Abstract
The effects of the constituents isolated from ginger species including curcumin, 6-gingerol and labdane-type diterpene compounds on cell proliferation and the induction of apoptosis in the cultured human T lymphoma Jurkat cells were studied. Among the tested compounds, Galanals A and B, isolated from the flower buds of a Japanese ginger, myoga (Zingiber mioga Roscoe), showed the most potent cytotoxic effect. Exposure of Jurkat human T-cell leukemia cells to galanals resulted in the induction of apoptotic cell death characterized by DNA fragmentation and caspase-3 activation. The mitochondrial damage pathway was suggested to be involved in galanal-induced apoptosis because the treatment of cells with galanals induced mitochondrial transmembrane potential (ΔΨm) alteration and cytochrome c release. The anti-apoptotic Bcl-2 protein was downregulated by the galanal treatment together with enhancement of the Bax expression. In conclusion, the results from this study provide biological evidence that ginger-specific constituents other than curcuminoids are potential anticancer agents.
| Original language | English |
|---|---|
| Pages (from-to) | 113-119 |
| Number of pages | 7 |
| Journal | Cancer Letters |
| Volume | 199 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Sep 25 2003 |
| Externally published | Yes |
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Keywords
- [6]-gingerol
- Apoptosis
- Curcumin
- Galanal
- Ginger
- Jurkat cells
ASJC Scopus subject areas
- Cancer Research
- Molecular Biology
- Oncology
Cite this
Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells. / Miyoshi, Noriyuki; Nakamura, Yoshimasa; Ueda, Yasuhiro; Abe, Masako; Ozawa, Yoshio; Uchida, Koji; Osawa, Toshihiko.
In: Cancer Letters, Vol. 199, No. 2, 25.09.2003, p. 113-119.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells
AU - Miyoshi, Noriyuki
AU - Nakamura, Yoshimasa
AU - Ueda, Yasuhiro
AU - Abe, Masako
AU - Ozawa, Yoshio
AU - Uchida, Koji
AU - Osawa, Toshihiko
PY - 2003/9/25
Y1 - 2003/9/25
N2 - The effects of the constituents isolated from ginger species including curcumin, 6-gingerol and labdane-type diterpene compounds on cell proliferation and the induction of apoptosis in the cultured human T lymphoma Jurkat cells were studied. Among the tested compounds, Galanals A and B, isolated from the flower buds of a Japanese ginger, myoga (Zingiber mioga Roscoe), showed the most potent cytotoxic effect. Exposure of Jurkat human T-cell leukemia cells to galanals resulted in the induction of apoptotic cell death characterized by DNA fragmentation and caspase-3 activation. The mitochondrial damage pathway was suggested to be involved in galanal-induced apoptosis because the treatment of cells with galanals induced mitochondrial transmembrane potential (ΔΨm) alteration and cytochrome c release. The anti-apoptotic Bcl-2 protein was downregulated by the galanal treatment together with enhancement of the Bax expression. In conclusion, the results from this study provide biological evidence that ginger-specific constituents other than curcuminoids are potential anticancer agents.
AB - The effects of the constituents isolated from ginger species including curcumin, 6-gingerol and labdane-type diterpene compounds on cell proliferation and the induction of apoptosis in the cultured human T lymphoma Jurkat cells were studied. Among the tested compounds, Galanals A and B, isolated from the flower buds of a Japanese ginger, myoga (Zingiber mioga Roscoe), showed the most potent cytotoxic effect. Exposure of Jurkat human T-cell leukemia cells to galanals resulted in the induction of apoptotic cell death characterized by DNA fragmentation and caspase-3 activation. The mitochondrial damage pathway was suggested to be involved in galanal-induced apoptosis because the treatment of cells with galanals induced mitochondrial transmembrane potential (ΔΨm) alteration and cytochrome c release. The anti-apoptotic Bcl-2 protein was downregulated by the galanal treatment together with enhancement of the Bax expression. In conclusion, the results from this study provide biological evidence that ginger-specific constituents other than curcuminoids are potential anticancer agents.
KW - [6]-gingerol
KW - Apoptosis
KW - Curcumin
KW - Galanal
KW - Ginger
KW - Jurkat cells
UR - http://www.scopus.com/inward/record.url?scp=0142060146&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0142060146&partnerID=8YFLogxK
U2 - 10.1016/S0304-3835(03)00381-1
DO - 10.1016/S0304-3835(03)00381-1
M3 - Article
C2 - 12969783
AN - SCOPUS:0142060146
VL - 199
SP - 113
EP - 119
JO - Cancer Letters
JF - Cancer Letters
SN - 0304-3835
IS - 2
ER -