Development of observation system to investigate both intracellular calcium concentration and mechanical stimuli to mammalian embryos

Koji Matsuura, Koyo Watanabe, Mieko Kodama, Yuka Kuroda, Keiji Naruse

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Using an air-actuating device, we investigated the cellular response to mechanical stimuli (MS) in mouse blastocysts. Both MS and intracellular calcium concentration ([Ca2+]i) were quantified based on time-resolved confocal microscopy images in the polydimethylsiloxane (PDMS) microfluidic channels by deforming a 0.1-mm membrane. [Ca2+] i was measured in a stained mouse embryo with Fluo-4 AM using confocal fluorescence microscopy. We captured a z-series stack of sections encompassing the entire embryo. When translocation velocities of the embryo and shear stress were 40 μm/s and 0.01 dyne/cm2, respectively, a 10% increase in the sum of fluorescent intensities (FI) was observed. When blastocysts were compressed, FI also increased in response to the applied MS. Compressive force estimated from the shape of the blastocysts was approximately 0.5-2.0 μN according to a force deformation curve for the mouse embryo. The average FI and sum of FIs increased by a factor of 1.1-1.2 times compared with those observed before MS. The increase in the sum of FI indicated that enhancement of [Ca2+]i would be induced by these MS.

Original languageEnglish
Title of host publication2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"
PublisherIEEE Computer Society
Pages99-104
Number of pages6
ISBN (Print)9781457713613
DOIs
Publication statusPublished - 2011
Event22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation - Nagoya, Japan
Duration: Nov 6 2011Nov 9 2011

Publication series

Name2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"

Other

Other22nd Annual Symp. on Micro-Nano Mechatronics and Human Science, MHS 2011, Held Jointly with the Symp. on COE for Education and Research of Micro-Nano Mechatronics, Micro-Nano GCOE 2011, Symp. on Hyper Bio Assembler for 3D Cellular System Innovation
CountryJapan
CityNagoya
Period11/6/1111/9/11

ASJC Scopus subject areas

  • Artificial Intelligence
  • Mechanical Engineering

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  • Cite this

    Matsuura, K., Watanabe, K., Kodama, M., Kuroda, Y., & Naruse, K. (2011). Development of observation system to investigate both intracellular calcium concentration and mechanical stimuli to mammalian embryos. In 2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation" (pp. 99-104). [6102167] (2011 Int. Symp. on Micro-NanoMechatronics and Human Science, Symp. on "COE for Education and Research of Micro-Nano Mechatronics", Symposium on "Hyper Bio Assembler for 3D Cellular System Innovation"). IEEE Computer Society. https://doi.org/10.1109/MHS.2011.6102167