Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens

Norihisa Yamamoto, Ryuji Kawahara, Yukihiro Akeda, Rathina Kumar Shanmugakani, Hisao Yoshida, Hideharu Hagiya, Naohiro Hara, Isao Nishi, Satomi Yukawa, Rumiko Asada, Yumi Sasaki, Kazuhiro Maeda, Noriko Sakamoto, Shigeyuki Hamada, Kazunori Tomono

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Background: Identification of carbapenemase-producing Enterobacteriaceae (CPE) in faecal specimens is challenging. This fact is particularly critical because low-level carbapenem-resistant organisms such as IMP-producing CPE are most prevalent in Japan. We developed a modified selective medium more suitable for IMP-type CPE. Methods: Fifteen reference CPE strains producing different types of β-lactamases were used to evaluate the commercially available CHROMagar KPC and chromID CARBA as well as the newly prepared MC-ECC medium (CHROMagar ECC supplemented with meropenem, cloxacillin, and ZnSO4) and M-ECC medium (CHROMagar ECC supplemented with meropenem and ZnSO4). A total of 1035 clinical samples were then examined to detect CPE using chromID CARBA and M-ECC medium. Results: All tested strains producing NDM-, KPC-, and OXA-48-carbapenemases were successfully cultured in the media employed. Although most of the IMP-positive strains did not grow in CHROMagar KPC, chromID CARBA, or MC-ECC, all tested strains grew on M-ECC. When faecal samples were applied to the media, M-ECC medium allowed the best growth of IMP-type CPE with a significantly higher sensitivity (99.3%) than that of chromID CARBA (13.9%). Conclusions: M-ECC medium was determined as the most favourable selective medium for the detection of IMP-type CPE as well as other types of CPE.

Original languageEnglish
Article number229
JournalBMC Infectious Diseases
Volume17
Issue number1
DOIs
Publication statusPublished - Mar 24 2017
Externally publishedYes

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Inosine Monophosphate
Enterobacteriaceae
meropenem
Cloxacillin
Carbapenems
carbapenemase
Japan
Growth

Keywords

  • Carbapenemase-producing Enterobacteriaceae
  • Faecal specimens
  • IMP
  • Selective culture medium

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens. / Yamamoto, Norihisa; Kawahara, Ryuji; Akeda, Yukihiro; Shanmugakani, Rathina Kumar; Yoshida, Hisao; Hagiya, Hideharu; Hara, Naohiro; Nishi, Isao; Yukawa, Satomi; Asada, Rumiko; Sasaki, Yumi; Maeda, Kazuhiro; Sakamoto, Noriko; Hamada, Shigeyuki; Tomono, Kazunori.

In: BMC Infectious Diseases, Vol. 17, No. 1, 229, 24.03.2017.

Research output: Contribution to journalArticle

Yamamoto, N, Kawahara, R, Akeda, Y, Shanmugakani, RK, Yoshida, H, Hagiya, H, Hara, N, Nishi, I, Yukawa, S, Asada, R, Sasaki, Y, Maeda, K, Sakamoto, N, Hamada, S & Tomono, K 2017, 'Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens', BMC Infectious Diseases, vol. 17, no. 1, 229. https://doi.org/10.1186/s12879-017-2312-1
Yamamoto, Norihisa ; Kawahara, Ryuji ; Akeda, Yukihiro ; Shanmugakani, Rathina Kumar ; Yoshida, Hisao ; Hagiya, Hideharu ; Hara, Naohiro ; Nishi, Isao ; Yukawa, Satomi ; Asada, Rumiko ; Sasaki, Yumi ; Maeda, Kazuhiro ; Sakamoto, Noriko ; Hamada, Shigeyuki ; Tomono, Kazunori. / Development of selective medium for IMP-type carbapenemase-producing Enterobacteriaceae in stool specimens. In: BMC Infectious Diseases. 2017 ; Vol. 17, No. 1.
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AU - Yamamoto, Norihisa

AU - Kawahara, Ryuji

AU - Akeda, Yukihiro

AU - Shanmugakani, Rathina Kumar

AU - Yoshida, Hisao

AU - Hagiya, Hideharu

AU - Hara, Naohiro

AU - Nishi, Isao

AU - Yukawa, Satomi

AU - Asada, Rumiko

AU - Sasaki, Yumi

AU - Maeda, Kazuhiro

AU - Sakamoto, Noriko

AU - Hamada, Shigeyuki

AU - Tomono, Kazunori

PY - 2017/3/24

Y1 - 2017/3/24

N2 - Background: Identification of carbapenemase-producing Enterobacteriaceae (CPE) in faecal specimens is challenging. This fact is particularly critical because low-level carbapenem-resistant organisms such as IMP-producing CPE are most prevalent in Japan. We developed a modified selective medium more suitable for IMP-type CPE. Methods: Fifteen reference CPE strains producing different types of β-lactamases were used to evaluate the commercially available CHROMagar KPC and chromID CARBA as well as the newly prepared MC-ECC medium (CHROMagar ECC supplemented with meropenem, cloxacillin, and ZnSO4) and M-ECC medium (CHROMagar ECC supplemented with meropenem and ZnSO4). A total of 1035 clinical samples were then examined to detect CPE using chromID CARBA and M-ECC medium. Results: All tested strains producing NDM-, KPC-, and OXA-48-carbapenemases were successfully cultured in the media employed. Although most of the IMP-positive strains did not grow in CHROMagar KPC, chromID CARBA, or MC-ECC, all tested strains grew on M-ECC. When faecal samples were applied to the media, M-ECC medium allowed the best growth of IMP-type CPE with a significantly higher sensitivity (99.3%) than that of chromID CARBA (13.9%). Conclusions: M-ECC medium was determined as the most favourable selective medium for the detection of IMP-type CPE as well as other types of CPE.

AB - Background: Identification of carbapenemase-producing Enterobacteriaceae (CPE) in faecal specimens is challenging. This fact is particularly critical because low-level carbapenem-resistant organisms such as IMP-producing CPE are most prevalent in Japan. We developed a modified selective medium more suitable for IMP-type CPE. Methods: Fifteen reference CPE strains producing different types of β-lactamases were used to evaluate the commercially available CHROMagar KPC and chromID CARBA as well as the newly prepared MC-ECC medium (CHROMagar ECC supplemented with meropenem, cloxacillin, and ZnSO4) and M-ECC medium (CHROMagar ECC supplemented with meropenem and ZnSO4). A total of 1035 clinical samples were then examined to detect CPE using chromID CARBA and M-ECC medium. Results: All tested strains producing NDM-, KPC-, and OXA-48-carbapenemases were successfully cultured in the media employed. Although most of the IMP-positive strains did not grow in CHROMagar KPC, chromID CARBA, or MC-ECC, all tested strains grew on M-ECC. When faecal samples were applied to the media, M-ECC medium allowed the best growth of IMP-type CPE with a significantly higher sensitivity (99.3%) than that of chromID CARBA (13.9%). Conclusions: M-ECC medium was determined as the most favourable selective medium for the detection of IMP-type CPE as well as other types of CPE.

KW - Carbapenemase-producing Enterobacteriaceae

KW - Faecal specimens

KW - IMP

KW - Selective culture medium

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