Differences in the regulatory and functional effects of the Us3 protein kinase activities of herpes simplex virus 1 and 2

Tomomi Morimoto, Jun Arii, Michiko Tanaka, Tetsutaro Sata, Hiroomi Akashi, Masao Yamada, Yukihiro Nishiyama, Masashi Uema, Yasushi Kawaguchi

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Abstract

Us3 protein kinases encoded by herpes simplex virus 1 (HSV-1) and 2 (HSV-2) are serine/threonine protein kinases and play critical roles in viral replication and pathogenicity in vivo. In the present study, we investigated differences in the biological properties of HSV-1 and HSV-2 Us3 protein kinases and demonstrated that HSV-2 Us3 did not have some of the HSV-1 Us3 kinase functions, including control of nuclear egress of nucleocapsids, localization of UL31 and UL34, and cell surface expression of viral envelope glycoprotein B. In agreement with the observations that HSV-2 Us3 was less important for these functions, the effect of HSV-2 Us3 kinase activity on virulence in mice following intracerebral inoculation was much lower than that of HSV-1 Us3. Furthermore, we showed that alanine substitution in HSV-2 Us3 at a site (aspartic acid at position 147) corresponding to one that can be autophosphorylated in HSV-1 Us3 abolished HSV-2 Us3 kinase activity. Thus, the regulatory and functional effects of Us3 kinase activity are different between HSV-1 and HSV-2.

Original languageEnglish
Pages (from-to)11624-11634
Number of pages11
JournalJournal of Virology
Volume83
Issue number22
DOIs
Publication statusPublished - Nov 2009

Fingerprint

Human herpesvirus 2
Human herpesvirus 1
Human Herpesvirus 2
Human Herpesvirus 1
protein kinases
Protein Kinases
phosphotransferases (kinases)
Phosphotransferases
nucleocapsid
aspartic acid
virus replication
alanine
Virulence
glycoproteins
virulence
pathogenicity
Nucleocapsid
Protein-Serine-Threonine Kinases
mice
Aspartic Acid

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Differences in the regulatory and functional effects of the Us3 protein kinase activities of herpes simplex virus 1 and 2. / Morimoto, Tomomi; Arii, Jun; Tanaka, Michiko; Sata, Tetsutaro; Akashi, Hiroomi; Yamada, Masao; Nishiyama, Yukihiro; Uema, Masashi; Kawaguchi, Yasushi.

In: Journal of Virology, Vol. 83, No. 22, 11.2009, p. 11624-11634.

Research output: Contribution to journalArticle

Morimoto, T, Arii, J, Tanaka, M, Sata, T, Akashi, H, Yamada, M, Nishiyama, Y, Uema, M & Kawaguchi, Y 2009, 'Differences in the regulatory and functional effects of the Us3 protein kinase activities of herpes simplex virus 1 and 2', Journal of Virology, vol. 83, no. 22, pp. 11624-11634. https://doi.org/10.1128/JVI.00993-09
Morimoto, Tomomi ; Arii, Jun ; Tanaka, Michiko ; Sata, Tetsutaro ; Akashi, Hiroomi ; Yamada, Masao ; Nishiyama, Yukihiro ; Uema, Masashi ; Kawaguchi, Yasushi. / Differences in the regulatory and functional effects of the Us3 protein kinase activities of herpes simplex virus 1 and 2. In: Journal of Virology. 2009 ; Vol. 83, No. 22. pp. 11624-11634.
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