Development of a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells

Heng Wei, Chen Wang, Rui Guo, Ken Takahashi, Keiji Naruse

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Ischemic heart disease remains the largest cause of death worldwide. Accordingly, many researchers have sought curative options, often using laboratory animal models such as rodents. However, the physiology of the human heart differs significantly from that of the rodent heart. In this study, we developed a model of ischemic heart disease using cardiomyocytes differentiated from human induced pluripotent stem cells (hiPS-CMs). After optimizing the conditions of ischemia, including the concentration of oxygen and duration of application, we evaluated the consequent damage to hiPS-CMs. Notably, exposure to 2% oxygen, 0 mg/ml glucose, and 0% fetal bovine serum increased the percentage of nuclei stained with propidium iodide, an indicator of membrane damage, and decreased cellular viability. These conditions also decreased the contractility of hiPS-CMs. Furthermore, ischemic conditioning increased the mRNA expression of IL-8, consistent with observed conditions in the in vivo heart. Taken together, these findings suggest that our hiPS-CM-based model can provide a useful platform for human ischemic heart disease research.

Original languageEnglish
Pages (from-to)600-605
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume520
Issue number3
DOIs
Publication statusPublished - Dec 10 2019

Keywords

  • Cardiomyocytes
  • Human induced pluripotent stem cells
  • Ischemic heart disease
  • Myocardial infarction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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