Different type and localization of CD44 on surface membrane of regenerative renal tubular epithelial cells in vivo

Yuansheng Xie, Shinichi Nishi, Sachiko Fukase, Hiroaki Nakamura, Xiangmei Chen, Naofumi Imaia, Minoru Sakatsume, Akihiko Saito, Mitsuhiro Ueno, Ichiei Narita, Toshio Yamamoto, Fumitake Gejyo

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: CD44 is a transmembrane glycoprotein comprising an extracellular domain, a transmembrane domain, and a cytoplasmic tail. Previous studies demonstrated that CD44 was generally restricted to lateral-basal plasma membrane (PM) of epithelial cells, whether it localized on apical PM in vivo has not been clarified. Methods: In this study, we used a gentamicin-induced acute tubular necrosis (ATN) and spontaneous recovery model in rats and two distinct antibodies, an anti-rat distal extracellular domain (OX49) of standard CD44 (CD44-OX49) and an anti-rat CD44 cytoplasmic tail (CD44CPT), to survey the localization of CD44-OX49 and CD44CPT on the PM in renal tubular epithelial cells in different recovery stages after ATN with immunohistochemistry and immunoelectron-microscopic examinations. Results: CD44-OX49 was localized not only on the lateral-basal PM in tubular epithelial cells, but also on the apical surface membrane in PCNA-positive newly regenerative tubular epithelial cells in early recovery stages after ATN. However, CD44CPT was only localized on the lateral-basal PM. The immunoelectron-microscopic results showed that CD44-OX49 localization was changed from the apical to lateral to basal surface membrane in renal tubular epithelial cells during the recovery process after ATN, finally disappearing from basal PM when normal polarized epithelial cells formed. Conclusions: These results suggest that there were two types of CD44 including CD44 without a cytoplasmic tail localizing on the apical surface membrane related to newly regenerative epithelial cells, and CD44 with a cytoplasmic tail localizing on the lateral-basal PM related to establishment of tubular epithelial cell polarity after ATN in vivo.

Original languageEnglish
Pages (from-to)188-197
Number of pages10
JournalAmerican Journal of Nephrology
Volume24
Issue number2
DOIs
Publication statusPublished - 2004

Fingerprint

Epithelial Cells
Kidney
Tail
Cell Membrane
Membranes
Necrosis
Cell Polarity
Proliferating Cell Nuclear Antigen
Gentamicins
Anti-Idiotypic Antibodies
Glycoproteins
Immunohistochemistry

Keywords

  • Acute tubular necrosis
  • CD44
  • Plasma membrane

ASJC Scopus subject areas

  • Nephrology

Cite this

Different type and localization of CD44 on surface membrane of regenerative renal tubular epithelial cells in vivo. / Xie, Yuansheng; Nishi, Shinichi; Fukase, Sachiko; Nakamura, Hiroaki; Chen, Xiangmei; Imaia, Naofumi; Sakatsume, Minoru; Saito, Akihiko; Ueno, Mitsuhiro; Narita, Ichiei; Yamamoto, Toshio; Gejyo, Fumitake.

In: American Journal of Nephrology, Vol. 24, No. 2, 2004, p. 188-197.

Research output: Contribution to journalArticle

Xie, Y, Nishi, S, Fukase, S, Nakamura, H, Chen, X, Imaia, N, Sakatsume, M, Saito, A, Ueno, M, Narita, I, Yamamoto, T & Gejyo, F 2004, 'Different type and localization of CD44 on surface membrane of regenerative renal tubular epithelial cells in vivo', American Journal of Nephrology, vol. 24, no. 2, pp. 188-197. https://doi.org/10.1159/000076758
Xie, Yuansheng ; Nishi, Shinichi ; Fukase, Sachiko ; Nakamura, Hiroaki ; Chen, Xiangmei ; Imaia, Naofumi ; Sakatsume, Minoru ; Saito, Akihiko ; Ueno, Mitsuhiro ; Narita, Ichiei ; Yamamoto, Toshio ; Gejyo, Fumitake. / Different type and localization of CD44 on surface membrane of regenerative renal tubular epithelial cells in vivo. In: American Journal of Nephrology. 2004 ; Vol. 24, No. 2. pp. 188-197.
@article{f4b1af97d4f2465a86fa3dcdcc64003a,
title = "Different type and localization of CD44 on surface membrane of regenerative renal tubular epithelial cells in vivo",
abstract = "Background: CD44 is a transmembrane glycoprotein comprising an extracellular domain, a transmembrane domain, and a cytoplasmic tail. Previous studies demonstrated that CD44 was generally restricted to lateral-basal plasma membrane (PM) of epithelial cells, whether it localized on apical PM in vivo has not been clarified. Methods: In this study, we used a gentamicin-induced acute tubular necrosis (ATN) and spontaneous recovery model in rats and two distinct antibodies, an anti-rat distal extracellular domain (OX49) of standard CD44 (CD44-OX49) and an anti-rat CD44 cytoplasmic tail (CD44CPT), to survey the localization of CD44-OX49 and CD44CPT on the PM in renal tubular epithelial cells in different recovery stages after ATN with immunohistochemistry and immunoelectron-microscopic examinations. Results: CD44-OX49 was localized not only on the lateral-basal PM in tubular epithelial cells, but also on the apical surface membrane in PCNA-positive newly regenerative tubular epithelial cells in early recovery stages after ATN. However, CD44CPT was only localized on the lateral-basal PM. The immunoelectron-microscopic results showed that CD44-OX49 localization was changed from the apical to lateral to basal surface membrane in renal tubular epithelial cells during the recovery process after ATN, finally disappearing from basal PM when normal polarized epithelial cells formed. Conclusions: These results suggest that there were two types of CD44 including CD44 without a cytoplasmic tail localizing on the apical surface membrane related to newly regenerative epithelial cells, and CD44 with a cytoplasmic tail localizing on the lateral-basal PM related to establishment of tubular epithelial cell polarity after ATN in vivo.",
keywords = "Acute tubular necrosis, CD44, Plasma membrane",
author = "Yuansheng Xie and Shinichi Nishi and Sachiko Fukase and Hiroaki Nakamura and Xiangmei Chen and Naofumi Imaia and Minoru Sakatsume and Akihiko Saito and Mitsuhiro Ueno and Ichiei Narita and Toshio Yamamoto and Fumitake Gejyo",
year = "2004",
doi = "10.1159/000076758",
language = "English",
volume = "24",
pages = "188--197",
journal = "American Journal of Nephrology",
issn = "0250-8095",
publisher = "S. Karger AG",
number = "2",

}

TY - JOUR

T1 - Different type and localization of CD44 on surface membrane of regenerative renal tubular epithelial cells in vivo

AU - Xie, Yuansheng

AU - Nishi, Shinichi

AU - Fukase, Sachiko

AU - Nakamura, Hiroaki

AU - Chen, Xiangmei

AU - Imaia, Naofumi

AU - Sakatsume, Minoru

AU - Saito, Akihiko

AU - Ueno, Mitsuhiro

AU - Narita, Ichiei

AU - Yamamoto, Toshio

AU - Gejyo, Fumitake

PY - 2004

Y1 - 2004

N2 - Background: CD44 is a transmembrane glycoprotein comprising an extracellular domain, a transmembrane domain, and a cytoplasmic tail. Previous studies demonstrated that CD44 was generally restricted to lateral-basal plasma membrane (PM) of epithelial cells, whether it localized on apical PM in vivo has not been clarified. Methods: In this study, we used a gentamicin-induced acute tubular necrosis (ATN) and spontaneous recovery model in rats and two distinct antibodies, an anti-rat distal extracellular domain (OX49) of standard CD44 (CD44-OX49) and an anti-rat CD44 cytoplasmic tail (CD44CPT), to survey the localization of CD44-OX49 and CD44CPT on the PM in renal tubular epithelial cells in different recovery stages after ATN with immunohistochemistry and immunoelectron-microscopic examinations. Results: CD44-OX49 was localized not only on the lateral-basal PM in tubular epithelial cells, but also on the apical surface membrane in PCNA-positive newly regenerative tubular epithelial cells in early recovery stages after ATN. However, CD44CPT was only localized on the lateral-basal PM. The immunoelectron-microscopic results showed that CD44-OX49 localization was changed from the apical to lateral to basal surface membrane in renal tubular epithelial cells during the recovery process after ATN, finally disappearing from basal PM when normal polarized epithelial cells formed. Conclusions: These results suggest that there were two types of CD44 including CD44 without a cytoplasmic tail localizing on the apical surface membrane related to newly regenerative epithelial cells, and CD44 with a cytoplasmic tail localizing on the lateral-basal PM related to establishment of tubular epithelial cell polarity after ATN in vivo.

AB - Background: CD44 is a transmembrane glycoprotein comprising an extracellular domain, a transmembrane domain, and a cytoplasmic tail. Previous studies demonstrated that CD44 was generally restricted to lateral-basal plasma membrane (PM) of epithelial cells, whether it localized on apical PM in vivo has not been clarified. Methods: In this study, we used a gentamicin-induced acute tubular necrosis (ATN) and spontaneous recovery model in rats and two distinct antibodies, an anti-rat distal extracellular domain (OX49) of standard CD44 (CD44-OX49) and an anti-rat CD44 cytoplasmic tail (CD44CPT), to survey the localization of CD44-OX49 and CD44CPT on the PM in renal tubular epithelial cells in different recovery stages after ATN with immunohistochemistry and immunoelectron-microscopic examinations. Results: CD44-OX49 was localized not only on the lateral-basal PM in tubular epithelial cells, but also on the apical surface membrane in PCNA-positive newly regenerative tubular epithelial cells in early recovery stages after ATN. However, CD44CPT was only localized on the lateral-basal PM. The immunoelectron-microscopic results showed that CD44-OX49 localization was changed from the apical to lateral to basal surface membrane in renal tubular epithelial cells during the recovery process after ATN, finally disappearing from basal PM when normal polarized epithelial cells formed. Conclusions: These results suggest that there were two types of CD44 including CD44 without a cytoplasmic tail localizing on the apical surface membrane related to newly regenerative epithelial cells, and CD44 with a cytoplasmic tail localizing on the lateral-basal PM related to establishment of tubular epithelial cell polarity after ATN in vivo.

KW - Acute tubular necrosis

KW - CD44

KW - Plasma membrane

UR - http://www.scopus.com/inward/record.url?scp=11144353918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=11144353918&partnerID=8YFLogxK

U2 - 10.1159/000076758

DO - 10.1159/000076758

M3 - Article

VL - 24

SP - 188

EP - 197

JO - American Journal of Nephrology

JF - American Journal of Nephrology

SN - 0250-8095

IS - 2

ER -