Developmental capacity of bovine oocytes collected from ovaries of individual heifers and fertilized in vitro

H. Funahashi, Y. Aoyagi, T. Takeda, T. Onihara

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Bovine follicular oocytes from individual heifers (n=49) were separately matured, fertilized with frozen-thawed spermatozoa and cultured with cumulus cells. Although there were great variations in the number (mean±SD=19.1±11.9) of oocytes collected from individual heifers and the percentages of the oocytes cleaved 48 hours after insemination (mean±SD=69.5±18.4) and developed to the morula stage 7 days after insemination (mean±SD=10.9±10.9), there were significant correlations between the numbers of oocytes collected and cleaved (the correlation coefficient: r= 0.9336) or developed to morula stage (r=0.6633), indicating that oocytes from different heifers have the same developmental ability after in vitro fertilization. Ten morulae and 12 blastocysts which were obtained 7 and 8 days after insemination were transferred, one by one, to each uterine horn of 11 recipients. At Day 60 of pregnancy, 8 (80%) fetuses were identified in 4 (80%) of 5 recipients into which 10 embryos were transferred at Day -1 of synchrony. However, only 3 (25%) fetuses were identified in 2 (40%) of 6 recipients into which 12 embryos were transferred at Day 0 or +1 of synchrony.

Original languageEnglish
Pages (from-to)427-434
Number of pages8
JournalTheriogenology
Volume36
Issue number3
DOIs
Publication statusPublished - Sep 1991
Externally publishedYes

Keywords

  • bovine
  • co-culture
  • embryo
  • in vitro fertilization
  • oocyte

ASJC Scopus subject areas

  • Small Animals
  • Food Animals
  • Animal Science and Zoology
  • Equine

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