Abstract
PURPOSE: To arrive at an understanding of the differences in gene and protein expression between human lateral and medial rectus muscle tissue excised from strabismic patients. METHODS: Resected fragments of the lateral and medial rectus muscles obtained during strabismus surgery were frozen under liquid nitrogen and pulverized by a Freezer/Mill system. Radiolabelled cDNA was synthesized from polyA+ RNA and hybridized with a gene array nylon membrane (Atlas Human 1.2 Array) to detect the difference in gene expression between the lateral and medial rectus muscles. Based on the gene array results, tissue inhibitor of metalloproteinases (TIMP)-1 and 2, matrix metalloproteinase (MMP)-2, and bone morphogenetic protein (BMP)-4 were measured in solubilized muscle tissues by enzyme immunoassay and normalized by unit length of resection. RESULTS: The resected lateral rectus muscle had significantly smaller amounts of TIMP-1,TIMP-2 and BMP-4 but larger amounts of MMP-2 compared to the medial rectus muscle (P <0.001, Mann-Whitney U test).The amounts of TIMP-1 in the muscle when the lateral and medial rectus were combined were positively correlated with TIMP-2 and BMP-4, while the amounts of TIMP-2 positively correlated with BMP-4 (P <0.001, Spearman rank correlation test). The amounts of BMP-4 in both muscles increased significantly with age (P = 0.0003). CONCLUSIONS: Resected tissue from the lateral and medial rectus muscles had different levels of expression of TIMP-1 and 2, MMP-2, and BMP-4. These molecular differences may underlie different characteristics of the two extraocular muscles and may also influence the process of wound healing after strabismus surgery.
| Original language | English |
|---|---|
| Pages (from-to) | 145-155 |
| Number of pages | 11 |
| Journal | Strabismus |
| Volume | 11 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sep 2003 |
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Keywords
- Bone morphogenetic protein (BMP)
- Extraocular muscle biochemistry
- Lateral rectus muscle
- Matrix metalloproteinase (MMP)
- Medial rectus muscle
- Tissue inhibitor of metalloproteinase (TIMP)
ASJC Scopus subject areas
- Ophthalmology
Cite this
Different levels of TIMPs and MMPs in human lateral and medial rectus muscle tissue excised from strabismic patients. / Kitada, Mizue; Matsuo, Toshihiko; Yamane, Takashi; Hasebe, Satoshi; Ohtsuki, Hiroshi.
In: Strabismus, Vol. 11, No. 3, 09.2003, p. 145-155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Different levels of TIMPs and MMPs in human lateral and medial rectus muscle tissue excised from strabismic patients
AU - Kitada, Mizue
AU - Matsuo, Toshihiko
AU - Yamane, Takashi
AU - Hasebe, Satoshi
AU - Ohtsuki, Hiroshi
PY - 2003/9
Y1 - 2003/9
N2 - PURPOSE: To arrive at an understanding of the differences in gene and protein expression between human lateral and medial rectus muscle tissue excised from strabismic patients. METHODS: Resected fragments of the lateral and medial rectus muscles obtained during strabismus surgery were frozen under liquid nitrogen and pulverized by a Freezer/Mill system. Radiolabelled cDNA was synthesized from polyA+ RNA and hybridized with a gene array nylon membrane (Atlas Human 1.2 Array) to detect the difference in gene expression between the lateral and medial rectus muscles. Based on the gene array results, tissue inhibitor of metalloproteinases (TIMP)-1 and 2, matrix metalloproteinase (MMP)-2, and bone morphogenetic protein (BMP)-4 were measured in solubilized muscle tissues by enzyme immunoassay and normalized by unit length of resection. RESULTS: The resected lateral rectus muscle had significantly smaller amounts of TIMP-1,TIMP-2 and BMP-4 but larger amounts of MMP-2 compared to the medial rectus muscle (P <0.001, Mann-Whitney U test).The amounts of TIMP-1 in the muscle when the lateral and medial rectus were combined were positively correlated with TIMP-2 and BMP-4, while the amounts of TIMP-2 positively correlated with BMP-4 (P <0.001, Spearman rank correlation test). The amounts of BMP-4 in both muscles increased significantly with age (P = 0.0003). CONCLUSIONS: Resected tissue from the lateral and medial rectus muscles had different levels of expression of TIMP-1 and 2, MMP-2, and BMP-4. These molecular differences may underlie different characteristics of the two extraocular muscles and may also influence the process of wound healing after strabismus surgery.
AB - PURPOSE: To arrive at an understanding of the differences in gene and protein expression between human lateral and medial rectus muscle tissue excised from strabismic patients. METHODS: Resected fragments of the lateral and medial rectus muscles obtained during strabismus surgery were frozen under liquid nitrogen and pulverized by a Freezer/Mill system. Radiolabelled cDNA was synthesized from polyA+ RNA and hybridized with a gene array nylon membrane (Atlas Human 1.2 Array) to detect the difference in gene expression between the lateral and medial rectus muscles. Based on the gene array results, tissue inhibitor of metalloproteinases (TIMP)-1 and 2, matrix metalloproteinase (MMP)-2, and bone morphogenetic protein (BMP)-4 were measured in solubilized muscle tissues by enzyme immunoassay and normalized by unit length of resection. RESULTS: The resected lateral rectus muscle had significantly smaller amounts of TIMP-1,TIMP-2 and BMP-4 but larger amounts of MMP-2 compared to the medial rectus muscle (P <0.001, Mann-Whitney U test).The amounts of TIMP-1 in the muscle when the lateral and medial rectus were combined were positively correlated with TIMP-2 and BMP-4, while the amounts of TIMP-2 positively correlated with BMP-4 (P <0.001, Spearman rank correlation test). The amounts of BMP-4 in both muscles increased significantly with age (P = 0.0003). CONCLUSIONS: Resected tissue from the lateral and medial rectus muscles had different levels of expression of TIMP-1 and 2, MMP-2, and BMP-4. These molecular differences may underlie different characteristics of the two extraocular muscles and may also influence the process of wound healing after strabismus surgery.
KW - Bone morphogenetic protein (BMP)
KW - Extraocular muscle biochemistry
KW - Lateral rectus muscle
KW - Matrix metalloproteinase (MMP)
KW - Medial rectus muscle
KW - Tissue inhibitor of metalloproteinase (TIMP)
UR - http://www.scopus.com/inward/record.url?scp=0142153862&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0142153862&partnerID=8YFLogxK
U2 - 10.1076/stra.11.3.145.16647
DO - 10.1076/stra.11.3.145.16647
M3 - Article
VL - 11
SP - 145
EP - 155
JO - Strabismus
JF - Strabismus
SN - 0927-3972
IS - 3
ER -