Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response

Fumiko Taguchi, Rena Shimizu, Rie Nakajima, Kazuhiro Toyoda, Tomonori Shiraishi, Yuki Ichinose

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

To investigate the factor that determines incompatible interactions between Pseudomonas syringae pv. tabaci and non-host plants, an elicitor of hypersensitive reaction (HR) was partially purified from the supernatant of a nutrient-poor medium of bacterial culture by DEAE column chromatography. The major protein in the elicitor-active fractions was identified as a flagellin which is a component of flagellar filaments. The flagellins purified from P. syringae pv. tomato and glycinea, incompatible pathogens of tobacco plants, induced fragmentation of chromosomal DNA and oxidative burst accompanied by programmed cell death in tobacco (Nicotiana tabacum) Bright Yellow (BY-2) cells, but the flagellin from pv. tabaci, a compatible pathogen, did not. However, the amino acid sequences of flagellins deduced from fliC genes showed a high homology among these P. syringae pathovars. In particular, the amino acid sequences of pv. tabaci and glycinea are completely identical. However, both recombinant flagellins produced in Escherichia coli possess HR-inducing activity in BY-2 cells. These results indicate that the post-translational modification of flagellins has an important role for HR-inducing ability in tobacco cells. Furthermore, we discuss the cause of a different elicitor activity among flagellins on tobacco cells and the role of flagellins in the determining specificity.

Original languageEnglish
Pages (from-to)165-174
Number of pages10
JournalPlant Physiology and Biochemistry
Volume41
Issue number2
DOIs
Publication statusPublished - Feb 1 2003

Fingerprint

Pseudomonas syringae pv. tabaci
Pseudomonas syringae
Flagellin
flagellin
Lycopersicon esculentum
tomatoes
Tobacco
hypersensitive response
tobacco
Pathogens
Amino Acid Sequence
Pseudomonas syringae pv. glycinea
amino acid sequences
cells
Pseudomonas syringae pv. tomato
Amino Acids
Column chromatography
Respiratory Burst
pathogens
post-translational modification

Keywords

  • Cell death
  • Elicitor
  • Flagellin
  • FliC
  • Hypersensitive reaction
  • Pseudomonas syringae
  • Tobacco

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry
  • Biotechnology

Cite this

Differential effects of flagellins from Pseudomonas syringae pv. tabaci, tomato and glycinea on plant defense response. / Taguchi, Fumiko; Shimizu, Rena; Nakajima, Rie; Toyoda, Kazuhiro; Shiraishi, Tomonori; Ichinose, Yuki.

In: Plant Physiology and Biochemistry, Vol. 41, No. 2, 01.02.2003, p. 165-174.

Research output: Contribution to journalArticle

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