Developmental delay in islet-1-positive motor neurons in chick spina bifida

Min Wang, Katsumi Mominoki, Masae Kinutani, Zhong Wang, Naoto Kobayashi, Tetsuya Shimokawa, Hiroaki Nabeka, Takashi Fujiwara, Seiji Matsuda

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Spina bifida aperta (SBA) is a congenital malformation of the spinal cord with complications such as spinal ataxia and bowel and bladder dysfunction. We have developed a chick model with surgery-induced SBA that shows spinal ataxia after hatching. In the present study, motor neurons in the early stages in chicks with and without SBA were observed by immunohistochemical staining with a monoclonal antibody against Islet-1, a motor neuron marker. Delay in migration and maturation of motor neurons was observed in SBA. Although the final numbers of Islet-1-positive neurons in these two groups were not different, a defect in the production and elimination of excess motor neurons in the early developmental stages in the SBA group may be involved in the pathological mechanism of the motor complications of this disease.

Original languageEnglish
Pages (from-to)447-452
Number of pages6
JournalJournal of Veterinary Medical Science
Volume73
Issue number4
DOIs
Publication statusPublished - Apr 2011

Fingerprint

Spina Bifida Cystica
spina bifida
Spinal Dysraphism
Motor Neurons
motor neurons
chicks
Ataxia
congenital abnormalities
complications (disease)
bladder
spinal cord
monoclonal antibodies
Spinal Cord
Urinary Bladder
hatching
surgery
neurons
Monoclonal Antibodies
developmental stages
Staining and Labeling

Keywords

  • Animal model for human disease
  • Chick
  • Motor neurons
  • Neural cell differentiation
  • Spina bifida aperta

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Wang, M., Mominoki, K., Kinutani, M., Wang, Z., Kobayashi, N., Shimokawa, T., ... Matsuda, S. (2011). Developmental delay in islet-1-positive motor neurons in chick spina bifida. Journal of Veterinary Medical Science, 73(4), 447-452. https://doi.org/10.1292/jvms.10-0385

Developmental delay in islet-1-positive motor neurons in chick spina bifida. / Wang, Min; Mominoki, Katsumi; Kinutani, Masae; Wang, Zhong; Kobayashi, Naoto; Shimokawa, Tetsuya; Nabeka, Hiroaki; Fujiwara, Takashi; Matsuda, Seiji.

In: Journal of Veterinary Medical Science, Vol. 73, No. 4, 04.2011, p. 447-452.

Research output: Contribution to journalArticle

Wang, M, Mominoki, K, Kinutani, M, Wang, Z, Kobayashi, N, Shimokawa, T, Nabeka, H, Fujiwara, T & Matsuda, S 2011, 'Developmental delay in islet-1-positive motor neurons in chick spina bifida', Journal of Veterinary Medical Science, vol. 73, no. 4, pp. 447-452. https://doi.org/10.1292/jvms.10-0385
Wang, Min ; Mominoki, Katsumi ; Kinutani, Masae ; Wang, Zhong ; Kobayashi, Naoto ; Shimokawa, Tetsuya ; Nabeka, Hiroaki ; Fujiwara, Takashi ; Matsuda, Seiji. / Developmental delay in islet-1-positive motor neurons in chick spina bifida. In: Journal of Veterinary Medical Science. 2011 ; Vol. 73, No. 4. pp. 447-452.
@article{b0420bf40d2b4579ab17f68d1083cb61,
title = "Developmental delay in islet-1-positive motor neurons in chick spina bifida",
abstract = "Spina bifida aperta (SBA) is a congenital malformation of the spinal cord with complications such as spinal ataxia and bowel and bladder dysfunction. We have developed a chick model with surgery-induced SBA that shows spinal ataxia after hatching. In the present study, motor neurons in the early stages in chicks with and without SBA were observed by immunohistochemical staining with a monoclonal antibody against Islet-1, a motor neuron marker. Delay in migration and maturation of motor neurons was observed in SBA. Although the final numbers of Islet-1-positive neurons in these two groups were not different, a defect in the production and elimination of excess motor neurons in the early developmental stages in the SBA group may be involved in the pathological mechanism of the motor complications of this disease.",
keywords = "Animal model for human disease, Chick, Motor neurons, Neural cell differentiation, Spina bifida aperta",
author = "Min Wang and Katsumi Mominoki and Masae Kinutani and Zhong Wang and Naoto Kobayashi and Tetsuya Shimokawa and Hiroaki Nabeka and Takashi Fujiwara and Seiji Matsuda",
year = "2011",
month = "4",
doi = "10.1292/jvms.10-0385",
language = "English",
volume = "73",
pages = "447--452",
journal = "Journal of Veterinary Medical Science",
issn = "0916-7250",
publisher = "Japanese Society of Veterinary Science",
number = "4",

}

TY - JOUR

T1 - Developmental delay in islet-1-positive motor neurons in chick spina bifida

AU - Wang, Min

AU - Mominoki, Katsumi

AU - Kinutani, Masae

AU - Wang, Zhong

AU - Kobayashi, Naoto

AU - Shimokawa, Tetsuya

AU - Nabeka, Hiroaki

AU - Fujiwara, Takashi

AU - Matsuda, Seiji

PY - 2011/4

Y1 - 2011/4

N2 - Spina bifida aperta (SBA) is a congenital malformation of the spinal cord with complications such as spinal ataxia and bowel and bladder dysfunction. We have developed a chick model with surgery-induced SBA that shows spinal ataxia after hatching. In the present study, motor neurons in the early stages in chicks with and without SBA were observed by immunohistochemical staining with a monoclonal antibody against Islet-1, a motor neuron marker. Delay in migration and maturation of motor neurons was observed in SBA. Although the final numbers of Islet-1-positive neurons in these two groups were not different, a defect in the production and elimination of excess motor neurons in the early developmental stages in the SBA group may be involved in the pathological mechanism of the motor complications of this disease.

AB - Spina bifida aperta (SBA) is a congenital malformation of the spinal cord with complications such as spinal ataxia and bowel and bladder dysfunction. We have developed a chick model with surgery-induced SBA that shows spinal ataxia after hatching. In the present study, motor neurons in the early stages in chicks with and without SBA were observed by immunohistochemical staining with a monoclonal antibody against Islet-1, a motor neuron marker. Delay in migration and maturation of motor neurons was observed in SBA. Although the final numbers of Islet-1-positive neurons in these two groups were not different, a defect in the production and elimination of excess motor neurons in the early developmental stages in the SBA group may be involved in the pathological mechanism of the motor complications of this disease.

KW - Animal model for human disease

KW - Chick

KW - Motor neurons

KW - Neural cell differentiation

KW - Spina bifida aperta

UR - http://www.scopus.com/inward/record.url?scp=79955774006&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955774006&partnerID=8YFLogxK

U2 - 10.1292/jvms.10-0385

DO - 10.1292/jvms.10-0385

M3 - Article

C2 - 21127393

AN - SCOPUS:79955774006

VL - 73

SP - 447

EP - 452

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

SN - 0916-7250

IS - 4

ER -