Development of mammalian cell-enclosing subsieve-size agarose capsules (

Shinji Sakai, Kenji Kawabata, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

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Abstract

Agarose capsules were prepared using a droplet breakup method in a coflowing stream. Subsieve-size capsules 76±9 μm in diameter were obtained by extruding 4 wt% agarose solution from a needle (300 μm inner diameter) at a velocity of 1.2 cm/s into an ambient liquid paraffin flow of 20.8 cm/s. Increasing the flow rate of the liquid paraffin and decreasing that of the agarose solution resulted in a decreased resultant capsule diameter. Reduction in diameter from several hundred micrometers to subsieve-size (

Original languageEnglish
Pages (from-to)4786-4792
Number of pages7
JournalBiomaterials
Volume26
Issue number23
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

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Keywords

  • Cell encapsulation
  • Cell viability
  • Microcapsule
  • Microencapsulation
  • Polysaccharide
  • Transplantation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

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