Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae)

Koki R. Katsuhara; Naoyuki Nakahama; Taketo Komura; Masaya Kato; Yuko Miyazaki; Yuji Isagi; Motomi Ito; Atushi Ushimaru

doi:10.1266/ggs.18-00058

Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae)

Koki R. Katsuhara, Naoyuki Nakahama, Taketo Komura, Masaya Kato, Yuko Miyazaki, Yuji Isagi, Motomi Ito, Atushi Ushimaru

Research output: Contribution to journal › Article

Abstract

Commelina communis f. ciliata (Commelinaceae), a newly distinguished taxon, is an annual andromonoecious herb exhibiting a mixed mating system, the details of which remain unclear. We developed microsatellite markers for use in exploring the evolution of andromonoecy and mixed mating in the species. Fifteen microsatellite loci were developed using next-generation sequencing. The primer sets were used to evaluate 65 C. communis f. ciliata individuals from three populations in Japan; we found 1–13 alleles per locus and the expected heterozygosity ranged from 0.00 to 0.76. The markers are potentially useful to examine intra-and inter-species genetic structure and the mixed mating strategy of Commelina species via paternity analysis.

Original language	English
Pages (from-to)	133-138
Number of pages	6
Journal	Genes and Genetic Systems
Volume	94
Issue number	3
DOIs	https://doi.org/10.1266/ggs.18-00058
Publication status	Published - Jan 1 2019

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Keywords

Andromonoecy
Commelina
Next-generation sequencing
Paternity analysis

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Katsuhara, K. R., Nakahama, N., Komura, T., Kato, M., Miyazaki, Y., Isagi, Y., Ito, M., & Ushimaru, A. (2019). Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae). Genes and Genetic Systems, 94(3), 133-138. https://doi.org/10.1266/ggs.18-00058

Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae). / Katsuhara, Koki R.; Nakahama, Naoyuki; Komura, Taketo; Kato, Masaya; Miyazaki, Yuko; Isagi, Yuji; Ito, Motomi; Ushimaru, Atushi.

In: Genes and Genetic Systems, Vol. 94, No. 3, 01.01.2019, p. 133-138.

Research output: Contribution to journal › Article

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10.1266/ggs.18-00058