TY - JOUR
T1 - Development of a simple method for detection of the HLA-A*31:01 allele
AU - Uchiyama, Kazuki
AU - Kubota, Fumika
AU - Ariyoshi, Noritaka
AU - Matsumoto, Jun
AU - Ishii, Itsuko
AU - Kitada, Mitsukazu
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2013
Y1 - 2013
N2 - It is known that rare but severe cutaneous adverse drug reactions (cADRs), such as Stevens- Johnson syndrome (SJS), toxic epidermal necrolysis (TEN) and drug-induced hypersensitivity syndrome (DIHS), are induced by carbamazepine (CBZ). Recent studies have shown an association between HLAA* 31:01 and CBZ-induced severe cADRs in Japanese and Caucasian populations. In this study, we developed a simple method to detect the HLA-A*31:01 allele by nested allele-specific primer-polymerase chain reaction combined with restriction fragment length polymorphism analysis. Accuracy of the developed method was evaluated by direct sequencing analysis of PCR products amplified from DNA samples with known HLA-A genotypes and by consigning diagnosis of DNA samples with unknown HLA-A genotypes to a company providing clinical laboratory testing. The method developed in this study is simple, rapid, and of low cost compared to outsourcing tests and may be useful for in-house testing of the HLA-A*31:01 allele.
AB - It is known that rare but severe cutaneous adverse drug reactions (cADRs), such as Stevens- Johnson syndrome (SJS), toxic epidermal necrolysis (TEN) and drug-induced hypersensitivity syndrome (DIHS), are induced by carbamazepine (CBZ). Recent studies have shown an association between HLAA* 31:01 and CBZ-induced severe cADRs in Japanese and Caucasian populations. In this study, we developed a simple method to detect the HLA-A*31:01 allele by nested allele-specific primer-polymerase chain reaction combined with restriction fragment length polymorphism analysis. Accuracy of the developed method was evaluated by direct sequencing analysis of PCR products amplified from DNA samples with known HLA-A genotypes and by consigning diagnosis of DNA samples with unknown HLA-A genotypes to a company providing clinical laboratory testing. The method developed in this study is simple, rapid, and of low cost compared to outsourcing tests and may be useful for in-house testing of the HLA-A*31:01 allele.
KW - Carbamazepine
KW - HLA genotyping
KW - HLA-A31:01
KW - Idiosyncratic adverse drug reaction
KW - Stevens-Johnson syndrome
UR - http://www.scopus.com/inward/record.url?scp=84886416692&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84886416692&partnerID=8YFLogxK
U2 - 10.2133/dmpk.DMPK-12-NT-136
DO - 10.2133/dmpk.DMPK-12-NT-136
M3 - Article
C2 - 23399568
AN - SCOPUS:84886416692
VL - 28
SP - 435
EP - 438
JO - Drug Metabolism and Pharmacokinetics
JF - Drug Metabolism and Pharmacokinetics
SN - 1347-4367
IS - 5
ER -