Developmental and tissue-specific expression of CHS1 from Plutella xylostella and its response to chlorfluazuron

Muhammad Ashfaq; Shoji Sonoda; Hisaaki Tsumuki

doi:10.1016/j.pestbp.2007.02.004

Developmental and tissue-specific expression of CHS1 from Plutella xylostella and its response to chlorfluazuron

Muhammad Ashfaq, Shoji Sonoda, Hisaaki Tsumuki

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

The cDNA of chitin synthase-1 gene of Plutella xylostella (PxCHS1) was characterized and expression patterns of the two splice variants PxCHS1A and PxCHS1B were investigated in various developmental stages and in major body tissues by RT-PCR and real-time quantitative PCR (qPCR). The PxCHS1 cDNA was 5461 bp in length with an open reading frame of 4701 bp that encoded a putative protein of 1567 amino acids with predicted molecular mass of 179 kDa. The two splice variants were expressed from the mutually exclusive exons which were same in size (177 bp) but showed only 66% identity at the nucleotide level. Both splice variants were expressed in all developmental stages. The qPCR data suggested an uneven expression of the two variants in the body where expression of PxCHS1A was 3.7-fold higher than that of PxCHS1B. The expression of PxCHS1A was 1.5-fold higher in the head than the body whereas in case of PxCHS1B the difference between head and body was 6.3-fold. Chlorfluazuron did not change the expression of PxCHS1 in larvae.

Original language	English
Pages (from-to)	20-30
Number of pages	11
Journal	Pesticide Biochemistry and Physiology
Volume	89
Issue number	1
DOIs	https://doi.org/10.1016/j.pestbp.2007.02.004
Publication status	Published - Sept 2007

Keywords

Chitin synthase
Chlorfluazuron
Gene expression
Plutella xylostella
Splice variants

ASJC Scopus subject areas

Agronomy and Crop Science
Health, Toxicology and Mutagenesis

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10.1016/j.pestbp.2007.02.004

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title = "Developmental and tissue-specific expression of CHS1 from Plutella xylostella and its response to chlorfluazuron",

abstract = "The cDNA of chitin synthase-1 gene of Plutella xylostella (PxCHS1) was characterized and expression patterns of the two splice variants PxCHS1A and PxCHS1B were investigated in various developmental stages and in major body tissues by RT-PCR and real-time quantitative PCR (qPCR). The PxCHS1 cDNA was 5461 bp in length with an open reading frame of 4701 bp that encoded a putative protein of 1567 amino acids with predicted molecular mass of 179 kDa. The two splice variants were expressed from the mutually exclusive exons which were same in size (177 bp) but showed only 66% identity at the nucleotide level. Both splice variants were expressed in all developmental stages. The qPCR data suggested an uneven expression of the two variants in the body where expression of PxCHS1A was 3.7-fold higher than that of PxCHS1B. The expression of PxCHS1A was 1.5-fold higher in the head than the body whereas in case of PxCHS1B the difference between head and body was 6.3-fold. Chlorfluazuron did not change the expression of PxCHS1 in larvae.",

keywords = "Chitin synthase, Chlorfluazuron, Gene expression, Plutella xylostella, Splice variants",

author = "Muhammad Ashfaq and Shoji Sonoda and Hisaaki Tsumuki",

note = "Funding Information: This study was supported in part by a Grant-in-Aid for Encouragement of Young Scientists from the Japan Society for the Promotion of Science (JSPS) (No. 16780036) and the Ohara Foundation. M. Ashfaq thanks JSPS for the award of Postdoctoral Fellowship for Foreign Researchers.",

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AU - Ashfaq, Muhammad

AU - Sonoda, Shoji

AU - Tsumuki, Hisaaki

N1 - Funding Information: This study was supported in part by a Grant-in-Aid for Encouragement of Young Scientists from the Japan Society for the Promotion of Science (JSPS) (No. 16780036) and the Ohara Foundation. M. Ashfaq thanks JSPS for the award of Postdoctoral Fellowship for Foreign Researchers.

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N2 - The cDNA of chitin synthase-1 gene of Plutella xylostella (PxCHS1) was characterized and expression patterns of the two splice variants PxCHS1A and PxCHS1B were investigated in various developmental stages and in major body tissues by RT-PCR and real-time quantitative PCR (qPCR). The PxCHS1 cDNA was 5461 bp in length with an open reading frame of 4701 bp that encoded a putative protein of 1567 amino acids with predicted molecular mass of 179 kDa. The two splice variants were expressed from the mutually exclusive exons which were same in size (177 bp) but showed only 66% identity at the nucleotide level. Both splice variants were expressed in all developmental stages. The qPCR data suggested an uneven expression of the two variants in the body where expression of PxCHS1A was 3.7-fold higher than that of PxCHS1B. The expression of PxCHS1A was 1.5-fold higher in the head than the body whereas in case of PxCHS1B the difference between head and body was 6.3-fold. Chlorfluazuron did not change the expression of PxCHS1 in larvae.

AB - The cDNA of chitin synthase-1 gene of Plutella xylostella (PxCHS1) was characterized and expression patterns of the two splice variants PxCHS1A and PxCHS1B were investigated in various developmental stages and in major body tissues by RT-PCR and real-time quantitative PCR (qPCR). The PxCHS1 cDNA was 5461 bp in length with an open reading frame of 4701 bp that encoded a putative protein of 1567 amino acids with predicted molecular mass of 179 kDa. The two splice variants were expressed from the mutually exclusive exons which were same in size (177 bp) but showed only 66% identity at the nucleotide level. Both splice variants were expressed in all developmental stages. The qPCR data suggested an uneven expression of the two variants in the body where expression of PxCHS1A was 3.7-fold higher than that of PxCHS1B. The expression of PxCHS1A was 1.5-fold higher in the head than the body whereas in case of PxCHS1B the difference between head and body was 6.3-fold. Chlorfluazuron did not change the expression of PxCHS1 in larvae.

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KW - Splice variants

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Developmental and tissue-specific expression of CHS1 from Plutella xylostella and its response to chlorfluazuron

Abstract

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