Development of injectable O-15 oxygen and estimation of OEF in a transient ischemia–reperfusion rat model

Takashi Temma, Yasuhiro Magata, Hidehiro Iida, Mikako Ogawa, Takahiro Mukai, Yasuhiko Iida, Masashi Ueda, Junji Konishi, Hideo Saji

Research output: Contribution to journalArticle

Abstract

In the basic research about cerebral perfusion disorders, a new method for measurement of oxygen extraction fraction (OEF) and cerebral metabolic rate for oxygen (CMRO2) has been desired. Although O-15-labeled gas inhalation method is performed in clinical studies, application of the inhalation method to small animals requires too many intensive procedures. Therefore, this study investigated a new method of assessing OEF in small animals using intravenously injectable oxygen (injectable 15O-O2). Moreover, OEF after transient ischemia–reperfusion was estimated to validate the usefulness of the method. Injectable 15O-O2, 72 MBq/ml of radioactivity, was obtained after 15O-O2 gas circulation into the artificial lung. OEF after injection of injectable 15O-O2 was calculated using the same equation as that applied to the bolus inhalation method. OEF value obtained by injectable 15O-O2 was well in accordance with that evaluated by arterial-venous difference of oxygen concentration. Furthermore, misery perfusion was depicted in the lesioned hemisphere after transient ischemia–reperfusion. These results indicated that this method is useful for the estimation of OEF and CMRO2 in small animals using an animal positron emission tomography (PET) system and may accelerate the basic research of cerebral perfusion diseases.

Original languageEnglish
Pages (from-to)197-201
Number of pages5
JournalInternational Congress Series
Volume1264
Issue numberC
DOIs
Publication statusPublished - Mar 1 2004
Externally publishedYes

Keywords

  • Ischemia
  • OEF
  • Oxygen-15
  • Positron
  • Rat

ASJC Scopus subject areas

  • Medicine(all)

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