Different amounts of DNA in each mitochondrion in rice root

Hideki Takanashi, Shin Ichi Arimura, Wataru Sakamoto, Nobuhiro Tsutsumi

    Research output: Contribution to journalArticlepeer-review

    14 Citations (Scopus)

    Abstract

    The DNA content of individual mitochondria in rice root cells was analyzed by fluorescence microscopy. Differences in DNA content were detected between individual mitochondria. Some mitochondria contained no detectable nucleoid (DNA-protein complexes). The percent of mitochondria with DAPI(4′,6- Diamidino-2-phenylindole) -stained nucleoids varied over the length of the root (root base, 33%; middle portion of root, 41%; root tip, 91%). The mean amounts of DNA per mitochondrial nucleoid were equivalent to 46.4 kbp in the root base, 52.0 kbp in the middle portion of root and 124.2 kbp in the root tip. The amount of DNA in individual mitochondria and the ratio of mitochondria with visible nucleoids were higher in the root tip than in other parts of the root. The estimated amount of DNA in almost all of the observed mitochondria was smaller than the amount of DNA equivalent to the rice mitochondrial genome size (490 kbp), even in root tip.

    Original languageEnglish
    Pages (from-to)215-218
    Number of pages4
    JournalGenes and Genetic Systems
    Volume81
    Issue number3
    DOIs
    Publication statusPublished - 2006

    Keywords

    • DAPI
    • DNA
    • Fluorescence microscopy
    • Mitochondria
    • Nucleoid

    ASJC Scopus subject areas

    • Molecular Biology
    • Genetics

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