Development of a,b-plane-oriented hydroxyapatite ceramics as models for living bones and their cell adhesion behavior

Zhi Zhuang, Takahiko J. Fujimi, Mariko Nakamura, Toshiisa Konishi, Hideyuki Yoshimura, Mamoru Aizawa

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

In vertebrate bones and tooth enamel surfaces, the respective a,b-planes and c-planes of hydroxyapatite (HAp) crystals are preferentially exposed. However, the reason why the HAp crystals show different orientations depending on the type of hard tissues is not yet understood. To clarify this question, appropriate ceramic models with highly preferred orientation are necessary. In the present study, dense HAp ceramic models which have the same orientation as living bones were fabricated using composite powders of c-axis-oriented single-crystal apatite fibers (AF) and wet-synthesized apatite gels (AG). The results of crystalline identification and ultrastructural observation showed that the resulting HAp ceramics maintained the c-axis orientation of the AF particles, and their high a,b-plane orientation degrees could be maintained with small additive amounts of AG; however, when the AG content was over 30 mass%, this value decreased. The influence of orientation degree on the surface characteristics was investigated by evaluating the surface zeta-potential and wettability. These results show that increasing the a,b-plane orientation degree shifted the surface charge from negative to positive, and decreased the surface wettability. Initial cell-attachment assays were performed on these resulting ceramics using MC3T3-E1 cells as models of osteoblasts. The results show that the cell-attachment efficiency decreased with increasing a,b-plane orientation degree.

Original languageEnglish
Pages (from-to)6732-6740
Number of pages9
JournalActa Biomaterialia
Volume9
Issue number5
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Apatites
Cell adhesion
Apatite
Ceramics
Durapatite
Hydroxyapatite
Cell Adhesion
Bone
Bone and Bones
Wettability
Gels
Wetting
Tooth enamel
Crystals
Fibers
Osteoblasts
Zeta potential
Dental Enamel
Surface charge
Crystal orientation

Keywords

  • Cell adhesion
  • Ceramic ultrastructure
  • Hydroxyapatite
  • Orientation
  • Surface characterization

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Development of a,b-plane-oriented hydroxyapatite ceramics as models for living bones and their cell adhesion behavior. / Zhuang, Zhi; Fujimi, Takahiko J.; Nakamura, Mariko; Konishi, Toshiisa; Yoshimura, Hideyuki; Aizawa, Mamoru.

In: Acta Biomaterialia, Vol. 9, No. 5, 2013, p. 6732-6740.

Research output: Contribution to journalArticle

Zhuang, Zhi ; Fujimi, Takahiko J. ; Nakamura, Mariko ; Konishi, Toshiisa ; Yoshimura, Hideyuki ; Aizawa, Mamoru. / Development of a,b-plane-oriented hydroxyapatite ceramics as models for living bones and their cell adhesion behavior. In: Acta Biomaterialia. 2013 ; Vol. 9, No. 5. pp. 6732-6740.
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