TY - JOUR
T1 - Development of liposome-based mimics of superoxide dismutase and peroxidase based on the "LIPOzyme" concept
AU - Umakoshi, Hiroshi
AU - Morimoto, Kengo
AU - Yasuda, Naoto
AU - Shimanouchi, Toshinori
AU - Kuboi, Ryoichi
N1 - Funding Information:
The fundamental concept of this study was supported by the Research Group of “Membrane Stress Biotechnology.” It was supported in part by a Grant-in-Aid for Scientific Research (Nos. 17656268, 19656203, 19656220, 20360350, and 21246121 ) from the Ministry of Education, Science, Sports, and Culture of Japan , a grant from the 21st Century COE program “Creation of Integrated EcoChemistry” and the Global COE program “Bio-Environmental Chemistry” of the Japan Society for the Promotion of Science (JSPS). The authors are grateful to the Research Center for Solar Energy Chemistry of Osaka University and the Gas hydrate Analyzing System of Osaka University.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/5
Y1 - 2010/5
N2 - An antioxidative liposome catalyst, LIPOzyme, that mimics both superoxide dismutase (SOD) and peroxidase (POD)-like activities has been developed by using liposomes modified with simple ligands (dodecanoyl-histidine, Dodec-His) and metal ions (Mn). The SOD-like activity is dependent on the stability of the ligand-metal complex on the liposome membrane, with the value being higher for the DPPC liposome and at a higher pH. The POD-like activity was found to be maximal in the case of DMPC liposome, in which the ligand-metal complex is inserted more deeply into the membrane. It was thus shown that liposome modified with simple ligands can exhibit different enzyme-like activities depending on the characteristics of the liposome membrane.
AB - An antioxidative liposome catalyst, LIPOzyme, that mimics both superoxide dismutase (SOD) and peroxidase (POD)-like activities has been developed by using liposomes modified with simple ligands (dodecanoyl-histidine, Dodec-His) and metal ions (Mn). The SOD-like activity is dependent on the stability of the ligand-metal complex on the liposome membrane, with the value being higher for the DPPC liposome and at a higher pH. The POD-like activity was found to be maximal in the case of DMPC liposome, in which the ligand-metal complex is inserted more deeply into the membrane. It was thus shown that liposome modified with simple ligands can exhibit different enzyme-like activities depending on the characteristics of the liposome membrane.
KW - Antioxidative enzymes
KW - Enzyme mimics
KW - LIPOzyme
KW - Liposome
KW - Membrane stress biotechnology
KW - Membranome
UR - http://www.scopus.com/inward/record.url?scp=77951978160&partnerID=8YFLogxK
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U2 - 10.1016/j.jbiotec.2010.02.013
DO - 10.1016/j.jbiotec.2010.02.013
M3 - Article
C2 - 20188129
AN - SCOPUS:77951978160
VL - 147
SP - 59
EP - 63
JO - Journal of Biotechnology
JF - Journal of Biotechnology
SN - 0168-1656
IS - 1
ER -