Developmental and ultrastructual characteristics of mouse oocytes grown in vitro from primordial germ cells.

Hideyuki H. Motohashi, Hidemi Kada, Kahei Sato

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of this study was to clarify the developmental and ultrastructual characteristics of oocytes grown in vitro from primordial germ cells. The female genital ridges at 12.5 days post coitus were cultured for 18 days on an insert membrane in Waymouth's MB752/1 medium, supplemented with 15% fetal bovine serum and 1 mM sodium pyruvate; subsequently, the follicles isolated from the tissue were cultured for eight days in Waymouth's medium supplemented with 5 microg/ml insulin, 5 microg/ml transferrin, 5 ng/ml selenium, 10 mIU/ml follicle stimulating hormone, and 100 ng/ml stem cell factor. The primordial germ cells developed in vitro into oocytes of more than 60 microm in diameter. The transmission electron microscopic analysis indicated that the oocytes, which developed in vitro, showed no obvious abnormality in their ultrastructure and had organelles appropriate for the oocyte size. However, a delay in the progressive changes of morphology in some of the organelles during oocyte growth was often found when comparing them to oocytes grown in vivo.

Original languageEnglish
Pages (from-to)67-74
Number of pages8
JournalHuman cell : official journal of Human Cell Research Society
Volume17
Issue number1
Publication statusPublished - Mar 2004
Externally publishedYes

Fingerprint

Germ Cells
Oocytes
Organelles
Stem Cell Factor
Coitus
Follicle Stimulating Hormone
Transferrin
Selenium
Pyruvic Acid
In Vitro Techniques
Sodium
Electrons
Insulin
Membranes
Growth
Serum

ASJC Scopus subject areas

  • Cell Biology
  • Cancer Research

Cite this

Developmental and ultrastructual characteristics of mouse oocytes grown in vitro from primordial germ cells. / Motohashi, Hideyuki H.; Kada, Hidemi; Sato, Kahei.

In: Human cell : official journal of Human Cell Research Society, Vol. 17, No. 1, 03.2004, p. 67-74.

Research output: Contribution to journalArticle

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