Development of microsatellite markers for the annual andromonoecious herb Commelina communis f. Ciliata (commelinaceae)

Koki R. Katsuhara, Naoyuki Nakahama, Taketo Komura, Masaya Kato, Yuko Miyazaki, Yuji Isagi, Motomi Ito, Atushi Ushimaru

Research output: Contribution to journalArticle

Abstract

Commelina communis f. ciliata (Commelinaceae), a newly distinguished taxon, is an annual andromonoecious herb exhibiting a mixed mating system, the details of which remain unclear. We developed microsatellite markers for use in exploring the evolution of andromonoecy and mixed mating in the species. Fifteen microsatellite loci were developed using next-generation sequencing. The primer sets were used to evaluate 65 C. communis f. ciliata individuals from three populations in Japan; we found 1–13 alleles per locus and the expected heterozygosity ranged from 0.00 to 0.76. The markers are potentially useful to examine intra-and inter-species genetic structure and the mixed mating strategy of Commelina species via paternity analysis.

Original languageEnglish
Pages (from-to)133-138
Number of pages6
JournalGenes and Genetic Systems
Volume94
Issue number3
DOIs
Publication statusPublished - 2019

Keywords

  • Andromonoecy
  • Commelina
  • Next-generation sequencing
  • Paternity analysis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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