TY - JOUR
T1 - Different actions of noradrenaline and nitric oxide on the output of prostaglandins and progesterone in cultured bovine luteal cells
AU - Skarzynski, Dariusz J.
AU - Okuda, Kiyoshi
N1 - Funding Information:
We thank Dr Seiji Ito of Kansai Medical University, Osaka, Japan for antisera of PGF 2α and PGE 2 , and the National Hormone and Pituitary Program, University of Maryland School of Medicine and the National Institute of Diabetes and Digestive and Kidney Disease (NIDDK) for bovine LH (USDA-bLH-B6). The authors also thank Dr Timothy Marczylo for his assistance in preparing this manuscript and Mr Shinya Kobayashi for his technical assistance. D.J.S. was a postdoctoral fellow supported by the JSPS (No. 96346).
PY - 2000/1
Y1 - 2000/1
N2 - The effects of noradrenaline (NA) and nitric oxide (NO) on prostaglandins (PGs) and progesterone (P4) secretion during the development of the bovine corpus luteum (CL) were investigated. Bovine luteal cells of early and mid-cycle CL were cultured for 20 to 24 h in medium containing 10% calf serum, washed, and treated with NA or nitrergic agents for an additional 16 h in a serum-free medium. NA (10-5 M) stimulated P4 from early and mid- cycle CL by 238% and 154% (P < 0.01), respectively. Moreover, although NA induced a twofold increase in PGE2 secretion (P < 0.01) in both examined periods, the effect of NA on PGF(2α) secretion was approximately 1.5 times higher (P < 0.05) in early than in mid-cycle CL. Two NO synthase inhibitors, L-NAME and L-NOARG (both 10-4 M), stimulated P4 secretion only in mid- luteal cells (P < 0.01), although they did not affect the cells from early CL. Although a NO donor, S-NAP (10-4 M) inhibited P4 secretion from mid- cycle luteal cells (P < 0.05), it strongly stimulated PGE2 in both examined phases (P < 0.001). On the other hand, the output of PGF(2α) was stimulated by S-NAP only in the cells of the mid-cycle CL (P < 0.01). The overall results suggest that adrenergic and nitrergic agents play opposite roles in the regulation of bovine CL functions. Whereas NA may play a supporting role in luteal development, NO may participate in the functional regression of the bovine CL by inhibiting steroidogenesis. (C) 2000 Elsevier Science Inc.
AB - The effects of noradrenaline (NA) and nitric oxide (NO) on prostaglandins (PGs) and progesterone (P4) secretion during the development of the bovine corpus luteum (CL) were investigated. Bovine luteal cells of early and mid-cycle CL were cultured for 20 to 24 h in medium containing 10% calf serum, washed, and treated with NA or nitrergic agents for an additional 16 h in a serum-free medium. NA (10-5 M) stimulated P4 from early and mid- cycle CL by 238% and 154% (P < 0.01), respectively. Moreover, although NA induced a twofold increase in PGE2 secretion (P < 0.01) in both examined periods, the effect of NA on PGF(2α) secretion was approximately 1.5 times higher (P < 0.05) in early than in mid-cycle CL. Two NO synthase inhibitors, L-NAME and L-NOARG (both 10-4 M), stimulated P4 secretion only in mid- luteal cells (P < 0.01), although they did not affect the cells from early CL. Although a NO donor, S-NAP (10-4 M) inhibited P4 secretion from mid- cycle luteal cells (P < 0.05), it strongly stimulated PGE2 in both examined phases (P < 0.001). On the other hand, the output of PGF(2α) was stimulated by S-NAP only in the cells of the mid-cycle CL (P < 0.01). The overall results suggest that adrenergic and nitrergic agents play opposite roles in the regulation of bovine CL functions. Whereas NA may play a supporting role in luteal development, NO may participate in the functional regression of the bovine CL by inhibiting steroidogenesis. (C) 2000 Elsevier Science Inc.
KW - Cattle
KW - Corpus luteum
KW - Nitric oxide
KW - Noradrenaline
KW - Prostaglandins
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U2 - 10.1016/S0090-6980(99)00046-5
DO - 10.1016/S0090-6980(99)00046-5
M3 - Article
C2 - 10680774
AN - SCOPUS:0033967971
VL - 60
SP - 35
EP - 47
JO - Journal of Lipid Mediators
JF - Journal of Lipid Mediators
SN - 1098-8823
IS - 1-3
ER -