Development of a simple method for detection of the HLA-A*31:01 allele

Kazuki Uchiyama, Fumika Kubota, Noritaka Ariyoshi, Jun Matsumoto, Itsuko Ishii, Mitsukazu Kitada

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

It is known that rare but severe cutaneous adverse drug reactions (cADRs), such as Stevens- Johnson syndrome (SJS), toxic epidermal necrolysis (TEN) and drug-induced hypersensitivity syndrome (DIHS), are induced by carbamazepine (CBZ). Recent studies have shown an association between HLAA* 31:01 and CBZ-induced severe cADRs in Japanese and Caucasian populations. In this study, we developed a simple method to detect the HLA-A*31:01 allele by nested allele-specific primer-polymerase chain reaction combined with restriction fragment length polymorphism analysis. Accuracy of the developed method was evaluated by direct sequencing analysis of PCR products amplified from DNA samples with known HLA-A genotypes and by consigning diagnosis of DNA samples with unknown HLA-A genotypes to a company providing clinical laboratory testing. The method developed in this study is simple, rapid, and of low cost compared to outsourcing tests and may be useful for in-house testing of the HLA-A*31:01 allele.

Original languageEnglish
Pages (from-to)435-438
Number of pages4
JournalDrug metabolism and pharmacokinetics
Volume28
Issue number5
DOIs
Publication statusPublished - 2013
Externally publishedYes

Keywords

  • Carbamazepine
  • HLA genotyping
  • HLA-A*31:01
  • Idiosyncratic adverse drug reaction
  • Stevens-Johnson syndrome

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

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