Different growth properties in response to epidermal growth factor and interleukin-6 of primary keratinocytes derived from normal and psoriatic lesional skin

Noritaka Oyama, Masayuki Sekimata, Yoshimichi Nihei, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Epidermal growth factor (EGF) family members and its receptor (EGFR) are thought to have an important role in the proliferation of epidermal keratinocytes. In this report, we investigated the EGF/EGFR system in primary keratinocytes derived from normal and psoriatic lesional skin. EGF elicited the growth of both normal human keratinocytes (NHKs) and psoriatic lesional keratinocytes (PLKs). Interleukin-6 (IL-6) potentiated the EGF-dependent growth of NHKs, but has no observable effect on PLKs, while IL-6 itself showed no growth-stimulating activities in both cell types. Immunodetection and in situ hybridization analyses revealed that IL-6 induces EGFR expression in NHKs in a time- and dose-dependent manner. This EGFR expression decreased reversibly to an undetectable level when IL-6-treated NHKs were re-cultured in IL-6-free conditions. On the other hand, PLKs expressed high levels of EGFR even when unstimulated and the expression level was not affected by IL- 6 stimulation. These results suggest that the EGF/EGFR system is involved in the growth of NHKs and PLKs and that IL-6 potentiates NHK growth partly through the induction of EGFR. The different EGFR regulatory system may contribute to the pathogenesis of psoriasis.

Original languageEnglish
Pages (from-to)120-128
Number of pages9
JournalJournal of Dermatological Science
Volume16
Issue number2
DOIs
Publication statusPublished - Jan 1998
Externally publishedYes

Fingerprint

Keratinocytes
Epidermal Growth Factor
Interleukin-6
Skin
Growth
Psoriasis
In Situ Hybridization

Keywords

  • Cell growth
  • Epidermal growth factor receptor
  • Interleukin-6
  • Keratinocytes
  • Psoriasis

ASJC Scopus subject areas

  • Dermatology

Cite this

Different growth properties in response to epidermal growth factor and interleukin-6 of primary keratinocytes derived from normal and psoriatic lesional skin. / Oyama, Noritaka; Sekimata, Masayuki; Nihei, Yoshimichi; Iwatsuki, Keiji; Homma, Yoshimi; Kaneko, Fumio.

In: Journal of Dermatological Science, Vol. 16, No. 2, 01.1998, p. 120-128.

Research output: Contribution to journalArticle

Oyama, Noritaka ; Sekimata, Masayuki ; Nihei, Yoshimichi ; Iwatsuki, Keiji ; Homma, Yoshimi ; Kaneko, Fumio. / Different growth properties in response to epidermal growth factor and interleukin-6 of primary keratinocytes derived from normal and psoriatic lesional skin. In: Journal of Dermatological Science. 1998 ; Vol. 16, No. 2. pp. 120-128.
@article{48c132ebe1f842e795414db1305aa0f6,
title = "Different growth properties in response to epidermal growth factor and interleukin-6 of primary keratinocytes derived from normal and psoriatic lesional skin",
abstract = "Epidermal growth factor (EGF) family members and its receptor (EGFR) are thought to have an important role in the proliferation of epidermal keratinocytes. In this report, we investigated the EGF/EGFR system in primary keratinocytes derived from normal and psoriatic lesional skin. EGF elicited the growth of both normal human keratinocytes (NHKs) and psoriatic lesional keratinocytes (PLKs). Interleukin-6 (IL-6) potentiated the EGF-dependent growth of NHKs, but has no observable effect on PLKs, while IL-6 itself showed no growth-stimulating activities in both cell types. Immunodetection and in situ hybridization analyses revealed that IL-6 induces EGFR expression in NHKs in a time- and dose-dependent manner. This EGFR expression decreased reversibly to an undetectable level when IL-6-treated NHKs were re-cultured in IL-6-free conditions. On the other hand, PLKs expressed high levels of EGFR even when unstimulated and the expression level was not affected by IL- 6 stimulation. These results suggest that the EGF/EGFR system is involved in the growth of NHKs and PLKs and that IL-6 potentiates NHK growth partly through the induction of EGFR. The different EGFR regulatory system may contribute to the pathogenesis of psoriasis.",
keywords = "Cell growth, Epidermal growth factor receptor, Interleukin-6, Keratinocytes, Psoriasis",
author = "Noritaka Oyama and Masayuki Sekimata and Yoshimichi Nihei and Keiji Iwatsuki and Yoshimi Homma and Fumio Kaneko",
year = "1998",
month = "1",
doi = "10.1016/S0923-1811(97)00040-6",
language = "English",
volume = "16",
pages = "120--128",
journal = "Journal of Dermatological Science",
issn = "0923-1811",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - Different growth properties in response to epidermal growth factor and interleukin-6 of primary keratinocytes derived from normal and psoriatic lesional skin

AU - Oyama, Noritaka

AU - Sekimata, Masayuki

AU - Nihei, Yoshimichi

AU - Iwatsuki, Keiji

AU - Homma, Yoshimi

AU - Kaneko, Fumio

PY - 1998/1

Y1 - 1998/1

N2 - Epidermal growth factor (EGF) family members and its receptor (EGFR) are thought to have an important role in the proliferation of epidermal keratinocytes. In this report, we investigated the EGF/EGFR system in primary keratinocytes derived from normal and psoriatic lesional skin. EGF elicited the growth of both normal human keratinocytes (NHKs) and psoriatic lesional keratinocytes (PLKs). Interleukin-6 (IL-6) potentiated the EGF-dependent growth of NHKs, but has no observable effect on PLKs, while IL-6 itself showed no growth-stimulating activities in both cell types. Immunodetection and in situ hybridization analyses revealed that IL-6 induces EGFR expression in NHKs in a time- and dose-dependent manner. This EGFR expression decreased reversibly to an undetectable level when IL-6-treated NHKs were re-cultured in IL-6-free conditions. On the other hand, PLKs expressed high levels of EGFR even when unstimulated and the expression level was not affected by IL- 6 stimulation. These results suggest that the EGF/EGFR system is involved in the growth of NHKs and PLKs and that IL-6 potentiates NHK growth partly through the induction of EGFR. The different EGFR regulatory system may contribute to the pathogenesis of psoriasis.

AB - Epidermal growth factor (EGF) family members and its receptor (EGFR) are thought to have an important role in the proliferation of epidermal keratinocytes. In this report, we investigated the EGF/EGFR system in primary keratinocytes derived from normal and psoriatic lesional skin. EGF elicited the growth of both normal human keratinocytes (NHKs) and psoriatic lesional keratinocytes (PLKs). Interleukin-6 (IL-6) potentiated the EGF-dependent growth of NHKs, but has no observable effect on PLKs, while IL-6 itself showed no growth-stimulating activities in both cell types. Immunodetection and in situ hybridization analyses revealed that IL-6 induces EGFR expression in NHKs in a time- and dose-dependent manner. This EGFR expression decreased reversibly to an undetectable level when IL-6-treated NHKs were re-cultured in IL-6-free conditions. On the other hand, PLKs expressed high levels of EGFR even when unstimulated and the expression level was not affected by IL- 6 stimulation. These results suggest that the EGF/EGFR system is involved in the growth of NHKs and PLKs and that IL-6 potentiates NHK growth partly through the induction of EGFR. The different EGFR regulatory system may contribute to the pathogenesis of psoriasis.

KW - Cell growth

KW - Epidermal growth factor receptor

KW - Interleukin-6

KW - Keratinocytes

KW - Psoriasis

UR - http://www.scopus.com/inward/record.url?scp=0030627442&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030627442&partnerID=8YFLogxK

U2 - 10.1016/S0923-1811(97)00040-6

DO - 10.1016/S0923-1811(97)00040-6

M3 - Article

VL - 16

SP - 120

EP - 128

JO - Journal of Dermatological Science

JF - Journal of Dermatological Science

SN - 0923-1811

IS - 2

ER -