Developmental alteration of the expression and kinase activity of cyclin-dependent kinase 5 (Cdk5)/p35(nck5a) in the rat retina

Kazuyuki Hirooka, Kazuhito Tomizawa, Hideki Matsui, Masaaki Tokuda, Toshifumi Itano, Eiichi Hasegawa, Jerry H. Wang, Osamu Hatase

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Neuronal Cdc2-like kinase has been purified from the bovine brain as a proline-directed serine/threonine kinase. This kinase is a heterodimer of Cdk5 and p36(nck5a) and influences neuronal maturation or sprouting in the normal brain. In this study, we showed the expression of Cdk6/p35(nck5a) kinase in the developing rat retina. The expression of Cdk5 and p36(nck5a) increased between 1 week and 3 weeks after birth. These expression levels were most prominent from 2 weeks to 3 weeks after birth and decreased after 4 weeks. The developmental change of Cdk5/p35(nck5a) kinase activity coincided with those of the expression of p35(nck5a) and Cdk5. An immunohistochemical study showed that Cdk5 was expressed in the ganglion cells and in some cells in the inner nuclear layer at an early stage. With retinal development, Cdk5 was expressed in the inner plexiform layer also. In the adult, the expression of Cdk5 was restricted to the inner plexiform layer and to some cells in the inner nuclear layer. These changes of localization in the developing retina were very close to those of B-50/GAP-43. On the other hand, the expression of p35(nck5a) was restricted to the soma of the neuron in the developing retina. This subcellular localization in the developing retina agreed with that in the developing rat brain. The expression levels of Cdk5 and p35(nck5a) in retina of rats raised from fetus to 3 weeks after birth in darkness were 36% and 40% respectively, of the baseline for control rats. Moreover, the kinase activity in rats raised in darkness was lower than that in control rats. These data suggest that Cdk5/p35(nck5a) may play a role in neuronal plasticity in the developing rat retina.

Original languageEnglish
Pages (from-to)2478-2483
Number of pages6
JournalJournal of Neurochemistry
Volume67
Issue number6
Publication statusPublished - Dec 1996

Fingerprint

Cyclin-Dependent Kinase 5
Retina
Rats
Phosphotransferases
Rat control
Brain
Darkness
Parturition
GAP-43 Protein
neuronal Cdk5 activator (p25-p35)
Neuronal Plasticity
Protein-Serine-Threonine Kinases
Carisoprodol
Proline
Ganglia
Neurons
Plasticity
Fetus

Keywords

  • Development
  • GAP-43
  • Plasticity
  • Visual system

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

Hirooka, K., Tomizawa, K., Matsui, H., Tokuda, M., Itano, T., Hasegawa, E., ... Hatase, O. (1996). Developmental alteration of the expression and kinase activity of cyclin-dependent kinase 5 (Cdk5)/p35(nck5a) in the rat retina. Journal of Neurochemistry, 67(6), 2478-2483.

Developmental alteration of the expression and kinase activity of cyclin-dependent kinase 5 (Cdk5)/p35(nck5a) in the rat retina. / Hirooka, Kazuyuki; Tomizawa, Kazuhito; Matsui, Hideki; Tokuda, Masaaki; Itano, Toshifumi; Hasegawa, Eiichi; Wang, Jerry H.; Hatase, Osamu.

In: Journal of Neurochemistry, Vol. 67, No. 6, 12.1996, p. 2478-2483.

Research output: Contribution to journalArticle

Hirooka, K, Tomizawa, K, Matsui, H, Tokuda, M, Itano, T, Hasegawa, E, Wang, JH & Hatase, O 1996, 'Developmental alteration of the expression and kinase activity of cyclin-dependent kinase 5 (Cdk5)/p35(nck5a) in the rat retina', Journal of Neurochemistry, vol. 67, no. 6, pp. 2478-2483.
Hirooka, Kazuyuki ; Tomizawa, Kazuhito ; Matsui, Hideki ; Tokuda, Masaaki ; Itano, Toshifumi ; Hasegawa, Eiichi ; Wang, Jerry H. ; Hatase, Osamu. / Developmental alteration of the expression and kinase activity of cyclin-dependent kinase 5 (Cdk5)/p35(nck5a) in the rat retina. In: Journal of Neurochemistry. 1996 ; Vol. 67, No. 6. pp. 2478-2483.
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abstract = "Neuronal Cdc2-like kinase has been purified from the bovine brain as a proline-directed serine/threonine kinase. This kinase is a heterodimer of Cdk5 and p36(nck5a) and influences neuronal maturation or sprouting in the normal brain. In this study, we showed the expression of Cdk6/p35(nck5a) kinase in the developing rat retina. The expression of Cdk5 and p36(nck5a) increased between 1 week and 3 weeks after birth. These expression levels were most prominent from 2 weeks to 3 weeks after birth and decreased after 4 weeks. The developmental change of Cdk5/p35(nck5a) kinase activity coincided with those of the expression of p35(nck5a) and Cdk5. An immunohistochemical study showed that Cdk5 was expressed in the ganglion cells and in some cells in the inner nuclear layer at an early stage. With retinal development, Cdk5 was expressed in the inner plexiform layer also. In the adult, the expression of Cdk5 was restricted to the inner plexiform layer and to some cells in the inner nuclear layer. These changes of localization in the developing retina were very close to those of B-50/GAP-43. On the other hand, the expression of p35(nck5a) was restricted to the soma of the neuron in the developing retina. This subcellular localization in the developing retina agreed with that in the developing rat brain. The expression levels of Cdk5 and p35(nck5a) in retina of rats raised from fetus to 3 weeks after birth in darkness were 36{\%} and 40{\%} respectively, of the baseline for control rats. Moreover, the kinase activity in rats raised in darkness was lower than that in control rats. These data suggest that Cdk5/p35(nck5a) may play a role in neuronal plasticity in the developing rat retina.",
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