Different amounts of DNA in each mitochondrion in rice root

Hideki Takanashi, Shin Ichi Arimura, Wataru Sakamoto, Nobuhiro Tsutsumi

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The DNA content of individual mitochondria in rice root cells was analyzed by fluorescence microscopy. Differences in DNA content were detected between individual mitochondria. Some mitochondria contained no detectable nucleoid (DNA-protein complexes). The percent of mitochondria with DAPI(4′,6- Diamidino-2-phenylindole) -stained nucleoids varied over the length of the root (root base, 33%; middle portion of root, 41%; root tip, 91%). The mean amounts of DNA per mitochondrial nucleoid were equivalent to 46.4 kbp in the root base, 52.0 kbp in the middle portion of root and 124.2 kbp in the root tip. The amount of DNA in individual mitochondria and the ratio of mitochondria with visible nucleoids were higher in the root tip than in other parts of the root. The estimated amount of DNA in almost all of the observed mitochondria was smaller than the amount of DNA equivalent to the rice mitochondrial genome size (490 kbp), even in root tip.

Original languageEnglish
Pages (from-to)215-218
Number of pages4
JournalGenes and Genetic Systems
Volume81
Issue number3
DOIs
Publication statusPublished - 2006

Fingerprint

Mitochondria
mitochondria
Meristem
rice
DNA
root tips
Mitochondrial Size
Genome Size
Mitochondrial Genome
Fluorescence microscopy
Oryza
fluorescence microscopy
Mitochondrial DNA
Fluorescence Microscopy
Genes

Keywords

  • DAPI
  • DNA
  • Fluorescence microscopy
  • Mitochondria
  • Nucleoid

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Genetics
  • Genetics(clinical)

Cite this

Different amounts of DNA in each mitochondrion in rice root. / Takanashi, Hideki; Arimura, Shin Ichi; Sakamoto, Wataru; Tsutsumi, Nobuhiro.

In: Genes and Genetic Systems, Vol. 81, No. 3, 2006, p. 215-218.

Research output: Contribution to journalArticle

Takanashi, Hideki ; Arimura, Shin Ichi ; Sakamoto, Wataru ; Tsutsumi, Nobuhiro. / Different amounts of DNA in each mitochondrion in rice root. In: Genes and Genetic Systems. 2006 ; Vol. 81, No. 3. pp. 215-218.
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