Development of a real-time loop-mediated isothermal amplification assay for the sensitive and rapid detection of Listeria monocytogenes

L. Ye, Y. Li, J. Zhao, Z. Zhang, H. Meng, H. Yan, Shin-ichi Miyoshi, L. Shi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A real-time loop-mediated isothermal amplification (RealAmp) assay for the detection of Listeria was developed. The RealAmp assay, using primers specific for the hemolysin-encoding hlyA gene, was verified using Listeria monocytogenes strains (n = 58) from different regions of the world. Both the sensitivity and specificity of the RealAmp assay were high. The RealAmp assay could detect 103 CFU ml-1 within 30 min. A comparative evaluation of the RealAmp assay, the API Listeria assay, and the real-time PCR assay revealed that the RealAmp assay is simpler, faster, and has a higher specificity than the other two assays.

Original languageEnglish
Pages (from-to)85-90
Number of pages6
JournalLetters in Applied Microbiology
Volume61
Issue number1
DOIs
Publication statusPublished - Jul 1 2015

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Keywords

  • Listeria monocytogenes
  • API Listeria
  • ESEQuant tube scanner
  • Real-time PCR
  • RealAmp

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Medicine(all)

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