Development of an active targeting liposome encapsulated with high-density colloidal gold for transmission electron microscopy

Hideki Minematsu, Takayuki Otani, Toshitaka Oohashi, Masahiko Hirai, Kazunori Oie, Koichi Igarashi, Aiji Ohtsuka

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Active targeting of the liposome is an attractive strategy for drug delivery and in vivo bio-imaging. We previously reported the specific accumulation of Sialyl Lewis X (SLX) liposome to inflamed tissue in arthritic model mice or tumor-bearing mice. SLX-liposome encapsulation with fluorescent substances allows for the visualization of these liposomes by the time-dependent transvascular accumulation of fluorescent signals in the histological sections. In the present study, we developed a new SLX-liposome encapsulated with colloidal gold for transmission electron microscopic observation. We herein describe the characterization of the colloidal gold-loaded SLX-liposomes and demonstrate its specific targeting to the endothelial cells of tumor blood vessels in tumor-bearing mice.

Original languageEnglish
Pages (from-to)95-99
Number of pages5
JournalJournal of Electron Microscopy
Volume60
Issue number1
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Gold Colloid
Liposomes
Transmission Electron Microscopy
mice
tumors
Gold
gold
Transmission electron microscopy
transmission electron microscopy
Bearings (structural)
Tumors
blood vessels
delivery
drugs
Vascular Tissue Neoplasms
Endothelial cells
Blood vessels
Drug delivery
Encapsulation
Arthritis

Keywords

  • active targeting liposome
  • arthritis
  • colloidal gold
  • drug delivery
  • electron microscopy
  • tumor

ASJC Scopus subject areas

  • Instrumentation

Cite this

Development of an active targeting liposome encapsulated with high-density colloidal gold for transmission electron microscopy. / Minematsu, Hideki; Otani, Takayuki; Oohashi, Toshitaka; Hirai, Masahiko; Oie, Kazunori; Igarashi, Koichi; Ohtsuka, Aiji.

In: Journal of Electron Microscopy, Vol. 60, No. 1, 02.2011, p. 95-99.

Research output: Contribution to journalArticle

Minematsu, Hideki ; Otani, Takayuki ; Oohashi, Toshitaka ; Hirai, Masahiko ; Oie, Kazunori ; Igarashi, Koichi ; Ohtsuka, Aiji. / Development of an active targeting liposome encapsulated with high-density colloidal gold for transmission electron microscopy. In: Journal of Electron Microscopy. 2011 ; Vol. 60, No. 1. pp. 95-99.
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