Development of co-culture system of hepatocytes with bone marrow cells for expression and maintenance of hepatic functions

S. Murakami, Hiroyuki Ijima, Tsutomu Ono, K. Kawakami

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In this study, a co-culture system of hepatocytes and bone marrow cells (BMCs) was developed and characterized for the expression and maintenance of ammonia metabolism and albumin secretion activities. A culture medium supplemented with epidermal growth factor, insulin, L-proline, hydrocortisone and 20 % (v/v) heat-inactivated fetal bovine serum was developed. In addition to adhesive bone marrow cells, the co-existence of non-adhesive bone marrow cells was effective in expressing liver-specific functions for at least 3 weeks. On the other hand, experiments with Transwell in which cultured cells were separated by a semi-permeable membrane, suggested that soluble factors secreted by BMCs are the key components in the functional enhancement of cells. Furthermore, direct contact between hepatocytes and BMCs enhanced the formation of spheroids and the expression of liver specific functions. These results indicate that this co-culture system is promising in, for example, bioartificial liver, regenerative medicine, and liver function simulator applications.

Original languageEnglish
Pages (from-to)118-126
Number of pages9
JournalInternational Journal of Artificial Organs
Volume27
Issue number2
Publication statusPublished - Feb 2004
Externally publishedYes

Fingerprint

Coculture Techniques
Cell culture
Bone Marrow Cells
Hepatocytes
Bone
Liver
Cells
Maintenance
Artificial Liver
Cortisol
Regenerative Medicine
Insulin
Cellular Structures
Ammonia
Epidermal Growth Factor
Proline
Metabolism
Adhesives
Culture Media
Hydrocortisone

Keywords

  • Albumin secretion
  • Ammonia metabolism
  • Bone marrow cells
  • Co-culture
  • Heat-inactivated fetal bovine serum
  • Hepatocyte
  • Non-adhesive bone marrow cells
  • Soluble factors
  • Spheroid
  • Transwell

ASJC Scopus subject areas

  • Biophysics

Cite this

Development of co-culture system of hepatocytes with bone marrow cells for expression and maintenance of hepatic functions. / Murakami, S.; Ijima, Hiroyuki; Ono, Tsutomu; Kawakami, K.

In: International Journal of Artificial Organs, Vol. 27, No. 2, 02.2004, p. 118-126.

Research output: Contribution to journalArticle

@article{26d2c46427ce4919a5bf1db03882d79c,
title = "Development of co-culture system of hepatocytes with bone marrow cells for expression and maintenance of hepatic functions",
abstract = "In this study, a co-culture system of hepatocytes and bone marrow cells (BMCs) was developed and characterized for the expression and maintenance of ammonia metabolism and albumin secretion activities. A culture medium supplemented with epidermal growth factor, insulin, L-proline, hydrocortisone and 20 {\%} (v/v) heat-inactivated fetal bovine serum was developed. In addition to adhesive bone marrow cells, the co-existence of non-adhesive bone marrow cells was effective in expressing liver-specific functions for at least 3 weeks. On the other hand, experiments with Transwell in which cultured cells were separated by a semi-permeable membrane, suggested that soluble factors secreted by BMCs are the key components in the functional enhancement of cells. Furthermore, direct contact between hepatocytes and BMCs enhanced the formation of spheroids and the expression of liver specific functions. These results indicate that this co-culture system is promising in, for example, bioartificial liver, regenerative medicine, and liver function simulator applications.",
keywords = "Albumin secretion, Ammonia metabolism, Bone marrow cells, Co-culture, Heat-inactivated fetal bovine serum, Hepatocyte, Non-adhesive bone marrow cells, Soluble factors, Spheroid, Transwell",
author = "S. Murakami and Hiroyuki Ijima and Tsutomu Ono and K. Kawakami",
year = "2004",
month = "2",
language = "English",
volume = "27",
pages = "118--126",
journal = "International Journal of Artificial Organs",
issn = "0391-3988",
publisher = "Wichtig Publishing",
number = "2",

}

TY - JOUR

T1 - Development of co-culture system of hepatocytes with bone marrow cells for expression and maintenance of hepatic functions

AU - Murakami, S.

AU - Ijima, Hiroyuki

AU - Ono, Tsutomu

AU - Kawakami, K.

PY - 2004/2

Y1 - 2004/2

N2 - In this study, a co-culture system of hepatocytes and bone marrow cells (BMCs) was developed and characterized for the expression and maintenance of ammonia metabolism and albumin secretion activities. A culture medium supplemented with epidermal growth factor, insulin, L-proline, hydrocortisone and 20 % (v/v) heat-inactivated fetal bovine serum was developed. In addition to adhesive bone marrow cells, the co-existence of non-adhesive bone marrow cells was effective in expressing liver-specific functions for at least 3 weeks. On the other hand, experiments with Transwell in which cultured cells were separated by a semi-permeable membrane, suggested that soluble factors secreted by BMCs are the key components in the functional enhancement of cells. Furthermore, direct contact between hepatocytes and BMCs enhanced the formation of spheroids and the expression of liver specific functions. These results indicate that this co-culture system is promising in, for example, bioartificial liver, regenerative medicine, and liver function simulator applications.

AB - In this study, a co-culture system of hepatocytes and bone marrow cells (BMCs) was developed and characterized for the expression and maintenance of ammonia metabolism and albumin secretion activities. A culture medium supplemented with epidermal growth factor, insulin, L-proline, hydrocortisone and 20 % (v/v) heat-inactivated fetal bovine serum was developed. In addition to adhesive bone marrow cells, the co-existence of non-adhesive bone marrow cells was effective in expressing liver-specific functions for at least 3 weeks. On the other hand, experiments with Transwell in which cultured cells were separated by a semi-permeable membrane, suggested that soluble factors secreted by BMCs are the key components in the functional enhancement of cells. Furthermore, direct contact between hepatocytes and BMCs enhanced the formation of spheroids and the expression of liver specific functions. These results indicate that this co-culture system is promising in, for example, bioartificial liver, regenerative medicine, and liver function simulator applications.

KW - Albumin secretion

KW - Ammonia metabolism

KW - Bone marrow cells

KW - Co-culture

KW - Heat-inactivated fetal bovine serum

KW - Hepatocyte

KW - Non-adhesive bone marrow cells

KW - Soluble factors

KW - Spheroid

KW - Transwell

UR - http://www.scopus.com/inward/record.url?scp=1842556200&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1842556200&partnerID=8YFLogxK

M3 - Article

VL - 27

SP - 118

EP - 126

JO - International Journal of Artificial Organs

JF - International Journal of Artificial Organs

SN - 0391-3988

IS - 2

ER -