Differences between Lactococcus garvieae isolated from the genus Seriola in Japan and those isolated from other animals (trout, terrestrial animals from Europe) with regard to pathogenicity, phage susceptibility and genetic characterization

M. Kawanishi, T. Yoshida, S. Yagashiro, M. Kijima, K. Yagyu, T. Nakai, M. Murakami, Hidetoshi Morita, S. Suzuki

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Abstract

Aims: To clarify the epidemiological relationship between Lactococcus garvieae isolates from the Seriola in Japan and isolates from other animals. Methods and Results: A total of 32 isolates obtained from aquatic (the genus Seriola and trout) and terrestrial animals (cow, pig, cat, dog and horse) was used to evaluate its pathogenicity to yellowtail and mouse, phenotype (KG+ and KG-), its susceptibility to three bacteriophages and the pattern of pulsed field gel electrophoresis (PFGE). Lactococcus garvieae isolated from Seriola showed strong pathogenicity to yellowtail, while isolates from trout showed weak pathogenicity and those obtained from terrestrial animals showed no distinct pathogenicity. Only, the isolates from the genus Seriola in Japan showed susceptibility to the bacteriophages. The results of PFGE pattern indicate that the isolates obtained from the Seriola predict homogeneity, while there is no similarity among the isolates obtained from different animals. Conclusion: This experiment indicates that L. garvieae isolated from Seriola in Japan appears to be very different from the isolates obtained from other animals, and the isolates prevalent among the genus Seriola in Japan might be homogeneous. Significance and Impact of the Study: This study suggests that a particular genetic group that has specially adapted and acquired virulence toward yellowtail were prevalent among the genus Seriola in Japan.

Original languageEnglish
Pages (from-to)496-504
Number of pages9
JournalJournal of Applied Microbiology
Volume101
Issue number2
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

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Keywords

  • Capsule
  • Lactococcus garvieae
  • Pathogenicity
  • Phage
  • Pulsed field gel electrophoresis
  • Seriola
  • Terrestrial
  • Trout

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Biotechnology
  • Microbiology

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