Different mechanisms of four aluminum (Al)-resistant transgenes for Al toxicity in Arabidopsis

B. Ezaki, Maki Katsuhara, M. Kawamura, H. Matsumoto

Research output: Contribution to journalArticle

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Abstract

We have characterized the mechanism of action of four transgenes (AtBCB [Arabidopsis blue copper-binding protein], parB [tobacco {Nicotiana tabacum} glutathione S-transferase], NtPox [tobacco peroxidase], and NtGDI1 [tobacco GDP dissociation inhibitor]) that independently Al resistance on transgenic Arabidopsis. All four transgenic lines showed lower deposition of callose after Al treatment than the Landsberg erecta ecotype of Arabidopsis, confirming that the four genes function to ameliorate Al toxicity. Influx and efflux experiments of Al ions suggested that the AtBCB gene may suppress Al absorption, whereas expression of the NtGDI1 gene promotes a release of Al in the root tip region of Arabidopsis. The total enzyme activities of glutathione S-transferases or peroxidases in transgenic lines carrying either the parB or NtPox genes were significantly higher than in the Landsberg erecta ecotype of Arabidopsis, and these enzyme activities were maintained at higher levels during Al stress. Furthermore, lipid peroxidation caused by Al stress was repressed in these two transgenic lines, suggesting that overexpression of these two genes diminishes oxidative damage caused by Al stress. Al-treated roots of transgenic plants were also stained by 4′,6-diamino-2-phenylindole to monitor cell death caused by Al toxicity. The result suggested that cell death is repressed in the NtPox line. Analysis of F1 hybrids between the four transgenic lines suggests that more resistant transgenic plants can be constructed by combinations of these four genes.

Original languageEnglish
Pages (from-to)918-927
Number of pages10
JournalPlant Physiology
Volume127
Issue number3
DOIs
Publication statusPublished - 2001

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Aluminum
Transgenes
Arabidopsis
transgenes
aluminum
toxicity
Tobacco
genetically modified organisms
Ecotype
tobacco
Genes
Genetically Modified Plants
Glutathione Transferase
ecotypes
glutathione transferase
genes
cell death
transgenic plants
Cell Death
Guanine Nucleotide Dissociation Inhibitors

ASJC Scopus subject areas

  • Plant Science

Cite this

Different mechanisms of four aluminum (Al)-resistant transgenes for Al toxicity in Arabidopsis. / Ezaki, B.; Katsuhara, Maki; Kawamura, M.; Matsumoto, H.

In: Plant Physiology, Vol. 127, No. 3, 2001, p. 918-927.

Research output: Contribution to journalArticle

Ezaki, B. ; Katsuhara, Maki ; Kawamura, M. ; Matsumoto, H. / Different mechanisms of four aluminum (Al)-resistant transgenes for Al toxicity in Arabidopsis. In: Plant Physiology. 2001 ; Vol. 127, No. 3. pp. 918-927.
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