Differential changes in axonal conduction following CNS demyelination in two mouse models

Yoshio Bando, Kaoru Takakusaki, Shinji Ito, Ryuji Terayama, Makoto Kashiwayanagi, Shigetaka Yoshida

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Transgenic and disease model mice have been used to investigate the molecular mechanisms of demyelinating diseases. However, less attention has been given to elucidating changes in nerve conduction in these mice. We established an experimental system to measure the response latency of cortical neurons and examined changes in nerve conduction in cuprizone-induced demyelinating mice and in myelin basic protein-deficient shiverer mice. Stimulating and recording electrodes were placed in the right and left sensori-motor cortices, respectively. Electrical stimulation of the right cortex evoked antidromic responses in left cortical neurons with a latency of 9.38 ± 0.31 ms (n = 107; mean ± SEM). While response latency was longer in mice at 7 days and 4 weeks of cuprizone treatment (12.35 ± 0.35 ms, n = 102; 11.72 ± 0.29 ms, n = 103, respectively), response latency at 7 days and 4 weeks after removal of cuprizone was partially restored (10.72 ± 0.45 ms, n = 106; 10.27 ± 0.34 ms, n = 107, respectively). Likewise, electron microscopy showed cuprizone-induced demyelination in the corpus callosum and nearly complete remyelination after cuprizone removal. We also examined whether the myelin abnormalities in shiverer mice affected their response latencies. But there were no significant differences in response latencies in shiverer (9.83 ± 0.24 ms, n = 103) and wild-type (9.33 ± 0.22 ms, n = 112) mice. The results of these electrophysiological assessments imply that different demyelinating mechanisms, differentially affecting axon conduction, are present in the cuprizone-treated and shiverer mice, and may provide new insights to understanding the pathophysiology of demyelination in animal models in the CNS.

Original languageEnglish
Pages (from-to)1731-1742
Number of pages12
JournalEuropean Journal of Neuroscience
Volume28
Issue number9
DOIs
Publication statusPublished - Nov 2008

Fingerprint

Cuprizone
Demyelinating Diseases
Reaction Time
Neural Conduction
Neurons
Corpus Callosum
Motor Cortex
Myelin Sheath
Electric Stimulation
Axons
Electron Microscopy
Electrodes
Animal Models

Keywords

  • Myelin
  • Nerve conduction
  • Oligodendrocytes

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Bando, Y., Takakusaki, K., Ito, S., Terayama, R., Kashiwayanagi, M., & Yoshida, S. (2008). Differential changes in axonal conduction following CNS demyelination in two mouse models. European Journal of Neuroscience, 28(9), 1731-1742. https://doi.org/10.1111/j.1460-9568.2008.06474.x

Differential changes in axonal conduction following CNS demyelination in two mouse models. / Bando, Yoshio; Takakusaki, Kaoru; Ito, Shinji; Terayama, Ryuji; Kashiwayanagi, Makoto; Yoshida, Shigetaka.

In: European Journal of Neuroscience, Vol. 28, No. 9, 11.2008, p. 1731-1742.

Research output: Contribution to journalArticle

Bando, Y, Takakusaki, K, Ito, S, Terayama, R, Kashiwayanagi, M & Yoshida, S 2008, 'Differential changes in axonal conduction following CNS demyelination in two mouse models', European Journal of Neuroscience, vol. 28, no. 9, pp. 1731-1742. https://doi.org/10.1111/j.1460-9568.2008.06474.x
Bando, Yoshio ; Takakusaki, Kaoru ; Ito, Shinji ; Terayama, Ryuji ; Kashiwayanagi, Makoto ; Yoshida, Shigetaka. / Differential changes in axonal conduction following CNS demyelination in two mouse models. In: European Journal of Neuroscience. 2008 ; Vol. 28, No. 9. pp. 1731-1742.
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