Abstract
Bovine follicular oocytes from individual heifers (n=49) were separately matured, fertilized with frozen-thawed spermatozoa and cultured with cumulus cells. Although there were great variations in the number (mean±SD=19.1±11.9) of oocytes collected from individual heifers and the percentages of the oocytes cleaved 48 hours after insemination (mean±SD=69.5±18.4) and developed to the morula stage 7 days after insemination (mean±SD=10.9±10.9), there were significant correlations between the numbers of oocytes collected and cleaved (the correlation coefficient: r= 0.9336) or developed to morula stage (r=0.6633), indicating that oocytes from different heifers have the same developmental ability after in vitro fertilization. Ten morulae and 12 blastocysts which were obtained 7 and 8 days after insemination were transferred, one by one, to each uterine horn of 11 recipients. At Day 60 of pregnancy, 8 (80%) fetuses were identified in 4 (80%) of 5 recipients into which 10 embryos were transferred at Day -1 of synchrony. However, only 3 (25%) fetuses were identified in 2 (40%) of 6 recipients into which 12 embryos were transferred at Day 0 or +1 of synchrony.
| Original language | English |
|---|---|
| Pages (from-to) | 427-434 |
| Number of pages | 8 |
| Journal | Theriogenology |
| Volume | 36 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Sept 1991 |
| Externally published | Yes |
Keywords
- bovine
- co-culture
- embryo
- in vitro fertilization
- oocyte
ASJC Scopus subject areas
- Small Animals
- Food Animals
- Animal Science and Zoology
- Equine