Dietary apple polyphenols have preventive effects against lengthening contraction-induced muscle injuries

Koichi Nakazato, Eisuke Ochi, Toshiaki Waga

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We examined whether polyphenols from dietary apple have protective effects against exercise-induced muscle strain injury. Sixteen male Wistar rats were randomly assigned into the apple polyphenol (APP; N = 8) and control (CON; N = 8) groups. The APP and the CON groups were fed diets with and without 5% APP, respectively. After a 3-wk feeding period, the gastrocnemii of the animals were subject to lengthening contractions with electrical stimulation and forced ankle dorsiflexion. Isometric torques were measured before and after the lengthening contractions and on days 1, 2, 3, 5, and 7 after the contractions. On day 7, the animals were sacrificed and the gastrocnemii harvested. Thiobarbituric-acid-reactive substances, protein carbonyl, and mRNA of antioxidative proteins in the muscles were quantified. The APP group had significantly lower torque deficits than the CON group on days 3, 5, and 7 after the eccentric contractions. The thiobarbituric-acid-reactive substances and protein carbonyl levels in the case of the APP group were significantly lower than those in the case of the CON group. The APP group had significantly higher glutathione-S-transferase a1 mRNA levels than the CON group. Therefore, we conclude that dietary APPs have protective effects against lengthening contraction-induced muscle injury.

Original languageEnglish
Pages (from-to)364-372
Number of pages9
JournalMolecular Nutrition and Food Research
Volume54
Issue number3
DOIs
Publication statusPublished - Mar 2010
Externally publishedYes

Fingerprint

Thiobarbituric Acid Reactive Substances
torque
Torque
Malus
Polyphenols
Muscle Contraction
thiobarbituric acid-reactive substances
protective effect
polyphenols
apples
muscles
Messenger RNA
Muscle Proteins
Wounds and Injuries
muscle protein
Glutathione Transferase
glutathione transferase
Ankle
Electric Stimulation
Wistar Rats

Keywords

  • Apple
  • Injury
  • Lengthening contraction
  • Polyphenol
  • Skeletal muscle

ASJC Scopus subject areas

  • Food Science
  • Biotechnology

Cite this

Dietary apple polyphenols have preventive effects against lengthening contraction-induced muscle injuries. / Nakazato, Koichi; Ochi, Eisuke; Waga, Toshiaki.

In: Molecular Nutrition and Food Research, Vol. 54, No. 3, 03.2010, p. 364-372.

Research output: Contribution to journalArticle

Nakazato, Koichi ; Ochi, Eisuke ; Waga, Toshiaki. / Dietary apple polyphenols have preventive effects against lengthening contraction-induced muscle injuries. In: Molecular Nutrition and Food Research. 2010 ; Vol. 54, No. 3. pp. 364-372.
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