Development of injectable O-15 oxygen and estimation of OEF in a transient ischemia–reperfusion rat model

Takashi Temma; Yasuhiro Magata; Hidehiro Iida; Mikako Ogawa; Takahiro Mukai; Yasuhiko Iida; Masashi Ueda; Junji Konishi; Hideo Saji

doi:10.1016/j.ics.2003.12.067

Development of injectable O-15 oxygen and estimation of OEF in a transient ischemia–reperfusion rat model

Takashi Temma, Yasuhiro Magata, Hidehiro Iida, Mikako Ogawa, Takahiro Mukai, Yasuhiko Iida, Masashi Ueda, Junji Konishi, Hideo Saji

Research output: Contribution to journal › Article › peer-review

Abstract

In the basic research about cerebral perfusion disorders, a new method for measurement of oxygen extraction fraction (OEF) and cerebral metabolic rate for oxygen (CMRO₂) has been desired. Although O-15-labeled gas inhalation method is performed in clinical studies, application of the inhalation method to small animals requires too many intensive procedures. Therefore, this study investigated a new method of assessing OEF in small animals using intravenously injectable oxygen (injectable ¹⁵O-O₂). Moreover, OEF after transient ischemia–reperfusion was estimated to validate the usefulness of the method. Injectable ¹⁵O-O₂, 72 MBq/ml of radioactivity, was obtained after ¹⁵O-O₂ gas circulation into the artificial lung. OEF after injection of injectable ¹⁵O-O₂ was calculated using the same equation as that applied to the bolus inhalation method. OEF value obtained by injectable ¹⁵O-O₂ was well in accordance with that evaluated by arterial-venous difference of oxygen concentration. Furthermore, misery perfusion was depicted in the lesioned hemisphere after transient ischemia–reperfusion. These results indicated that this method is useful for the estimation of OEF and CMRO₂ in small animals using an animal positron emission tomography (PET) system and may accelerate the basic research of cerebral perfusion diseases.

Original language	English
Pages (from-to)	197-201
Number of pages	5
Journal	International Congress Series
Volume	1264
Issue number	C
DOIs	https://doi.org/10.1016/j.ics.2003.12.067
Publication status	Published - Mar 1 2004
Externally published	Yes

Keywords

Ischemia
OEF
Oxygen-15
Positron
Rat

ASJC Scopus subject areas

Medicine(all)

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10.1016/j.ics.2003.12.067

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title = "Development of injectable O-15 oxygen and estimation of OEF in a transient ischemia–reperfusion rat model",

abstract = "In the basic research about cerebral perfusion disorders, a new method for measurement of oxygen extraction fraction (OEF) and cerebral metabolic rate for oxygen (CMRO2) has been desired. Although O-15-labeled gas inhalation method is performed in clinical studies, application of the inhalation method to small animals requires too many intensive procedures. Therefore, this study investigated a new method of assessing OEF in small animals using intravenously injectable oxygen (injectable 15O-O2). Moreover, OEF after transient ischemia–reperfusion was estimated to validate the usefulness of the method. Injectable 15O-O2, 72 MBq/ml of radioactivity, was obtained after 15O-O2 gas circulation into the artificial lung. OEF after injection of injectable 15O-O2 was calculated using the same equation as that applied to the bolus inhalation method. OEF value obtained by injectable 15O-O2 was well in accordance with that evaluated by arterial-venous difference of oxygen concentration. Furthermore, misery perfusion was depicted in the lesioned hemisphere after transient ischemia–reperfusion. These results indicated that this method is useful for the estimation of OEF and CMRO2 in small animals using an animal positron emission tomography (PET) system and may accelerate the basic research of cerebral perfusion diseases.",

keywords = "Ischemia, OEF, Oxygen-15, Positron, Rat",

author = "Takashi Temma and Yasuhiro Magata and Hidehiro Iida and Mikako Ogawa and Takahiro Mukai and Yasuhiko Iida and Masashi Ueda and Junji Konishi and Hideo Saji",

year = "2004",

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doi = "10.1016/j.ics.2003.12.067",

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T1 - Development of injectable O-15 oxygen and estimation of OEF in a transient ischemia–reperfusion rat model

AU - Temma, Takashi

AU - Magata, Yasuhiro

AU - Iida, Hidehiro

AU - Ogawa, Mikako

AU - Mukai, Takahiro

AU - Iida, Yasuhiko

AU - Ueda, Masashi

AU - Konishi, Junji

AU - Saji, Hideo

PY - 2004/3/1

Y1 - 2004/3/1

N2 - In the basic research about cerebral perfusion disorders, a new method for measurement of oxygen extraction fraction (OEF) and cerebral metabolic rate for oxygen (CMRO2) has been desired. Although O-15-labeled gas inhalation method is performed in clinical studies, application of the inhalation method to small animals requires too many intensive procedures. Therefore, this study investigated a new method of assessing OEF in small animals using intravenously injectable oxygen (injectable 15O-O2). Moreover, OEF after transient ischemia–reperfusion was estimated to validate the usefulness of the method. Injectable 15O-O2, 72 MBq/ml of radioactivity, was obtained after 15O-O2 gas circulation into the artificial lung. OEF after injection of injectable 15O-O2 was calculated using the same equation as that applied to the bolus inhalation method. OEF value obtained by injectable 15O-O2 was well in accordance with that evaluated by arterial-venous difference of oxygen concentration. Furthermore, misery perfusion was depicted in the lesioned hemisphere after transient ischemia–reperfusion. These results indicated that this method is useful for the estimation of OEF and CMRO2 in small animals using an animal positron emission tomography (PET) system and may accelerate the basic research of cerebral perfusion diseases.

AB - In the basic research about cerebral perfusion disorders, a new method for measurement of oxygen extraction fraction (OEF) and cerebral metabolic rate for oxygen (CMRO2) has been desired. Although O-15-labeled gas inhalation method is performed in clinical studies, application of the inhalation method to small animals requires too many intensive procedures. Therefore, this study investigated a new method of assessing OEF in small animals using intravenously injectable oxygen (injectable 15O-O2). Moreover, OEF after transient ischemia–reperfusion was estimated to validate the usefulness of the method. Injectable 15O-O2, 72 MBq/ml of radioactivity, was obtained after 15O-O2 gas circulation into the artificial lung. OEF after injection of injectable 15O-O2 was calculated using the same equation as that applied to the bolus inhalation method. OEF value obtained by injectable 15O-O2 was well in accordance with that evaluated by arterial-venous difference of oxygen concentration. Furthermore, misery perfusion was depicted in the lesioned hemisphere after transient ischemia–reperfusion. These results indicated that this method is useful for the estimation of OEF and CMRO2 in small animals using an animal positron emission tomography (PET) system and may accelerate the basic research of cerebral perfusion diseases.

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KW - Oxygen-15

KW - Positron

KW - Rat

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Development of injectable O-15 oxygen and estimation of OEF in a transient ischemia–reperfusion rat model

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