Diabetes-induced oxidative stress is mediated by Ca2+- independent phospholipase A2 in neutrophils

Srinivas Ayilavarapu, Alpdogan Kantarci, Gabrielle Fredman, Oya Turkoglu, Kazuhiro Omori, Hongsheng Liu, Tomoyuki Iwata, Motohiko Yagi, Hatice Hasturk, Thomas E. Van Dyke

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Abstract

Neutrophils from people with poorly controlled diabetes present a primed phenotype and secrete excessive superoxide. Phospholipase A2 (PLA2)-derived arachidonic acid (AA) activates the assembly of NADPH oxidase to generate superoxide anion. There is a gap in the current literature regarding which PLA2 isoform regulates NADPH oxidase activation. The aim of this study was to identify the PLA2 isoform involved in the regulation of superoxide generation in neutrophils and investigate if PLA 2 mediates priming in response to pathologic hyperglycemia. Neutrophils were isolated from people with diabetes mellitus and healthy controls, and HL60 neutrophil-like cells were grown in hyperglycemic conditions. Incubating neutrophils with the Ca2+-independent PLA2 (iPLA2) inhibitor bromoenol lactone (BEL) completely suppressed fMLP-induced generation of superoxide. The nonspecific actions of BEL on phosphatidic acid phosphohydrolase-1, p47phox phosphorylation, and apoptosis were ruled out by specific assays. Small interfering RNA knockdown of iPLA2 inhibited superoxide generation by neutrophils. Neutrophils from people with poorly controlled diabetes and in vitro incubation of neutrophils with high glucose and the receptor for advanced glycation end products ligand S100B greatly enhanced superoxide generation compared with controls, and this was signifi-cantly inhibited by BEL. A modified iPLA 2 assay, Western blotting, and PCR confirmed that there was increased iPLA2 activity and expression in neutrophils from people with diabetes. AA (10 μM) partly rescued the inhibition of superoxide generation mediated by BEL, confirming that NADPH oxidase activity is, in part, regulated by AA. This study provides evidence for the role of iPLA2 in enhanced superoxide generation in neutrophils from people with diabetes mellitus and presents an alternate pathway independent of protein kinase C and phosphatidic acid phosphohydrolase-1 hydrolase signaling.

Original languageEnglish
Pages (from-to)1507-1515
Number of pages9
JournalJournal of Immunology
Volume184
Issue number3
DOIs
Publication statusPublished - Feb 1 2010

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Phospholipases A2
Oxidative Stress
Neutrophils
Superoxides
NADPH Oxidase
Phosphatidate Phosphatase
Arachidonic Acid
Diabetes Mellitus
Protein Isoforms
Calcium-Independent Phospholipase A2
Hydrolases
Hyperglycemia
Protein Kinase C
Small Interfering RNA
Western Blotting
Phosphorylation
Apoptosis
Ligands
Phenotype
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology
  • Medicine(all)

Cite this

Ayilavarapu, S., Kantarci, A., Fredman, G., Turkoglu, O., Omori, K., Liu, H., ... Van Dyke, T. E. (2010). Diabetes-induced oxidative stress is mediated by Ca2+- independent phospholipase A2 in neutrophils. Journal of Immunology, 184(3), 1507-1515. https://doi.org/10.4049/jimmunol.0901219

Diabetes-induced oxidative stress is mediated by Ca2+- independent phospholipase A2 in neutrophils. / Ayilavarapu, Srinivas; Kantarci, Alpdogan; Fredman, Gabrielle; Turkoglu, Oya; Omori, Kazuhiro; Liu, Hongsheng; Iwata, Tomoyuki; Yagi, Motohiko; Hasturk, Hatice; Van Dyke, Thomas E.

In: Journal of Immunology, Vol. 184, No. 3, 01.02.2010, p. 1507-1515.

Research output: Contribution to journalArticle

Ayilavarapu, S, Kantarci, A, Fredman, G, Turkoglu, O, Omori, K, Liu, H, Iwata, T, Yagi, M, Hasturk, H & Van Dyke, TE 2010, 'Diabetes-induced oxidative stress is mediated by Ca2+- independent phospholipase A2 in neutrophils', Journal of Immunology, vol. 184, no. 3, pp. 1507-1515. https://doi.org/10.4049/jimmunol.0901219
Ayilavarapu, Srinivas ; Kantarci, Alpdogan ; Fredman, Gabrielle ; Turkoglu, Oya ; Omori, Kazuhiro ; Liu, Hongsheng ; Iwata, Tomoyuki ; Yagi, Motohiko ; Hasturk, Hatice ; Van Dyke, Thomas E. / Diabetes-induced oxidative stress is mediated by Ca2+- independent phospholipase A2 in neutrophils. In: Journal of Immunology. 2010 ; Vol. 184, No. 3. pp. 1507-1515.
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