Development of a method for the identification of S-IgA-coated bacterial composition in mouse and human feces

Takeshi Tsuruta, Ryo Inoue, Toshihiko Iwanaga, Hiroshi Hara, Takaji Yajima

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Some commensal intestinal bacteria in humans and mice are coated with secretory immunoglobulin A (S-IgA). It has been suggested that the S-IgA coating of commensal bacteria does not occur at random and thus identification of S-IgA-coated bacterial genera/species should help in elucidating the interaction between S-IgA and commensal intestinal bacteria, but no method of identifying the genera/species of S-IgAcoated bacteria has been established. To identify S-IgAcoated bacterial composition, we developed a method combining immunohistochemical detection of S-IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Human and mouse fecal S-IgA coated bacterial composition was evaluated by this newly developed method with 10 frequently-used FISH probes. Fecal S-IgA-coated bacterial composition was successfully analyzed by this method, and this suggests that Enterobacteriaceae is preferably coated with S-IgA, whereas Bacteroides/Prevotella and Lactobacillus/Enterococcus groups appear to be poorly coated with S-IgA.

Original languageEnglish
Pages (from-to)968-973
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number5
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Secretory Immunoglobulin A
Feces
Chemical analysis
Bacteria
Fluorescence In Situ Hybridization
Fluorescence
Prevotella
Bacteroides
Enterococcus
Lactobacillus
Enterobacteriaceae
Immunoglobulin A
Coatings

Keywords

  • Commensal intestinal bacteria
  • Diamino-benzidine immunohistochemical detection
  • Fluorescence in situ hybridization
  • Secretary immunoglobulin A

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Development of a method for the identification of S-IgA-coated bacterial composition in mouse and human feces. / Tsuruta, Takeshi; Inoue, Ryo; Iwanaga, Toshihiko; Hara, Hiroshi; Yajima, Takaji.

In: Bioscience, Biotechnology and Biochemistry, Vol. 74, No. 5, 2010, p. 968-973.

Research output: Contribution to journalArticle

Tsuruta, Takeshi ; Inoue, Ryo ; Iwanaga, Toshihiko ; Hara, Hiroshi ; Yajima, Takaji. / Development of a method for the identification of S-IgA-coated bacterial composition in mouse and human feces. In: Bioscience, Biotechnology and Biochemistry. 2010 ; Vol. 74, No. 5. pp. 968-973.
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