Different effect of diastereoisomers of L-cystathionine sulfoxide on the stimulus coupled responses of human neutrophils

Yosei Shimazaki, Jianying Zhang, Hiroshi Wakiguchi, Takanobu Kurashige, Yasuhiro Sagara, Noriyoshi Masuoka, Jun Ohta, Toshihiko Ubuka, Hiroyuki Kodama

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Abstract

The priming effect of L-cystathionine sulfoxide, which is one of the unusual cystathionine metabolites found in the urine of patients with cystathioninuria, on the stimulus-induced superoxide generation by human neutrophils was examined. The synthetic L-cystathionine sulfoxide significantly enhanced the superoxide generations induced by N-formyl-methionyl-leucyl-phenylalanine [fMLP], opsonized zymosan [OZ], arachidonic acid [AA], and phorbol 12-myristate 13-acetate [PMA]. Then the synthetic L-cystathionine sulfoxide was separated into two diastereoisomers, CS-I and CS-II, which showed a peak at 76 and 83 min on chromatogram by amino acid analyzer, respectively. CS-I enhanced the superoxide generations induced by AA and PMA but not those induced by fMLP and OZ. On the contrary, CS-II enhanced the superoxide generations induced by fMLP and OZ but not those induced by AA and PMA. The superoxide generation induced by PMA with CS-I was suppressed by H-7 and was enhanced by genistein, while that by fMLP with CS-II was suppressed by genistein and was enhanced by H-7.

Original languageEnglish
Pages (from-to)387-391
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume247
Issue number2
DOIs
Publication statusPublished - Jun 18 1998

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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