Developmental change of kidney dopamine receptors in spontaneously hypertensive rats

H. Watanabe, T. Ogura, M. Hosoya, J. Kageyama, Z. Ota

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To elucidate the role of the kidney dopamine system on blood pressure regulation, we investigated the developmental change of kidney dopamine receptors in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. The autoradiogram of [3H]-SCH23390, a specific DA1 receptor antagonist, showed that DA1 receptors were localized mainly in the rat renal cortex. The radiolabeled receptor assay (RRA) of [3H]-spiperone was performed on 3-, 7-, and 18-week-old SHR using the homogenate of renal cortex. Neither dissociation constant (K(d)) nor maximum binding capacity (B(max)) were different between SHR and WKY rats at the age of 3 and 7 weeks. Nevertheless, K'Φ and B(max), were significantly low in 18-week-old SHR. The systolic blood pressure of 7-, and 18-week-old SHR was significantly higher than that of age matched WKY rats. The urinary dopamine excretion in 16- week-old SHR was significantly higher than that in age-matched WKY rats, although urine volume and urinary sodium excretion were the same as those in the control in both sodium-loaded and -restricted state. In summary, although renal dopamine production was enhanced in SHR in the hypertensive state, dopamine was found not to contribute to natriuresis since the number of dopamine receptors was reduced in these rats. We also found that enhanced dopamine production in response to a salt load was lacking in these rats. These two pathological phenomena noted in the renal dopamine system play a role in the progression of hypertension in SHR.

Original languageEnglish
Pages (from-to)333-344
Number of pages12
JournalResearch Communications in Molecular Pathology and Pharmacology
Volume87
Issue number3
Publication statusPublished - 1995

Fingerprint

Dopamine Receptors
Inbred SHR Rats
Kidney
Dopamine
Inbred WKY Rats
Blood Pressure
Sodium
Spiperone
Natriuresis
Salts
Urine
Hypertension

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Developmental change of kidney dopamine receptors in spontaneously hypertensive rats. / Watanabe, H.; Ogura, T.; Hosoya, M.; Kageyama, J.; Ota, Z.

In: Research Communications in Molecular Pathology and Pharmacology, Vol. 87, No. 3, 1995, p. 333-344.

Research output: Contribution to journalArticle

Watanabe, H. ; Ogura, T. ; Hosoya, M. ; Kageyama, J. ; Ota, Z. / Developmental change of kidney dopamine receptors in spontaneously hypertensive rats. In: Research Communications in Molecular Pathology and Pharmacology. 1995 ; Vol. 87, No. 3. pp. 333-344.
@article{a825b6fe577a45e985bc8ac78ba35904,
title = "Developmental change of kidney dopamine receptors in spontaneously hypertensive rats",
abstract = "To elucidate the role of the kidney dopamine system on blood pressure regulation, we investigated the developmental change of kidney dopamine receptors in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. The autoradiogram of [3H]-SCH23390, a specific DA1 receptor antagonist, showed that DA1 receptors were localized mainly in the rat renal cortex. The radiolabeled receptor assay (RRA) of [3H]-spiperone was performed on 3-, 7-, and 18-week-old SHR using the homogenate of renal cortex. Neither dissociation constant (K(d)) nor maximum binding capacity (B(max)) were different between SHR and WKY rats at the age of 3 and 7 weeks. Nevertheless, K'Φ and B(max), were significantly low in 18-week-old SHR. The systolic blood pressure of 7-, and 18-week-old SHR was significantly higher than that of age matched WKY rats. The urinary dopamine excretion in 16- week-old SHR was significantly higher than that in age-matched WKY rats, although urine volume and urinary sodium excretion were the same as those in the control in both sodium-loaded and -restricted state. In summary, although renal dopamine production was enhanced in SHR in the hypertensive state, dopamine was found not to contribute to natriuresis since the number of dopamine receptors was reduced in these rats. We also found that enhanced dopamine production in response to a salt load was lacking in these rats. These two pathological phenomena noted in the renal dopamine system play a role in the progression of hypertension in SHR.",
author = "H. Watanabe and T. Ogura and M. Hosoya and J. Kageyama and Z. Ota",
year = "1995",
language = "English",
volume = "87",
pages = "333--344",
journal = "Research Communications in Molecular Pathology and Pharmacology",
issn = "1078-0297",
publisher = "PJD Publications Ltd",
number = "3",

}

TY - JOUR

T1 - Developmental change of kidney dopamine receptors in spontaneously hypertensive rats

AU - Watanabe, H.

AU - Ogura, T.

AU - Hosoya, M.

AU - Kageyama, J.

AU - Ota, Z.

PY - 1995

Y1 - 1995

N2 - To elucidate the role of the kidney dopamine system on blood pressure regulation, we investigated the developmental change of kidney dopamine receptors in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. The autoradiogram of [3H]-SCH23390, a specific DA1 receptor antagonist, showed that DA1 receptors were localized mainly in the rat renal cortex. The radiolabeled receptor assay (RRA) of [3H]-spiperone was performed on 3-, 7-, and 18-week-old SHR using the homogenate of renal cortex. Neither dissociation constant (K(d)) nor maximum binding capacity (B(max)) were different between SHR and WKY rats at the age of 3 and 7 weeks. Nevertheless, K'Φ and B(max), were significantly low in 18-week-old SHR. The systolic blood pressure of 7-, and 18-week-old SHR was significantly higher than that of age matched WKY rats. The urinary dopamine excretion in 16- week-old SHR was significantly higher than that in age-matched WKY rats, although urine volume and urinary sodium excretion were the same as those in the control in both sodium-loaded and -restricted state. In summary, although renal dopamine production was enhanced in SHR in the hypertensive state, dopamine was found not to contribute to natriuresis since the number of dopamine receptors was reduced in these rats. We also found that enhanced dopamine production in response to a salt load was lacking in these rats. These two pathological phenomena noted in the renal dopamine system play a role in the progression of hypertension in SHR.

AB - To elucidate the role of the kidney dopamine system on blood pressure regulation, we investigated the developmental change of kidney dopamine receptors in spontaneously hypertensive rats (SHR) and Wistar-Kyoto (WKY) rats. The autoradiogram of [3H]-SCH23390, a specific DA1 receptor antagonist, showed that DA1 receptors were localized mainly in the rat renal cortex. The radiolabeled receptor assay (RRA) of [3H]-spiperone was performed on 3-, 7-, and 18-week-old SHR using the homogenate of renal cortex. Neither dissociation constant (K(d)) nor maximum binding capacity (B(max)) were different between SHR and WKY rats at the age of 3 and 7 weeks. Nevertheless, K'Φ and B(max), were significantly low in 18-week-old SHR. The systolic blood pressure of 7-, and 18-week-old SHR was significantly higher than that of age matched WKY rats. The urinary dopamine excretion in 16- week-old SHR was significantly higher than that in age-matched WKY rats, although urine volume and urinary sodium excretion were the same as those in the control in both sodium-loaded and -restricted state. In summary, although renal dopamine production was enhanced in SHR in the hypertensive state, dopamine was found not to contribute to natriuresis since the number of dopamine receptors was reduced in these rats. We also found that enhanced dopamine production in response to a salt load was lacking in these rats. These two pathological phenomena noted in the renal dopamine system play a role in the progression of hypertension in SHR.

UR - http://www.scopus.com/inward/record.url?scp=0028952848&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028952848&partnerID=8YFLogxK

M3 - Article

C2 - 7620826

AN - SCOPUS:0028952848

VL - 87

SP - 333

EP - 344

JO - Research Communications in Molecular Pathology and Pharmacology

JF - Research Communications in Molecular Pathology and Pharmacology

SN - 1078-0297

IS - 3

ER -