Development of a Sensitive Enzyme Immunoassay for Opc-7251, A Novel Antimicrobial Agent for Percutaneous Application

Norio Muto, Yoshihiro Mitoh, Itaru Yamamoto

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A sensitive enzyme immunoassay for OPC-7251, a novel pyridone carboxylic acid antimicrobial agent, was developed and applied for the determination of human plasma levels. OPC-7251 was coupled to bovine serum albumin through a formation of N-hydroxysuccinimide ester. By immunization of rabbits, highly specific antiserum was raised. Using the antiserum and B-D-galactosidase-labeled hapten, the homologous assay system allowed the detection of 2 pg of this compound. Plasma samples were precisely analyzed down to the minimum value of 200 pg/ml after heat treatment. The system was further validated by the recovery test and correlation with the HPLC analyses. Percutaneous application of 10 g of 1% OPC-7251 cream to healthy volunteers resulted in the peak plasma value of 1.6 ng/ml about 8 hours after dosing, indicating extremely low absorption efficiency through a transdermal system. (KEY WORDS: Enzyme immunoassay, ß-D-Galactosidase OPC-7251, Antimicrobial agent, Percutaneous application, Polystyrene ball).

Original languageEnglish
Pages (from-to)1-16
Number of pages16
JournalJournal of Immunoassay
Volume11
Issue number1
DOIs
Publication statusPublished - Mar 1 1990

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nadifloxacin
Anti-Infective Agents
Immunoenzyme Techniques
Galactosidases
Enzymes
4 alpha-glucanotransferase
Immune Sera
Pyridones
Plasma (human)
Immunization
Plasmas
Haptens
Polystyrenes
Bovine Serum Albumin
Carboxylic Acids
Assays
Healthy Volunteers
Esters
Hot Temperature
Heat treatment

ASJC Scopus subject areas

  • Immunology
  • Pharmacology

Cite this

Development of a Sensitive Enzyme Immunoassay for Opc-7251, A Novel Antimicrobial Agent for Percutaneous Application. / Muto, Norio; Mitoh, Yoshihiro; Yamamoto, Itaru.

In: Journal of Immunoassay, Vol. 11, No. 1, 01.03.1990, p. 1-16.

Research output: Contribution to journalArticle

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abstract = "A sensitive enzyme immunoassay for OPC-7251, a novel pyridone carboxylic acid antimicrobial agent, was developed and applied for the determination of human plasma levels. OPC-7251 was coupled to bovine serum albumin through a formation of N-hydroxysuccinimide ester. By immunization of rabbits, highly specific antiserum was raised. Using the antiserum and B-D-galactosidase-labeled hapten, the homologous assay system allowed the detection of 2 pg of this compound. Plasma samples were precisely analyzed down to the minimum value of 200 pg/ml after heat treatment. The system was further validated by the recovery test and correlation with the HPLC analyses. Percutaneous application of 10 g of 1{\%} OPC-7251 cream to healthy volunteers resulted in the peak plasma value of 1.6 ng/ml about 8 hours after dosing, indicating extremely low absorption efficiency through a transdermal system. (KEY WORDS: Enzyme immunoassay, {\ss}-D-Galactosidase OPC-7251, Antimicrobial agent, Percutaneous application, Polystyrene ball).",
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