TY - JOUR
T1 - Developmental alteration and neuron-specific expression of bone morphogenetic protein-6 (BMP-6) mRNA in rodent brain
AU - Tomizawa, Kazuhito
AU - Matsui, Hideki
AU - Kondo, Eisaku
AU - Miyamoto, Kazuhiro
AU - Tokuda, Masaaki
AU - Itano, Toshifumi
AU - Nagahata, Shunichiro
AU - Akagi, Tadaatsu
AU - Hatase, Osamu
PY - 1995/1
Y1 - 1995/1
N2 - Bone morphogenetic proteins (BMPs) are a group of proteins which induce bone formation from mesenchymal cells. The existence of BMPs in the nervous system as well as in bone tissue has recently been reported. In this study, we show that BMP-6 is neuron-specific, and describe the temporal and spatial expression patterns of BMP-6 mRNA in the developing rat and gerbil brain. Northern blot analysis showed that the BMP-6 transcript level was specifically high from newborn to 3 weeks after birth compared with those in fetal and adult rats. In situ hybridization showed that most of the neurons possessed high levels of BMP-6 mRNA in the neonatal brain, while in the adult brain, BMP-6 mRNA level was significantly decreased in most of the neurons except those in hippocampus which retained high levels. Furthermore, to show that the BMP-6 expression was specific to neurons, we induced delayed neuronal cell death and compensative glial cell proliferation in the gerbil hippocampus by transient ischemia. Our findings collectively suggest that BMP-6 is neuron-specific and may play important roles in neuronal maturation and synapse formation.
AB - Bone morphogenetic proteins (BMPs) are a group of proteins which induce bone formation from mesenchymal cells. The existence of BMPs in the nervous system as well as in bone tissue has recently been reported. In this study, we show that BMP-6 is neuron-specific, and describe the temporal and spatial expression patterns of BMP-6 mRNA in the developing rat and gerbil brain. Northern blot analysis showed that the BMP-6 transcript level was specifically high from newborn to 3 weeks after birth compared with those in fetal and adult rats. In situ hybridization showed that most of the neurons possessed high levels of BMP-6 mRNA in the neonatal brain, while in the adult brain, BMP-6 mRNA level was significantly decreased in most of the neurons except those in hippocampus which retained high levels. Furthermore, to show that the BMP-6 expression was specific to neurons, we induced delayed neuronal cell death and compensative glial cell proliferation in the gerbil hippocampus by transient ischemia. Our findings collectively suggest that BMP-6 is neuron-specific and may play important roles in neuronal maturation and synapse formation.
KW - Bone morphogenetic protein
KW - Developing brain
KW - Ischemic brain
KW - Neuron
KW - Neurotrophic factor
KW - Transforming growth factor-β superfamily
UR - http://www.scopus.com/inward/record.url?scp=0028894722&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028894722&partnerID=8YFLogxK
U2 - 10.1016/0169-328X(94)00199-O
DO - 10.1016/0169-328X(94)00199-O
M3 - Article
C2 - 7707865
AN - SCOPUS:0028894722
VL - 28
SP - 122
EP - 128
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 1
ER -