Development of the O2-detoxification system during adaptation to air of submerged rice seedlings

Takashi Ushimaru, Mineo Shibasaka, Hideo Tsuji

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Changes in activities of enzymes and levels of antioxidant substrates involved in the O2--detoxification system in seedlings of rice (Oryza sativa L. cv. Yamabiko) in response to variations in the oxygen environment were studied. Activities of superoxide dismutase, ascorbate peroxidase, monodehydroascorbate reductase, dehydroascorbate reductase, glutathione reductase and catalase, expressed either on the basis of fresh weight of shoots or relative to levels of soluble protein were much lower in seedlings germinated under water for 6 days than in those germinated in air for the same period of time. When submerged seedlings were exposed to air, the activities of these six enzymes increased to or exceeded the levels in aerobically grown controls during 24 h of adaptation to air. Ascorbate and glutathione, which act as antioxidant substrates in the O2--detoxification system, were present in submerged seedlings at nearly the same levels as those found in aerobically grown controls. On exposure of submerged seedlings to air, the level of ascorbate in creased slightly, but the level of glutathione showed a rapid increase, reaching 7 times that in aerobically grown controls within 12 h of adaptation to air. Levels of all six antioxidative enzymes and of two substrates involved in the detoxification of the superoxide radical increased with increases in oxygen tension in the environment. Moreover, the development of this system consisted of two steps, namely, a rapid increase in the level of glutathione and a subsequent slow increase in the activities of antioxidative enzymes.

Original languageEnglish
Pages (from-to)1065-1071
Number of pages7
JournalPlant and Cell Physiology
Volume33
Issue number8
Publication statusPublished - Dec 1 1992

Keywords

  • Active oxygen
  • Antioxidant
  • Antioxidative enzyme
  • Oxygen deficiency
  • Rice (Oryza sativa)
  • Superoxide radical (O-2)

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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