Differential effects of dendritic cell transfer on airway hyperresponsiveness and inflammation

Toshiyuki Koya, Hiroyuki Matsuda, Shigeki Matsubara, Nobuaki Miyahara, Azzeddine Dakhama, Katsuyuki Takeda, Erwin W. Gelfand

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Dendritic cells (DCs) are considered to be the most efficient antigen presenting cells. Intratracheal administration of allergen-pulsed bone marrow-derived dendritic cells (BMDCs) before allergen challenge induces airway hyperresponsiveness (AHR) and inflammation. Ovalbumin (OVA)-pulsed BMDCs from wild-type (WT) mice were transferred into naive WT, CD4-/-, CD8 -/-, or IL-13-/- mice. Two days (short protocol) or 10 days (long protocol) after BMDC transfer,micewere challengedwith 1%OVA for 3 days and assayed 2 days later. Transfer of OVA-primed BMDCs into BALB/c or C57BL/ 6 mice elicited AHR in both protocols. Airway eosinophilia, Th2 cytokines, or goblet cell metaplasia were increased in the long but not short protocol. Lung T cells from both protocols produced Th2 cytokines in response to OVA in vitro. Carboxyfluorescein diacetate succinimidylester-labeled BMDCs were observed in bronchoalveolar lavage (BAL) fluid and lung parenchyma at early time points, andwere detected in draining lymph nodes 48 hours after transfer. CD8-/- mice developed AHR comparable to WT mice in the short protocol, but decreased levels of AHR, airway eosinophilia, Th2 cytokines in BAL fluid, and goblet cell metaplasia compared with WT mice in the long protocol. CD4-/- or IL-13-/- mice did not develop AHR or airway inflammation in either protocol. These data suggest that allergen-pulsed BMDCs initiate development of AHR that is dependent initially on CD4+ T cells, and at later time periods on CD8+ T cells and IL-13. Thus, the timing of allergen challenge after transfer of allergen-pulsed BMDC affects the development of AHR and airway inflammation.

Original languageEnglish
Pages (from-to)271-280
Number of pages10
JournalAmerican journal of respiratory cell and molecular biology
Volume41
Issue number3
DOIs
Publication statusPublished - Sep 1 2009

Keywords

  • Airway hyperresponsiveness
  • CD8 T cells
  • Dendritic cells

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

Fingerprint Dive into the research topics of 'Differential effects of dendritic cell transfer on airway hyperresponsiveness and inflammation'. Together they form a unique fingerprint.

  • Cite this