Degradation of dichloromethane (DCM) by two environmental isolates, Flevimones sp. strain P3310 and Chryseobacterum sp. strain G31, were studied. The ability of the strains was raised to degrade 3,000 mg/l of DCM by acclimatization, although the original isolates could degrade less than 500 mg/l. The first step in the degradation process was dechlorination, and the liberated chloride ions caused the reduction of pH and the bacterial growth; the addition of phosphate salts, however, restored the growth and the degrading ability of the culture by increasing the buffer capacity. The DCM-degrading activity was also detected in the cell-free extract and the culture-supernatant. These results suggest that the isolates or their products are possible candidates for bioremediation to eliminate DCM pollution.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology
- Public Health, Environmental and Occupational Health