Dichlorophenolindophenol-linked formate dehydrogenase of the methanol-utilizing Mycobacterium gastri MB19

Yoshikazu Izumi, Hiroshi Kanzaki, Shigeru Morita, Nobuo Kato, Hideaki Yamada

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

2,6-Dichlorophenolindophenol (DCIP)-linked formate dehydrogenase activity has been demonstrated for the first time in the cell-free extract of a methylotrophic mycobacterium, Mycobacterium gastri MB19. The enzyme was produced when the strain was cultivated with methanol, glucose or mannitol as a carbon source, whereas no enzyme production occurred with other multi-carbon compounds. The enzyme was located in the particulate fraction. Although the enzyme was unstable on preservation at 4° C in potassium phosphate buffer (pH 7.0), it was stabilized under acidic conditions (pH 5.0). Glycerol and EDTA were also effective for the enzyme's stability. The optimum pH and temperature for the enzyme's activity were 7.0° and 55° C, respectively.

Original languageEnglish
Pages (from-to)277-280
Number of pages4
JournalFEMS Microbiology Letters
Volume56
Issue number3
Publication statusPublished - Dec 15 1988
Externally publishedYes

Fingerprint

Formate Dehydrogenases
2,6-Dichloroindophenol
Mycobacterium
Methanol
Enzymes
Carbon
Enzyme Stability
Mannitol
Cell Extracts
Edetic Acid
Glycerol
Buffers
Glucose
Temperature

Keywords

  • Formate dehydrogenase
  • Methylotroph
  • Mycobacterium gastri

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Izumi, Y., Kanzaki, H., Morita, S., Kato, N., & Yamada, H. (1988). Dichlorophenolindophenol-linked formate dehydrogenase of the methanol-utilizing Mycobacterium gastri MB19. FEMS Microbiology Letters, 56(3), 277-280.

Dichlorophenolindophenol-linked formate dehydrogenase of the methanol-utilizing Mycobacterium gastri MB19. / Izumi, Yoshikazu; Kanzaki, Hiroshi; Morita, Shigeru; Kato, Nobuo; Yamada, Hideaki.

In: FEMS Microbiology Letters, Vol. 56, No. 3, 15.12.1988, p. 277-280.

Research output: Contribution to journalArticle

Izumi, Y, Kanzaki, H, Morita, S, Kato, N & Yamada, H 1988, 'Dichlorophenolindophenol-linked formate dehydrogenase of the methanol-utilizing Mycobacterium gastri MB19', FEMS Microbiology Letters, vol. 56, no. 3, pp. 277-280.
Izumi, Yoshikazu ; Kanzaki, Hiroshi ; Morita, Shigeru ; Kato, Nobuo ; Yamada, Hideaki. / Dichlorophenolindophenol-linked formate dehydrogenase of the methanol-utilizing Mycobacterium gastri MB19. In: FEMS Microbiology Letters. 1988 ; Vol. 56, No. 3. pp. 277-280.
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