Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells

Noriyuki Miyoshi, Yoshimasa Nakamura, Yasuhiro Ueda, Masako Abe, Yoshio Ozawa, Koji Uchida, Toshihiko Osawa

Research output: Contribution to journalArticle

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Abstract

The effects of the constituents isolated from ginger species including curcumin, 6-gingerol and labdane-type diterpene compounds on cell proliferation and the induction of apoptosis in the cultured human T lymphoma Jurkat cells were studied. Among the tested compounds, Galanals A and B, isolated from the flower buds of a Japanese ginger, myoga (Zingiber mioga Roscoe), showed the most potent cytotoxic effect. Exposure of Jurkat human T-cell leukemia cells to galanals resulted in the induction of apoptotic cell death characterized by DNA fragmentation and caspase-3 activation. The mitochondrial damage pathway was suggested to be involved in galanal-induced apoptosis because the treatment of cells with galanals induced mitochondrial transmembrane potential (ΔΨm) alteration and cytochrome c release. The anti-apoptotic Bcl-2 protein was downregulated by the galanal treatment together with enhancement of the Bax expression. In conclusion, the results from this study provide biological evidence that ginger-specific constituents other than curcuminoids are potential anticancer agents.

Original languageEnglish
Pages (from-to)113-119
Number of pages7
JournalCancer Letters
Volume199
Issue number2
DOIs
Publication statusPublished - Sep 25 2003
Externally publishedYes

Fingerprint

Ginger
Jurkat Cells
Lymphoma
Apoptosis
T-Cell Leukemia
Curcumin
Diterpenes
DNA Fragmentation
Cytochromes c
Caspase 3
Antineoplastic Agents
Membrane Potentials
Cell Death
Down-Regulation
Cell Proliferation
galanal A
galanal B
Proteins

Keywords

  • [6]-gingerol
  • Apoptosis
  • Curcumin
  • Galanal
  • Ginger
  • Jurkat cells

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells. / Miyoshi, Noriyuki; Nakamura, Yoshimasa; Ueda, Yasuhiro; Abe, Masako; Ozawa, Yoshio; Uchida, Koji; Osawa, Toshihiko.

In: Cancer Letters, Vol. 199, No. 2, 25.09.2003, p. 113-119.

Research output: Contribution to journalArticle

Miyoshi, Noriyuki ; Nakamura, Yoshimasa ; Ueda, Yasuhiro ; Abe, Masako ; Ozawa, Yoshio ; Uchida, Koji ; Osawa, Toshihiko. / Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells. In: Cancer Letters. 2003 ; Vol. 199, No. 2. pp. 113-119.
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AU - Ozawa, Yoshio

AU - Uchida, Koji

AU - Osawa, Toshihiko

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