Difference in abscisic acid perception mechanisms between closure induction and opening inhibition of stomata

Ye Yin, Yuji Adachi, Wenxiu Ye, Maki Hayashi, Yoshimasa Nakamura, Toshinori Kinoshita, Izumi Mori, Yoshiyuki Murata

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Abstract

Abscisic acid (ABA) induces stomatal closure and inhibits light-induced stomatal opening. The mechanisms in these two processes are not necessarily the same. It has been postulated that the ABA receptors involved in opening inhibition are different from those involved in closure induction. Here, we provide evidence that four recently identified ABA receptors (PYRABACTIN RESISTANCE1 [PYR1], PYRABACTIN RESISTANCE-LIKE1 [PYL1], PYL2, and PYL4) are not sufficient for opening inhibition in Arabidopsis (Arabidopsis thaliana). ABA-induced stomatal closure was impaired in the pyr1/pyl1/pyl2/pyl4 quadruple ABA receptor mutant. ABA inhibition of the opening of the mutant's stomata remained intact. ABA did not induce either the production of reactive oxygen species and nitric oxide or the alkalization of the cytosol in the quadruple mutant, in accordance with the closure phenotype. Whole cell patch-clamp analysis of inward-rectifying K+ current in guard cells showed a partial inhibition by ABA, indicating that the ABA sensitivity of the mutant was not fully impaired. ABA substantially inhibited blue light-induced phosphorylation of H+-ATPase in guard cells in both the mutant and the wild type. On the other hand, in a knockout mutant of the SNF1-related protein kinase, srk2e, stomatal opening and closure, reactive oxygen species and nitric oxide production, cytosolic alkalization, inward-rectifying K+ current inactivation, and H+-ATPase phosphorylation were not sensitive to ABA.

Original languageEnglish
Pages (from-to)600-610
Number of pages11
JournalPlant Physiology
Volume163
Issue number2
DOIs
Publication statusPublished - Oct 2013

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Abscisic Acid
stomata
abscisic acid
mutants
alkalinization
H-transporting ATP synthase
Proton-Translocating ATPases
guard cells
Arabidopsis
receptors
nitric oxide
reactive oxygen species
Reactive Oxygen Species
phosphorylation
Nitric Oxide
Inhibition (Psychology)
Phosphorylation
Light
knockout mutants
blue light

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

Difference in abscisic acid perception mechanisms between closure induction and opening inhibition of stomata. / Yin, Ye; Adachi, Yuji; Ye, Wenxiu; Hayashi, Maki; Nakamura, Yoshimasa; Kinoshita, Toshinori; Mori, Izumi; Murata, Yoshiyuki.

In: Plant Physiology, Vol. 163, No. 2, 10.2013, p. 600-610.

Research output: Contribution to journalArticle

Yin, Ye ; Adachi, Yuji ; Ye, Wenxiu ; Hayashi, Maki ; Nakamura, Yoshimasa ; Kinoshita, Toshinori ; Mori, Izumi ; Murata, Yoshiyuki. / Difference in abscisic acid perception mechanisms between closure induction and opening inhibition of stomata. In: Plant Physiology. 2013 ; Vol. 163, No. 2. pp. 600-610.
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