Development of mammalian cell-enclosing subsieve-size agarose capsules (

Shinji Sakai, Kenji Kawabata, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Agarose capsules were prepared using a droplet breakup method in a coflowing stream. Subsieve-size capsules 76±9 μm in diameter were obtained by extruding 4 wt% agarose solution from a needle (300 μm inner diameter) at a velocity of 1.2 cm/s into an ambient liquid paraffin flow of 20.8 cm/s. Increasing the flow rate of the liquid paraffin and decreasing that of the agarose solution resulted in a decreased resultant capsule diameter. Reduction in diameter from several hundred micrometers to subsieve-size (

Original languageEnglish
Pages (from-to)4786-4792
Number of pages7
JournalBiomaterials
Volume26
Issue number23
DOIs
Publication statusPublished - Aug 2005
Externally publishedYes

Fingerprint

Sepharose
Paraffins
Capsules
Mineral Oil
Cells
Liquids
Needles
Flow rate

Keywords

  • Cell encapsulation
  • Cell viability
  • Microcapsule
  • Microencapsulation
  • Polysaccharide
  • Transplantation

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering

Cite this

Development of mammalian cell-enclosing subsieve-size agarose capsules (. / Sakai, Shinji; Kawabata, Kenji; Ono, Tsutomu; Ijima, Hiroyuki; Kawakami, Koei.

In: Biomaterials, Vol. 26, No. 23, 08.2005, p. 4786-4792.

Research output: Contribution to journalArticle

Sakai, Shinji ; Kawabata, Kenji ; Ono, Tsutomu ; Ijima, Hiroyuki ; Kawakami, Koei. / Development of mammalian cell-enclosing subsieve-size agarose capsules (. In: Biomaterials. 2005 ; Vol. 26, No. 23. pp. 4786-4792.
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