Diabetic cystopathy correlates with a long-term decrease in nerve growth factor levels in the bladder and lumbosacral dorsal root ganglia

Katsumi Sasaki, Michael B. Chancellor, Michael W. Phelan, Teruhiko Yokoyama, Matthew O. Fraser, Satoshi Seki, Kazuyuki Kubo, Hiromi Kumon, William C.de Groat, Naoki Yoshimura

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Abstract

Purpose: It has been proposed that a deficiency in the axonal transport of nerve growth factor (NGF) may have an important role in inducing diabetic neuropathy, which contributes to diabetic cystopathy. Therefore, in streptozotocin (Sigma Chemical Co., St. Louis, Missouri) induced diabetic rats we investigated the relationship of bladder function with NGF levels in the bladder and lumbosacral dorsal root ganglia, which contain afferent neurons innervating the bladder. Materials and Methods: At 6 and 12 weeks after the induction of diabetes with streptozotocin (65 mg./kg. intraperitoneally) the effects of diabetes on Aδ afferent fiber dependent, conscious voiding were evaluated by metabolic cage measurements and awake cystometry. The effects of diabetes on C-fiber mediated bladder nociceptive responses were also investigated by cystometry with intravesical instillation of 0.25% acetic acid in the rats under urethane anesthesia. NGF levels in the bladder and L6 to S1 dorsal root ganglia were measured by enzyme-linked immunosorbent assay 3, 6, 9 and 12 weeks after streptozotocin injection. Results: In diabetic rats NGF levels in the bladder and L6 to S1 dorsal root ganglia were significantly decreased 12 weeks after streptozotocin injection (p <0.01). In cystometry and metabolic cage studies bladder capacity and post-void residual volume were significantly increased 12 weeks after streptozotocin injection (p <0.01). Bladder nociceptive responses revealed by a reduction in inter-contraction intervals after acetic acid infusion were significantly decreased in a time dependent manner 12 weeks after streptozotocin injection. Conclusions: Rats with streptozotocin induced diabetes mellitus showed a significant time dependent decrease in NGF levels in the bladder and L6 to S1 dorsal root ganglia that was associated with voiding dysfunction attributable to defects in Aδ and C-fiber bladder afferents. Therefore, reduced production of NGF in the bladder and/or impaired transport of NGF to L6 to S1 dorsal root ganglia, which contain bladder afferent neurons, may be an important mechanism inducing diabetic cystopathy.

Original languageEnglish
Pages (from-to)1259-1264
Number of pages6
JournalJournal of Urology
Volume168
Issue number3
DOIs
Publication statusPublished - Sep 2002

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Keywords

  • Bladder, neurogenic
  • Diabetes mellitus
  • Ganglia, spinal
  • Nerve growth factor
  • Rats, Sprague-Dawley

ASJC Scopus subject areas

  • Urology

Cite this

Sasaki, K., Chancellor, M. B., Phelan, M. W., Yokoyama, T., Fraser, M. O., Seki, S., Kubo, K., Kumon, H., Groat, W. C. D., & Yoshimura, N. (2002). Diabetic cystopathy correlates with a long-term decrease in nerve growth factor levels in the bladder and lumbosacral dorsal root ganglia. Journal of Urology, 168(3), 1259-1264. https://doi.org/10.1016/S0022-5347(05)64636-8