Abstract
An antioxidative liposome catalyst, LIPOzyme, that mimics both superoxide dismutase (SOD) and peroxidase (POD)-like activities has been developed by using liposomes modified with simple ligands (dodecanoyl-histidine, Dodec-His) and metal ions (Mn). The SOD-like activity is dependent on the stability of the ligand-metal complex on the liposome membrane, with the value being higher for the DPPC liposome and at a higher pH. The POD-like activity was found to be maximal in the case of DMPC liposome, in which the ligand-metal complex is inserted more deeply into the membrane. It was thus shown that liposome modified with simple ligands can exhibit different enzyme-like activities depending on the characteristics of the liposome membrane.
| Original language | English |
|---|---|
| Pages (from-to) | 59-63 |
| Number of pages | 5 |
| Journal | Journal of biotechnology |
| Volume | 147 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - May 1 2010 |
| Externally published | Yes |
Keywords
- Antioxidative enzymes
- Enzyme mimics
- LIPOzyme
- Liposome
- Membrane stress biotechnology
- Membranome
ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
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Cite this
Umakoshi, H., Morimoto, K., Yasuda, N., Shimanouchi, T., & Kuboi, R. (2010). Development of liposome-based mimics of superoxide dismutase and peroxidase based on the "LIPOzyme" concept. Journal of biotechnology, 147(1), 59-63. https://doi.org/10.1016/j.jbiotec.2010.02.013