Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae)

Koki R. Katsuhara; Naoyuki Nakahama; Taketo Komura; Masaya Kato; Yuko Miyazaki; Yuji Isagi; Motomi Ito; Atushi Ushimaru

doi:10.1266/ggs.18-00058

Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae)

Koki R. Katsuhara, Naoyuki Nakahama, Taketo Komura, Masaya Kato, Yuko Miyazaki, Yuji Isagi, Motomi Ito, Atushi Ushimaru

Research output: Contribution to journal › Article › peer-review

Abstract

Commelina communis f. ciliata (Commelinaceae), a newly distinguished taxon, is an annual andromonoecious herb exhibiting a mixed mating system, the details of which remain unclear. We developed microsatellite markers for use in exploring the evolution of andromonoecy and mixed mating in the species. Fifteen microsatellite loci were developed using next-generation sequencing. The primer sets were used to evaluate 65 C. communis f. ciliata individuals from three populations in Japan; we found 1–13 alleles per locus and the expected heterozygosity ranged from 0.00 to 0.76. The markers are potentially useful to examine intra-and inter-species genetic structure and the mixed mating strategy of Commelina species via paternity analysis.

Original language	English
Pages (from-to)	133-138
Number of pages	6
Journal	Genes and Genetic Systems
Volume	94
Issue number	3
DOIs	https://doi.org/10.1266/ggs.18-00058
Publication status	Published - 2019

Keywords

Andromonoecy
Commelina
Next-generation sequencing
Paternity analysis

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1266/ggs.18-00058

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Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae). / Katsuhara, Koki R.; Nakahama, Naoyuki; Komura, Taketo; Kato, Masaya; Miyazaki, Yuko; Isagi, Yuji; Ito, Motomi; Ushimaru, Atushi.

In: Genes and Genetic Systems, Vol. 94, No. 3, 2019, p. 133-138.

Research output: Contribution to journal › Article › peer-review

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title = "Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae)",

abstract = "Commelina communis f. ciliata (Commelinaceae), a newly distinguished taxon, is an annual andromonoecious herb exhibiting a mixed mating system, the details of which remain unclear. We developed microsatellite markers for use in exploring the evolution of andromonoecy and mixed mating in the species. Fifteen microsatellite loci were developed using next-generation sequencing. The primer sets were used to evaluate 65 C. communis f. ciliata individuals from three populations in Japan; we found 1–13 alleles per locus and the expected heterozygosity ranged from 0.00 to 0.76. The markers are potentially useful to examine intra-and inter-species genetic structure and the mixed mating strategy of Commelina species via paternity analysis.",

keywords = "Andromonoecy, Commelina, Next-generation sequencing, Paternity analysis",

author = "Katsuhara, {Koki R.} and Naoyuki Nakahama and Taketo Komura and Masaya Kato and Yuko Miyazaki and Yuji Isagi and Motomi Ito and Atushi Ushimaru",

note = "Funding Information: The authors thank S. Kurata and N. Ishikawa for help with the experiments. We also thank M. Yokogawa and Y. Yaida for help with preparing the voucher specimens and sampling, respectively. This work was supported by Grants-in-Aid for Scientific Research Programs (nos. 16K07517 and 17J01902) from the Japan Society for the Promotion of Science. Publisher Copyright: {\textcopyright} 2019, Genetics Society of Japan. All rights reserved.",

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doi = "10.1266/ggs.18-00058",

language = "English",

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AU - Nakahama, Naoyuki

AU - Komura, Taketo

AU - Kato, Masaya

AU - Miyazaki, Yuko

AU - Isagi, Yuji

AU - Ito, Motomi

AU - Ushimaru, Atushi

N1 - Funding Information: The authors thank S. Kurata and N. Ishikawa for help with the experiments. We also thank M. Yokogawa and Y. Yaida for help with preparing the voucher specimens and sampling, respectively. This work was supported by Grants-in-Aid for Scientific Research Programs (nos. 16K07517 and 17J01902) from the Japan Society for the Promotion of Science. Publisher Copyright: © 2019, Genetics Society of Japan. All rights reserved.

PY - 2019

Y1 - 2019

N2 - Commelina communis f. ciliata (Commelinaceae), a newly distinguished taxon, is an annual andromonoecious herb exhibiting a mixed mating system, the details of which remain unclear. We developed microsatellite markers for use in exploring the evolution of andromonoecy and mixed mating in the species. Fifteen microsatellite loci were developed using next-generation sequencing. The primer sets were used to evaluate 65 C. communis f. ciliata individuals from three populations in Japan; we found 1–13 alleles per locus and the expected heterozygosity ranged from 0.00 to 0.76. The markers are potentially useful to examine intra-and inter-species genetic structure and the mixed mating strategy of Commelina species via paternity analysis.

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Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae)

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Keywords

ASJC Scopus subject areas

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