Different Prostanoids Are Involved in Bradykinin-Induced Endothelium-Dependent and -Independent Vasoconstriction in Rat Mesenteric Resistance Arteries

Hideki Nawa, Yuji Kurosaki, Hiromu Kawasaki

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Mechanisms underlying bradykinin-induced vasoconstriction were investigated in rat perfused mesenteric vascular beds with active tone. In preparations with intact endothelium, bolus injections of bradykinin (1 to 1, 000 pmol) dose-dependently produced three-phase vascular effects, which consisted of a first-phase vasodilation followed by a second-phase vasoconstriction and a subsequent third-phase vasodilation; these effects were abolished by FR172357 (bradykinin B2-receptor antagonist), but not by des-Arg9-[Leu8]-bradykinin (bradykinin B 1-receptor antagonist). In preparations with intact endothelium, indomethacin (cyclooxygenase inhibitor), seratrodast (thromboxane A2 (TXA2)-receptor antagonist), ONO-3708 (TXA2/prostaglandin H2 (PGH2)-receptor antagonist) or ozagrel (TXA 2 synthesis inhibitor) markedly inhibited the bradykinin-induced vasoconstriction. In preparations without endothelium, the bradykinin-induced vasoconstriction was abolished by indomethacin and ONO-3708, while seratrodast and ozagrel had no effect. These results suggest that the endothelium-dependent vasoconstriction of bradykinin is mainly mediated by TXA2 and that prostanoids other than TXA2, probably PGH2, in mesenteric vascular smooth muscle are responsible for bradykinin-induced endothelium-independent vasoconstriction.

Original languageEnglish
Pages (from-to)115-121
Number of pages7
JournalJournal of Pharmacological Sciences
Volume94
Issue number2
DOIs
Publication statusPublished - Feb 1 2004

Keywords

  • Bradykinin
  • Prostaglandin H
  • Rat mesenteric resistance artery
  • Thromboxane A
  • Vasoconstriction

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

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