Differential display of skin mRNAs regulated under varying environmental conditions in a mudskipper

Tatsuya Sakamoto, H. Yasunaga, S. Yokota, M. Ando

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To understand the molecular mechanisms underlying the terrestrial adaptation, as well as adaptation to different salinities, of the euryhaline and amphibious mudskipper (Periophthalmus modestus), we have looked for the skin mRNAs that change during varying environmental conditions. Using differential mRNA display polymerase chain reaction, we compared skin mRNAs in mudskipper transferred from isotonic 30% seawater to fresh water or to seawater for 1 day and 7 days, as well as those kept out of water for 1 day. At the end of these periods, poly(A+)RNA was prepared from the Cl--secreting pectoral skins and also from the outer opercular skins where ion transport is negligible, and analyzed by differential display. We identified four cDNA products expressed differently under various environments as homologues of known genes. A further 34 cDNAs were expressed differentially, but they have no significant homology to identified sequences in GenBank. Northern blots demonstrate that mRNA levels of the actin-binding protein and the platelet-activating factor acetylhydrolase increased in the pectoral skins during seawater acclimation. The mRNA of the 90 kDa heat shock protein was down-regulated in water-deprived and freshwater fish, whose plasma cortisol levels were high. The aldolase mRNA was induced in both skins after desiccation. These four genes may be involved in the environmental adaptations.

Original languageEnglish
Pages (from-to)447-453
Number of pages7
JournalJournal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
Volume172
Issue number5
DOIs
Publication statusPublished - 2002
Externally publishedYes

Fingerprint

Gene Expression Profiling
skin (animal)
skin
Skin
Display devices
environmental conditions
Messenger RNA
environmental factors
Seawater
seawater
Fresh Water
microfilament proteins
Complementary DNA
Water
HSP90 Heat-Shock Proteins
Genes
Microfilament Proteins
Desiccation
fructose-bisphosphate aldolase
platelet-activating factor

Keywords

  • Amphibious mudskipper
  • Differential mRNA display polymerase chain reaction
  • Epithelium
  • Ion transport
  • Osmoregulation

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Environmental Science(all)
  • Physiology
  • Physiology (medical)

Cite this

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abstract = "To understand the molecular mechanisms underlying the terrestrial adaptation, as well as adaptation to different salinities, of the euryhaline and amphibious mudskipper (Periophthalmus modestus), we have looked for the skin mRNAs that change during varying environmental conditions. Using differential mRNA display polymerase chain reaction, we compared skin mRNAs in mudskipper transferred from isotonic 30{\%} seawater to fresh water or to seawater for 1 day and 7 days, as well as those kept out of water for 1 day. At the end of these periods, poly(A+)RNA was prepared from the Cl--secreting pectoral skins and also from the outer opercular skins where ion transport is negligible, and analyzed by differential display. We identified four cDNA products expressed differently under various environments as homologues of known genes. A further 34 cDNAs were expressed differentially, but they have no significant homology to identified sequences in GenBank. Northern blots demonstrate that mRNA levels of the actin-binding protein and the platelet-activating factor acetylhydrolase increased in the pectoral skins during seawater acclimation. The mRNA of the 90 kDa heat shock protein was down-regulated in water-deprived and freshwater fish, whose plasma cortisol levels were high. The aldolase mRNA was induced in both skins after desiccation. These four genes may be involved in the environmental adaptations.",
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AU - Sakamoto, Tatsuya

AU - Yasunaga, H.

AU - Yokota, S.

AU - Ando, M.

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