Differential Al resistance and citrate secretion in barley (Hordeum vulgare L.)

Zhuqing Zhao, Jian Feng Ma, Kazuhiro Sato, Kazuyoshi Takeda

Research output: Contribution to journalArticle

92 Citations (Scopus)

Abstract

While barley (Hordeum vulgare L.) is the most sensitive species to Al toxicity among small-grain crops, variation in Al resistance between cultivars does exist. We examined the mechanism responsible for differential Al resistance in 21 barley varieties. Citrate was secreted from the roots in response to Al stress. A positive correlation between citrate secretion and Al resistance [(root elongation with Al)/(root elongation without Al)] and a negative correlation between citrate secretion and Al content of root apices, were obtained, suggesting that citrate secretion from the root apices plays an important role in excluding Al and thereby detoxifying Al. The Al-induced secretion of citrate was characterized using an Al-resistant variety (Sigurdkorn) and an Al-sensitive variety (Kearney). In Sigurdkorn, Al-induced secretion of citrate occurred within 20 min, and the secretion did not increase with increasing external Al concentration. The Al-induced citrate secretion ceased at low temperature (6 °C) and was inhibited by anion-channel inhibitors. Internal citrate content of root apices was increased by Al exposure in Sigurdkorn, but was not affected in Kearney. The activity of citrate synthase was unaffected by Al in both Al-resistant and Al-sensitive varieties. The secretion rate of organic acid anions from barley was the lowest among wheat, rye and triticale.

Original languageEnglish
Pages (from-to)794-800
Number of pages7
JournalPlanta
Volume217
Issue number5
DOIs
Publication statusPublished - Sep 1 2003

Fingerprint

Hordeum
Citric Acid
citrates
Hordeum vulgare
barley
secretion
anions
Anions
root growth
Citrate (si)-Synthase
citrate (si)-synthase
triticale
grain crops
rye
Triticum
organic acids and salts
toxicity
wheat
Temperature
Acids

Keywords

  • Aluminium
  • Citrate
  • Hordeum
  • Metabolism
  • Secretion

ASJC Scopus subject areas

  • Plant Science

Cite this

Differential Al resistance and citrate secretion in barley (Hordeum vulgare L.). / Zhao, Zhuqing; Ma, Jian Feng; Sato, Kazuhiro; Takeda, Kazuyoshi.

In: Planta, Vol. 217, No. 5, 01.09.2003, p. 794-800.

Research output: Contribution to journalArticle

Zhao, Zhuqing ; Ma, Jian Feng ; Sato, Kazuhiro ; Takeda, Kazuyoshi. / Differential Al resistance and citrate secretion in barley (Hordeum vulgare L.). In: Planta. 2003 ; Vol. 217, No. 5. pp. 794-800.
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