Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction

Makiko Kawakami, Nobuhiro Harada, Mio Hiratsuka, Kiyoshi Kawai, Yoshimasa Nakamura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We found that both benzyl isothiocyanate (ITC) and phenyl ITC inhibited respiration in the mitochondria in an electrophilic reaction-dependent manner. ITC-induced mitochondrial swelling and cytochrome c release were prevented by cyclosporin A, indicating that they are mediated through the ITC moiety-dependent reaction to critical thiol groups for the opening of membrane permeability transition-dependent pores.

Original languageEnglish
Pages (from-to)2439-2444
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume69
Issue number12
DOIs
Publication statusPublished - 2005

Fingerprint

Isothiocyanates
Mitochondria
isothiocyanates
Swelling
Mitochondrial Swelling
Proteins
Membranes
Cytochromes c
benzyl isothiocyanate
Sulfhydryl Compounds
Cyclosporine
Permeability
Respiration
cyclosporine
cytochrome c
membrane permeability
thiols
cell respiration
mitochondria
isothiocyanic acid

Keywords

  • Cytochrome c
  • Electrophile
  • Isothiocyanate
  • Membrane permeability transition
  • Mitochondria

ASJC Scopus subject areas

  • Bioengineering
  • Biotechnology
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction. / Kawakami, Makiko; Harada, Nobuhiro; Hiratsuka, Mio; Kawai, Kiyoshi; Nakamura, Yoshimasa.

In: Bioscience, Biotechnology and Biochemistry, Vol. 69, No. 12, 2005, p. 2439-2444.

Research output: Contribution to journalArticle

Kawakami, Makiko ; Harada, Nobuhiro ; Hiratsuka, Mio ; Kawai, Kiyoshi ; Nakamura, Yoshimasa. / Dietary isothiocyanates modify mitochondrial functions through their electrophilic reaction. In: Bioscience, Biotechnology and Biochemistry. 2005 ; Vol. 69, No. 12. pp. 2439-2444.
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