Developmental capacity of bovine oocytes collected from ovaries of individual heifers and fertilized in vitro

Hiroaki Funahashi, Y. Aoyagi, T. Takeda, T. Onihara

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Bovine follicular oocytes from individual heifers (n=49) were separately matured, fertilized with frozen-thawed spermatozoa and cultured with cumulus cells. Although there were great variations in the number (mean±SD=19.1±11.9) of oocytes collected from individual heifers and the percentages of the oocytes cleaved 48 hours after insemination (mean±SD=69.5±18.4) and developed to the morula stage 7 days after insemination (mean±SD=10.9±10.9), there were significant correlations between the numbers of oocytes collected and cleaved (the correlation coefficient: r= 0.9336) or developed to morula stage (r=0.6633), indicating that oocytes from different heifers have the same developmental ability after in vitro fertilization. Ten morulae and 12 blastocysts which were obtained 7 and 8 days after insemination were transferred, one by one, to each uterine horn of 11 recipients. At Day 60 of pregnancy, 8 (80%) fetuses were identified in 4 (80%) of 5 recipients into which 10 embryos were transferred at Day -1 of synchrony. However, only 3 (25%) fetuses were identified in 2 (40%) of 6 recipients into which 12 embryos were transferred at Day 0 or +1 of synchrony.

Original languageEnglish
Pages (from-to)427-434
Number of pages8
JournalTheriogenology
Volume36
Issue number3
DOIs
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Oocytes
heifers
Ovary
oocytes
Morula
Insemination
morula
insemination
cattle
fetus
embryo (animal)
Fetus
Embryonic Structures
Cumulus Cells
in vitro fertilization
Blastocyst
Fertilization in Vitro
blastocyst
Spermatozoa
spermatozoa

Keywords

  • bovine
  • co-culture
  • embryo
  • in vitro fertilization
  • oocyte

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Developmental capacity of bovine oocytes collected from ovaries of individual heifers and fertilized in vitro. / Funahashi, Hiroaki; Aoyagi, Y.; Takeda, T.; Onihara, T.

In: Theriogenology, Vol. 36, No. 3, 1991, p. 427-434.

Research output: Contribution to journalArticle

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