Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles

A. Tsukamoto, K. Saitoh, D. Suzuki, N. Sugita, Y. Seki, A. Kandori, Keiji Tsukada, Y. Sugiura, S. Hamaoka, H. Kuma, N. Hamasaki, K. Enpuku

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

We developed a prototype magnetic immunoassay system using a high temperature superconductor (HTS) superconducting quantum interference device (SQUID) to investigate the performance and usability of the magnetic immunoassay. The system is designed to measure multiple samples and liquid samples, and it can work in an unshielded environment at a medical facility. To reduce the disturbance from environmental noise, the SQUID and samples are covered with three-layers of permalloy magnetic shield. The SQUID and magnetic shield are set in an aluminum box which acts as an RF shield. A gradiometer with a 5 × 10 mm pickup coil, which is cooled by liquid nitrogen through a sapphire/Cu rod, is used as a sensor. We also developed a nonmagnetic sample disk with 12 reaction cells and examined 12 samples in one measurement sequence. The measurement process is controlled by a computer, which perform data averaging. Fe3O4 nanoparticles with a 25-nm diameter were used as test samples. After applying a magnetic field of about 0.1 T, we measured the remanent magnetic field from the Fe3O4 nanoparticles. The present system could detect 30 pg of Fe3O 4 nanoparticles. This result was obtained by averaging 100 trials under an unshielded laboratory environment. The measurement time for 100 trials was only 100 s.

Original languageEnglish
Pages (from-to)656-659
Number of pages4
JournalIEEE Transactions on Applied Superconductivity
Volume15
Issue number2 PART I
DOIs
Publication statusPublished - Jun 2005

Fingerprint

immunoassay
High temperature superconductors
SQUIDs
Biological systems
high temperature superconductors
Nanoparticles
interference
nanoparticles
Magnetic fields
Aluminum Oxide
Pickups
Liquid nitrogen
Time measurement
Aluminum
Sapphire
gradiometers
sensors
Sensors
Permalloys (trademark)
Liquids

Keywords

  • FeO nanoparticles
  • High-T SQUID
  • Magnetic immunoassay
  • Remanence

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Physics and Astronomy (miscellaneous)

Cite this

Tsukamoto, A., Saitoh, K., Suzuki, D., Sugita, N., Seki, Y., Kandori, A., ... Enpuku, K. (2005). Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles. IEEE Transactions on Applied Superconductivity, 15(2 PART I), 656-659. https://doi.org/10.1109/TASC.2005.849988

Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles. / Tsukamoto, A.; Saitoh, K.; Suzuki, D.; Sugita, N.; Seki, Y.; Kandori, A.; Tsukada, Keiji; Sugiura, Y.; Hamaoka, S.; Kuma, H.; Hamasaki, N.; Enpuku, K.

In: IEEE Transactions on Applied Superconductivity, Vol. 15, No. 2 PART I, 06.2005, p. 656-659.

Research output: Contribution to journalArticle

Tsukamoto, A, Saitoh, K, Suzuki, D, Sugita, N, Seki, Y, Kandori, A, Tsukada, K, Sugiura, Y, Hamaoka, S, Kuma, H, Hamasaki, N & Enpuku, K 2005, 'Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles', IEEE Transactions on Applied Superconductivity, vol. 15, no. 2 PART I, pp. 656-659. https://doi.org/10.1109/TASC.2005.849988
Tsukamoto, A. ; Saitoh, K. ; Suzuki, D. ; Sugita, N. ; Seki, Y. ; Kandori, A. ; Tsukada, Keiji ; Sugiura, Y. ; Hamaoka, S. ; Kuma, H. ; Hamasaki, N. ; Enpuku, K. / Development of multisample biological immunoassay system using HTS SQUID and magnetic nanoparticles. In: IEEE Transactions on Applied Superconductivity. 2005 ; Vol. 15, No. 2 PART I. pp. 656-659.
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