Treatment of young fruit with propylene prevented the accumulation of transcripts for this gene. LE-ACS1A, LE-ACS3, and TAE1 genes were expressed constitutively in the fruit throughout development and ripening irrespective of whether the fruit was treated with MCP or propylene. The transcripts for LE-ACO1 and LE-ACO4 genes already existed in preclimacteric fruit and increased greatly when ripening commenced. These increases in LE-ACO mRNA with ripening were also prevented by treatment with MCP. The results suggest that in tomato fruit the preclimacteric system 1 ethylene is possibly mediated via constitutively expressed LE-ACS1A and LE-ACS3 and negatively feedback-regulated LE-ACS6 genes with preexisting LE-ACO1 and LE-ACO4 mRNAs. At the onset of the climacteric stage, it shifts to system 2 ethylene, with a large accumulation of LE-ACS2, LE-ACS4, LE-ACO1, and LE-ACO4 mRNAs as a result of a positive feedback regulation. This transition from system 1 to system 2 ethylene production might be related to the accumulated level of NR mRNA.

Original languageEnglish
Pages (from-to)1295-1305
Number of pages11
JournalPlant Physiology
Volume118
Issue number4
Publication statusPublished - 1998

Fingerprint

1-aminocyclopropanecarboxylate synthase
1-aminocyclopropane-1-carboxylate synthase
Lycopersicon esculentum
fruiting
ethylene
Fruit
Oxidoreductases
ripening
tomatoes
receptors
fruits
1-methylcyclopropene
Genes
ethylene production
genes
Messenger RNA
propylene
Solanum lycopersicum var. lycopersicum

ASJC Scopus subject areas

  • Plant Science

Cite this

Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, l-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening. / Nakatsuka, Akira; Murachi, Shiho; Okunishi, Hironori; Shiomi, Shinjiro; Nakano, Ryohei; Kubo, Yasutaka; Inaba, Akitsugu.

In: Plant Physiology, Vol. 118, No. 4, 1998, p. 1295-1305.

Research output: Contribution to journalArticle

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AU - Murachi, Shiho

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AU - Kubo, Yasutaka

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