Development of liposome-based mimics of superoxide dismutase and peroxidase based on the "LIPOzyme" concept

Hiroshi Umakoshi, Kengo Morimoto, Naoto Yasuda, Toshinori Shimanouchi, Ryoichi Kuboi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

An antioxidative liposome catalyst, LIPOzyme, that mimics both superoxide dismutase (SOD) and peroxidase (POD)-like activities has been developed by using liposomes modified with simple ligands (dodecanoyl-histidine, Dodec-His) and metal ions (Mn). The SOD-like activity is dependent on the stability of the ligand-metal complex on the liposome membrane, with the value being higher for the DPPC liposome and at a higher pH. The POD-like activity was found to be maximal in the case of DMPC liposome, in which the ligand-metal complex is inserted more deeply into the membrane. It was thus shown that liposome modified with simple ligands can exhibit different enzyme-like activities depending on the characteristics of the liposome membrane.

Original languageEnglish
Pages (from-to)59-63
Number of pages5
JournalJournal of Biotechnology
Volume147
Issue number1
DOIs
Publication statusPublished - May 2010
Externally publishedYes

Fingerprint

Liposomes
Peroxidase
Superoxide Dismutase
Ligands
Coordination Complexes
Metal complexes
Membranes
Dimyristoylphosphatidylcholine
Lipozyme
Histidine
Metal ions
Enzymes
Metals
Ions
Catalysts

Keywords

  • Antioxidative enzymes
  • Enzyme mimics
  • Liposome
  • LIPOzyme
  • Membrane stress biotechnology
  • Membranome

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Medicine(all)

Cite this

Development of liposome-based mimics of superoxide dismutase and peroxidase based on the "LIPOzyme" concept. / Umakoshi, Hiroshi; Morimoto, Kengo; Yasuda, Naoto; Shimanouchi, Toshinori; Kuboi, Ryoichi.

In: Journal of Biotechnology, Vol. 147, No. 1, 05.2010, p. 59-63.

Research output: Contribution to journalArticle

Umakoshi, Hiroshi ; Morimoto, Kengo ; Yasuda, Naoto ; Shimanouchi, Toshinori ; Kuboi, Ryoichi. / Development of liposome-based mimics of superoxide dismutase and peroxidase based on the "LIPOzyme" concept. In: Journal of Biotechnology. 2010 ; Vol. 147, No. 1. pp. 59-63.
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