Development of a method for the identification of S-IgA-coated bacterial composition in mouse and human feces

Takeshi Tsuruta, Ryo Inoue, Toshihiko Iwanaga, Hiroshi Hara, Takaji Yajima

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Some commensal intestinal bacteria in humans and mice are coated with secretory immunoglobulin A (S-IgA). It has been suggested that the S-IgA coating of commensal bacteria does not occur at random and thus identification of S-IgA-coated bacterial genera/species should help in elucidating the interaction between S-IgA and commensal intestinal bacteria, but no method of identifying the genera/species of S-IgAcoated bacteria has been established. To identify S-IgAcoated bacterial composition, we developed a method combining immunohistochemical detection of S-IgA and subsequent 16S rRNA targeted fluorescence in situ hybridization (FISH) analysis. Human and mouse fecal S-IgA coated bacterial composition was evaluated by this newly developed method with 10 frequently-used FISH probes. Fecal S-IgA-coated bacterial composition was successfully analyzed by this method, and this suggests that Enterobacteriaceae is preferably coated with S-IgA, whereas Bacteroides/Prevotella and Lactobacillus/Enterococcus groups appear to be poorly coated with S-IgA.

Original languageEnglish
Pages (from-to)968-973
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume74
Issue number5
DOIs
Publication statusPublished - Aug 13 2010

    Fingerprint

Keywords

  • Commensal intestinal bacteria
  • Diamino-benzidine immunohistochemical detection
  • Fluorescence in situ hybridization
  • Secretary immunoglobulin A

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

Cite this