Development of an enzyme immunoassay for urinary pregnanediol-3-glucuronide in a female giant panda (Ailuropoda melanoleuca)

Natsuki Hama, Hideyasu Kanemitsu, Michiyo Tanikawa, Masami Shibaya, Kensuke Sakamoto, Yujiro Oyama, Tomas J. Acosta, Osamu Ishikawa, Wang Pengyan, Kiyoshi Okuda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In order to enable monitoring of the reproductive status of the female giant panda after observation of estrus behavior, we developed an enzyme immunoassay (EIA) system for urinary pregnanediol-3-glucuronide (PdG), a progesterone metabolite, using commercial reagents and examined the changes in the urinary concentration of PdG in a female giant panda that showed pseudopregnancy and suspicious pseudopregnancy in 6 consecutive years. The developed EIA system had good reproducibility (intra- and interassay CVs 6.1% and 16.3%, respectively), good parallelism between the standard curve and the dose response curve of serial diluted samples and positive correlation (r=0.836) with the data for PdG in the same samples measured by gas chromatography. Urinary PdG in the female panda showed two phases of increase. The first elevation was observed immediately after estrus with the levels of PdG below 100 ng/ Crmg, while the second phase was characterized by a drastic elevation above 100 ng/Crmg until the level began to decrease at the end of pseudopregnancy or suspicious pseudopregnancy. The length of the second phase had wider range than that of the first phase. In the present study, a new EIA assay system for urinary PdG in the female giant panda was developed, and we found that the length of the second phase is unstable in the pseudopregnant and suspicious pseudopregnant giant panda, in contrast with the unstable length of the first phase caused by delayed implantation in the pregnant giant panda.

Original languageEnglish
Pages (from-to)879-884
Number of pages6
JournalJournal of Veterinary Medical Science
Volume71
Issue number7
DOIs
Publication statusPublished - Aug 1 2009

Keywords

  • Enzyme immunoassay
  • Giant panda
  • PdG
  • Pseudopregnancy

ASJC Scopus subject areas

  • veterinary(all)

Fingerprint Dive into the research topics of 'Development of an enzyme immunoassay for urinary pregnanediol-3-glucuronide in a female giant panda (Ailuropoda melanoleuca)'. Together they form a unique fingerprint.

  • Cite this

    Hama, N., Kanemitsu, H., Tanikawa, M., Shibaya, M., Sakamoto, K., Oyama, Y., Acosta, T. J., Ishikawa, O., Pengyan, W., & Okuda, K. (2009). Development of an enzyme immunoassay for urinary pregnanediol-3-glucuronide in a female giant panda (Ailuropoda melanoleuca). Journal of Veterinary Medical Science, 71(7), 879-884. https://doi.org/10.1292/jvms.71.879